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Species: Other
Genetic Insert: Knockout cassette G418
Vector Backbone Description: Backbone Size:2071; Vector Backbone:pDM304; Vector Types:Cre/Lox, Dictyostelium discoideum knockout vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29847546
Comments: Due to difficulties in assembling repetitive elements within the plasmid, Addgene NGS may have some ambiguous bases and show minor differences compared to the depositor sequence. Please use the assembled depositor sequence if necessary.
Proper citation: RRID:Addgene_108982 Copy
Species: Other
Genetic Insert: Knockout cassette Nourseothricin
Vector Backbone Description: Backbone Size:2071; Vector Backbone:pDM304; Vector Types:Cre/Lox, Dictyostelium discoideum knockout vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29847546
Comments: Due to difficulties in assembling repetitive elements within the plasmid, Addgene NGS may have some ambiguous bases and show minor differences compared to the depositor sequence. Please use the assembled depositor sequence if necessary.
Proper citation: RRID:Addgene_108980 Copy
Species: Other
Genetic Insert: Cre recombinase
Vector Backbone Description: Backbone Size:6901; Vector Backbone:pDM1203; Vector Types:Cre/Lox, Cre expression vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29847546
Comments: Due to difficulties in assembling repetitive elements within the plasmid, Addgene NGS may have some ambiguous bases and show minor differences compared to the depositor sequence. Please use the assembled depositor sequence if necessary.
Proper citation: RRID:Addgene_108995 Copy
Species: Other
Genetic Insert: PTSG_07215
Vector Backbone Description: Vector Backbone:pDSB_0093; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30281390
Comments: pDSB_0101 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint.
Proper citation: RRID:Addgene_109100 Copy
Species: Other
Genetic Insert: pActAvrII_nanoluc
Vector Backbone Description: Vector Backbone:pDSB_0028; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30281390
Comments: pDSB_0029 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint.
Proper citation: RRID:Addgene_109077 Copy
Species: Other
Genetic Insert: PTSG_06009
Vector Backbone Description: Vector Backbone:pDSB_0093; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30281390
Comments: pDSB_0099 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint.
Proper citation: RRID:Addgene_109102 Copy
Species: Other
Genetic Insert: PTSG_07215 deltaCC
Vector Backbone Description: Vector Backbone:pDSB_0093; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30281390
Comments: pDSB_0109 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint.
Proper citation: RRID:Addgene_109107 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:from PMID 9218713; Vector Backbone:pSUM36; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22820329
Comments: GFP, originally modified for expression in prokaryotes and including an optimized translational initiation region from the plasmid pGreenTIR, was cloned from pGreenTIR into the HindIII site of the pSUM36 vector to generate pSum-LacZ-gfp.
Proper citation: RRID:Addgene_109285 Copy
Species: Other
Genetic Insert: GroEL-GFP
Vector Backbone Description: Backbone Marker:from PMID 9218713; Vector Backbone:pSUM36; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22820329
Comments: The groEL promoter was PCR-amplified from M. tuberculosis genomic DNA, cloned into the pCR2.1 TOPO-TA cloning vector and subsequently isolated as a BamHI/XbaI piece that was subcloned into pSUM36 cut with BamHI and NheI to remove the lacZ promoter. The GFP gene was subcloned into the HindIII site of the pSUM-groEL vector.
Proper citation: RRID:Addgene_109286 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:from PMID 9218713; Vector Backbone:pSUM36; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22820329
Comments: MCS2: HindIII and BamHI cloning sites
Proper citation: RRID:Addgene_109290 Copy
Species: Other
Genetic Insert: zCas9
Vector Backbone Description: Vector Backbone:pYPQ167; Vector Types:Plant Expression, CRISPR, Gateway compatible zCas9 entry clone; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29972722
Comments: pEGG-3_FLAG-NLS-zCas9-NLS cassette was originally from pHEE401E (Addgene plasmid#71287)
Proper citation: RRID:Addgene_109327 Copy
Species: Other
Genetic Insert: zCas9
Vector Backbone Description: Vector Backbone:pYPQ165; Vector Types:Plant Expression, CRISPR, Gateway compatible zCas9 entry clone; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29972722
Comments: 3_FLAG-NLS-zCas9-NLS cassette was originally from pHEE401E (Addgene plasmid#71287)
Proper citation: RRID:Addgene_109328 Copy
Species: Other
Genetic Insert: pRHAM-LacZ
Vector Backbone Description: Vector Backbone:pD864 (pUC origin of replication and AmpR); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29578536
Proper citation: RRID:Addgene_109380 Copy
Species: Other
Genetic Insert: pARABAD-VioB-mCherry
Vector Backbone Description: Vector Backbone:pSB1C3 (pMB1 origin of replication and CmR); Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29578536
Proper citation: RRID:Addgene_109381 Copy
Species: Other
Genetic Insert: pARABAD-VioB-mCherry
Vector Backbone Description: Vector Backbone:pLys (p15A origin of replication and CmR); Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29578536
Proper citation: RRID:Addgene_109382 Copy
Species: Other
Genetic Insert: MCS2-Myc
Vector Backbone Description: Backbone Marker:from PMID 9218713; Backbone Size:5200; Vector Backbone:pSUM36; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22820329
Proper citation: RRID:Addgene_109379 Copy
Species: Other
Genetic Insert: pibpAB-GFP
Vector Backbone Description: Vector Backbone:YTK095 (ColE1 origin of replication and AmpR); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29578536
Proper citation: RRID:Addgene_109390 Copy
Species: Other
Genetic Insert: pgroSL-GFP
Vector Backbone Description: Vector Backbone:YTK095 (ColE1 origin of replication and AmpR); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29578536
Proper citation: RRID:Addgene_109389 Copy
Species: Other
Genetic Insert: mycophage integrase
Vector Backbone Description: Vector Backbone:pMA; Vector Types:Required for transformation of attB integrating vectors; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29934571
Proper citation: RRID:Addgene_110096 Copy
Species: Other
Genetic Insert: scaffold Sybody of the concave library
Vector Backbone Description: Vector Backbone:pBXPHM3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29792401
Proper citation: RRID:Addgene_110102 Copy
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