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Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_128060 Copy
Vector Backbone Description: Vector Backbone:plenti guide puro addgene 52963; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_128063 Copy
Vector Backbone Description: Vector Backbone:pLenti6-V5-Topo; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: The V5 tag in this plasmid backbone is not fused to mCherry.
Proper citation: RRID:Addgene_128062 Copy
Vector Backbone Description: Vector Backbone:pLVX-Tet3G; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_128061 Copy
Vector Backbone Description: Vector Backbone:pCDH-EF1-IRES cop GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: Addgene NGS analysis identified 3 lentiviral ITR sequences. Depositing lab has indicated that this plasmid is functional.
Proper citation: RRID:Addgene_128059 Copy
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pAWP78; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26902345
Comments: pAWP78 was gifted from Mary Lidstrom's laboratory at the University of Washington
Proper citation: RRID:Addgene_128162 Copy
Species: Other
Genetic Insert: Sar1
Vector Backbone Description: Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33852913
Comments: Sar1-T39N is a dominant-negative mutant
Proper citation: RRID:Addgene_128155 Copy
Species: Rattus norvegicus
Genetic Insert: Dynamin 2
Vector Backbone Description: Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19948954
Comments: Please note: Plasmid contains D406G, V664A, H692R, and Q758R mutations in Dynamin2 compared to the NCBI reference sequence XP_006242670.1. These mutations are not known to affect plasmid function.
Proper citation: RRID:Addgene_128153 Copy
Vector Backbone Description: Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_128144 Copy
Species: Rattus norvegicus
Genetic Insert: TGN38
Vector Backbone Description: Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_128148 Copy
Vector Backbone Description: Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_128147 Copy
Species: Synthetic
Genetic Insert: rtTA-Advanced::P2A::EGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5070; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression, Synthetic Biology, Flp-In expression vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31235692
Comments: The plasmid encodes a mammalian synthetic gene circuit that contains the regulator reverse tetracycline Trans Activator (rtTA) and the reporter EGFP, which is controlled by a tight TRE promoter that rtTA binds to activate its own expression in a positive feedback loop. The backbone consists of the Flp-In expression vector (pcDNA5/FRT). The synthetic gene circuit is also called mPF-GFP.
Proper citation: RRID:Addgene_128254 Copy
Species: Synthetic
Genetic Insert: Beta Globin Intron::hTetR::P2A::EGFP
Vector Backbone Description: Backbone Marker:Custom; Backbone Size:7505; Vector Backbone:pDN-D2irTNG5kwh; Vector Types:Mammalian Expression, Synthetic Biology, Flp-In expression vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31235692
Comments: The plasmid encodes a mammalian synthetic gene circuit with the humanized Tetracycline Repressor (hTetR) regulator separated from EGFP by a P2A motif, which is controlled by a CMV-derived promoter containing 2 tetO2 sites. With hTetR binding to the promoter, negative feedback lowers gene expression noise while linearizing the mean dose response. The circuit is also called mNF-GFP and is within a Flp-In expression vector.
Proper citation: RRID:Addgene_128253 Copy
Species: Homo sapiens
Genetic Insert: TEX264 LIR4A
Vector Backbone Description: Backbone Marker:Dr.Shoji Yamaoka of Tokyo Medical and Dental University; Backbone Size:6404; Vector Backbone:pMRX-INU-FLAG; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31006538
Proper citation: RRID:Addgene_128259 Copy
Species: Homo sapiens
Genetic Insert: TEX264
Vector Backbone Description: Backbone Marker:Dr.Shoji Yamaoka of Tokyo Medical and Dental University; Backbone Size:6404; Vector Backbone:pMRX-INU-FLAG; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31006538
Proper citation: RRID:Addgene_128258 Copy
Species: Other
Genetic Insert: ssRFPGFPKDEL
Vector Backbone Description: Backbone Size:7710; Vector Backbone:pCW57-CMV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31006538
Proper citation: RRID:Addgene_128257 Copy
Species: Synthetic
Genetic Insert: ZmUBIp::ScCas9 (wheat codon optimized):NOSt
Vector Backbone Description: Vector Backbone:pICH47742; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128192 Copy
Species: Rhodosporidium toruloides
Genetic Insert: gRNA cloning cassette
Vector Backbone Description: Backbone Size:6303; Vector Backbone:pZPK; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31038202
Proper citation: RRID:Addgene_128178 Copy
Species: Rhodosporidium toruloides
Genetic Insert: SpCas9-NLS3
Vector Backbone Description: Backbone Marker:Zhao lab; Backbone Size:13658; Vector Backbone:pCAMBRIA; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31038202
Proper citation: RRID:Addgene_128177 Copy
Species: Homo sapiens
Genetic Insert: shcGAS Mmu
Vector Backbone Description: Backbone Size:13320; Vector Backbone:pTRIPZ RFP+; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28598415
Proper citation: RRID:Addgene_128175 Copy
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