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Species: Other
Genetic Insert: Venus
Vector Backbone Description: Backbone Marker:Muller Lab, Addgene plasmid 83777; Vector Backbone:pDH6; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12073355
Comments: For more information, see http://yeastrc.org/yeastrc/pages/plasmids_protocols.html
Plasmid pBS7 is built from pDH6 except YFP has been mutated to the Venus variant. Those mutations are described in "A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications" by Nagai, Ibata, Park, Kubota, Mikoshiba and Miyawaki in Nat Biotechnol 2002 Jan;20(1):87-90. In addition we have included Q69M, a mutation briefly mentioned in the Nagai et al. paper as potentially beneficial, although not necessary for the enhanced maturation.
Proper citation: RRID:Addgene_83774 Copy
Species: Other
Genetic Insert: YFP
Vector Backbone Description: Backbone Marker:Wach, et al. 1997, PMID 9290211; Backbone Size:4726; Vector Backbone:pFA6a-GFP(S65T)-HIS; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12073355
Comments: For more information, see http://yeastrc.org/yeastrc/pages/plasmids_protocols.html
Proper citation: RRID:Addgene_83775 Copy
Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pFA6-GFPkan; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12073355
Comments: For more information, see http://yeastrc.org/yeastrc/pages/plasmids_protocols.html
Proper citation: RRID:Addgene_83796 Copy
Species: Other
Genetic Insert: YFP
Vector Backbone Description: Backbone Marker:Prein et al. 2000, PMID 11086160; Vector Backbone:pyGFP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12073355
Comments: For more information, see http://yeastrc.org/yeastrc/pages/plasmids_protocols.html
Proper citation: RRID:Addgene_83798 Copy
Species: Other
Genetic Insert: CFP
Vector Backbone Description: Backbone Marker:Muller Lab; Backbone Size:4875; Vector Backbone:pDH3 (Addgene plasmid 83776 ); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12073355
Comments: For more information, see http://yeastrc.org/yeastrc/pages/plasmids_protocols.html
Proper citation: RRID:Addgene_83793 Copy
Species: Other
Genetic Insert: TetO-GFP
Vector Backbone Description: Vector Backbone:Lentiviral vector (SIV derived); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21750535
Comments: Can be used to produce inducible SIV-derived vector. Packaging constructs can be derived from HIV-1 without loss of titer and tranduction efficiencies in cell line. Cells tranduced by lentiviral particles will overexpress GFP upon activation of the tetO promoter. This activation can be ensured by a constitutive expression of the tTA in recipient cells or by addition of tTA-gesicles. Expression of GFP is low without activation, depending on the recipient-cell type.
GFP can be replaced using Age1 and BamH1.
Proper citation: RRID:Addgene_83958 Copy
Species: Other
Genetic Insert: Cre
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Cre/Lox, Zebrafish transgenesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22908272
Comments: Designed in a pBluescript series vector containing I-SceI meganuclease sites. The I-SceI meganuclease method is a common method for establishing transgenic zebrafish lines.
Proper citation: RRID:Addgene_84033 Copy
Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:6604; Vector Backbone:pCM29; Vector Types:shuttle vector E.coli-S.aureus, expression of sGFP from the SarA-P1 promoter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28266573
Proper citation: RRID:Addgene_84455 Copy
Species: Other
Genetic Insert: YFP venus
Vector Backbone Description: Backbone Size:6604; Vector Backbone:pCM29; Vector Types:shuttle vector E.coli-S.aureus, expression of sGFP from the SarA-P1 promoter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28266573
Proper citation: RRID:Addgene_84453 Copy
Species: Other
Genetic Insert: HyPer2 targeted to nucleus
Vector Backbone Description: Backbone Marker:Grefen et al. The Plant Journal (2010) 64, 355–365; Backbone Size:9210; Vector Backbone:pUB-DEST; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28663550
Proper citation: RRID:Addgene_84730 Copy
Species: Other
Genetic Insert: pHRed
Vector Backbone Description: Backbone Marker:Grefen et al. The Plant Journal (2010) 64, 355–365; Backbone Size:9210; Vector Backbone:pUB-DEST; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28663550
Proper citation: RRID:Addgene_84734 Copy
Species: Other
Genetic Insert: HyPer2 targeted to nucleus
Vector Backbone Description: Backbone Marker:pGWB2; Backbone Size:16636; Vector Backbone:pGemini (Dr. Andrew Simkin); Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28663550
Proper citation: RRID:Addgene_84737 Copy
Species: Other
Genetic Insert: major satellite-specific sgRNA
Vector Backbone Description: Backbone Size:5314; Vector Backbone:pEX-A; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27676121
Proper citation: RRID:Addgene_84780 Copy
Species: Other
Genetic Insert: LFn
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27045008
Proper citation: RRID:Addgene_84871 Copy
Species: Other
Genetic Insert: LFn
Vector Backbone Description: Backbone Marker:ThermoFisher; Vector Backbone:pFastBacDual; Vector Types:Insect Expression, Baculovirus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27045008
Proper citation: RRID:Addgene_84867 Copy
Species: Other
Genetic Insert: LFn
Vector Backbone Description: Backbone Marker:ThermoFisher; Vector Backbone:pFastBacDual; Vector Types:Insect Expression, Baculovirus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27045008
Proper citation: RRID:Addgene_84866 Copy
Species: Other
Genetic Insert: LFn
Vector Backbone Description: Backbone Marker:ThermoFisher; Vector Backbone:pFastBacDual; Vector Types:Insect Expression, Baculovirus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27045008
Proper citation: RRID:Addgene_84868 Copy
Species: Other
Genetic Insert: PkaiB-luciferase
Vector Backbone Description: Vector Backbone:NSI3 vector with Cm resistance expressing PkaiB-luciferase from firefly constructed by seamless cloning; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26113641
Proper citation: RRID:Addgene_85126 Copy
Species: Other
Genetic Insert: fragment of the human herpesvirus 6B strain Z29 genome
Vector Backbone Description: Backbone Marker:Stratagene, similar to pBluescript SK+ ; Vector Backbone:pKSm13-; Vector Types:cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8634027
Comments: restriction enzyme fragment = KpnI H; Range (bases) = 142,281-146,675; Associated Genes = U94, B8 (partial)
Proper citation: RRID:Addgene_85252 Copy
Species: Other
Genetic Insert: fragment of the human herpesvirus 6B strain Z29 genome
Vector Backbone Description: Backbone Marker:Stratagene, similar to pBluescript SK+ ; Vector Backbone:pKSm13-; Vector Types:cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8634027
Comments: restriction enzyme fragment = SalI R; Range (bases) = 15,692-17,012; Associated Genes = U7EX1
Proper citation: RRID:Addgene_85256 Copy
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