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  • Organism:saccharomyces cerevisiae (facet)

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pET28b-sad1-D422A
 
Resource Report
Resource Website 		RRID:Addgene_90307 SAD1 Saccharomyces cerevisiae Kanamycin PMID:24681967 Full-length Sad1, amino acids 1–448, was cloned from S. cerevisiae S288C into the pET-28b plasmid at the Bam HI and XhoI sites, which fuses a 6-His tag to the N terminus. Backbone Marker:EMD Biosciences; Backbone Size:5368; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin D422A 2026-08-15 01:22:06 0
pET28b-sad1-H405A
 
Resource Report
Resource Website 		RRID:Addgene_90306 SAD1 Saccharomyces cerevisiae Kanamycin PMID:24681967 Full-length Sad1, amino acids 1–448, was cloned from S. cerevisiae S288C into the pET-28b plasmid at the Bam HI and XhoI sites, which fuses a 6-His tag to the N terminus. Backbone Marker:EMD Biosciences; Backbone Size:5368; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin H405A 2026-08-15 01:22:06 0
pET28b-sad1-C63A
 
Resource Report
Resource Website 		RRID:Addgene_90302 SAD1 Saccharomyces cerevisiae Kanamycin PMID:24681967 Full-length Sad1, amino acids 1–448, was cloned from S. cerevisiae S288C into the pET-28b plasmid at the Bam HI and XhoI sites, which fuses a 6-His tag to the N terminus. Backbone Marker:EMD Biosciences; Backbone Size:5368; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin C63A 2026-08-15 01:22:06 0
pSL14 - Prp8 2142–2398
 
Resource Report
Resource Website 		RRID:Addgene_90264 PRP8 Saccharomyces cerevisiae Ampicillin PMID:27072132 Ledoux and Guthrie, JBC, 2016 Backbone Marker:ThermoFisher Scientific; Vector Backbone:pDEST15; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin only Jab1 domain (aa 2143-2398) 2026-08-15 01:22:06 0
pSL13 - Brr2 del422 R1107L
 
Resource Report
Resource Website 		RRID:Addgene_90263 BRR2 Saccharomyces cerevisiae Ampicillin PMID:27072132 Ledoux and Guthrie, JBC, 2017 Backbone Size:4957; Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin deleted first 422 amino acids and amino acid R1107L 2026-08-15 01:22:06 0
pSL10 - Brr2 del422 WT
 
Resource Report
Resource Website 		RRID:Addgene_90260 BRR2 Saccharomyces cerevisiae Ampicillin PMID:27072132 Ledoux and Guthrie, JBC, 2017 Backbone Size:4957; Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin deleted first 422 amino acids 2026-08-15 01:22:06 0
pSL11 - Brr2 del422 N1104L
 
Resource Report
Resource Website 		RRID:Addgene_90261 BRR2 Saccharomyces cerevisiae Ampicillin PMID:27072132 Ledoux and Guthrie, JBC, 2017 Backbone Size:4957; Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin deleted first 422 amino acids and amino acid N1104L 2026-08-15 01:22:06 0
Sub2-4 in pRS314
 
Resource Report
Resource Website 		RRID:Addgene_90455 SUB2 Saccharomyces cerevisiae Ampicillin PMID:11156604 Addgene NGS results found E16G, H60Y, A114T, K202E, E222G, and E267G in the SUB2 translation compared to NP_010199.1 Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin sub2-4 mutation 2026-08-15 01:22:07 0
pWS172
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_90519 Cas9 Saccharomyces cerevisiae Kanamycin For more information see https://benchling.com/pub/ellis-crispr-tools Backbone Size:3882; Vector Backbone:2 micron; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Kanamycin 2026-08-15 01:22:07 1
pWS082
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_90516 Yeast sgRNA expression vector Saccharomyces cerevisiae Ampicillin Refer to https://benchling.com/pub/ellis-crispr-tools for guidelines on how to use Backbone Size:1753; Vector Backbone:N/A; Vector Types:Yeast Expression, CRISPR, Yeast gap repair; Bacterial Resistance:Ampicillin 2026-08-15 01:22:07 6
pRS313 - prp8 V1870N
 
Resource Report
Resource Website 		RRID:Addgene_90976 PRP8 Saccharomyces cerevisiae Ampicillin PMID:28416677 Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin V1870N 2026-08-15 01:22:13 0
pRS313 - prp8 N1869D
 
Resource Report
Resource Website 		RRID:Addgene_90975 PRP8 Saccharomyces cerevisiae Ampicillin PMID:28416677 Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin N1869D 2026-08-15 01:22:13 0
pRS313 - prp8 I1875T
 
Resource Report
Resource Website 		RRID:Addgene_90977 PRP8 Saccharomyces cerevisiae Ampicillin PMID:28416677 Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin I1875T 2026-08-15 01:22:13 0
pRS313 - prp8 K1864E
 
Resource Report
Resource Website 		RRID:Addgene_90974 PRP8 Saccharomyces cerevisiae Ampicillin PMID:28416677 Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin K1864E 2026-08-15 01:22:13 0
pRS313 - prp8 T1872E
 
Resource Report
Resource Website 		RRID:Addgene_90970 PRP8 Saccharomyces cerevisiae Ampicillin PMID:28416677 Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin T1872E 2026-08-15 01:22:13 0
pRS313 - prp8 A1871E
 
Resource Report
Resource Website 		RRID:Addgene_90969 PRP8 Saccharomyces cerevisiae Ampicillin PMID:28416677 Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin A1871E 2026-08-15 01:22:13 0
pRS313 - prp8 T1865K
 
Resource Report
Resource Website 		RRID:Addgene_90968 PRP8 Saccharomyces cerevisiae Ampicillin PMID:28416677 Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin T1865K 2026-08-15 01:22:13 0
pRS313 - prp8 V1862D
 
Resource Report
Resource Website 		RRID:Addgene_90967 PRP8 Saccharomyces cerevisiae Ampicillin PMID:28416677 Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin V1862D 2026-08-15 01:22:13 0
pWS173
 
Resource Report
Resource Website 		RRID:Addgene_90960 Cas9 Saccharomyces cerevisiae Kanamycin For more information see https://benchling.com/pub/ellis-crispr-tools Backbone Size:4163; Vector Backbone:2 micron; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Kanamycin 2026-08-15 01:22:13 0
pWS176
 
Resource Report
Resource Website 		RRID:Addgene_90963 Cas9 Saccharomyces cerevisiae Kanamycin For more information see https://benchling.com/pub/ellis-crispr-tools Backbone Size:3728; Vector Backbone:2 micron; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Kanamycin 2026-08-15 01:22:13 0

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