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Species: Homo sapiens
Genetic Insert: autophagy related genes 14
Vector Backbone Description: Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:6878; Vector Backbone:pMXs-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18843052
Proper citation: RRID:Addgene_38265 Copy
Species: MW polyomavirus, strain MA095
Genetic Insert: 203-1408 of MWPyV
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:pCR4.0; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22740408
Comments: Plasmid contains nucleotides 203-1408 of GenBank ID JQ898291
Proper citation: RRID:Addgene_38782 Copy
Species: MW polyomavirus, strain MA095
Genetic Insert: 1334-2611 of MWPyV
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:pCR4.0; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22740408
Comments: Plasmid contains nucleotides 1334-2611 of GenBank ID JQ898291. The single nucleotide mismatch occurs at bp# 2606 when compared to this reference sequence.
Proper citation: RRID:Addgene_38783 Copy
Species: Homo sapiens
Genetic Insert: UV radiation resistance-associated gene protein
Vector Backbone Description: Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:6878; Vector Backbone:pMXs-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18843052
Proper citation: RRID:Addgene_38267 Copy
Species: MW polyomavirus, strain MA095
Genetic Insert: 2533-3829 of MWPyV
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:pCR4.0; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22740408
Comments: Plasmid contains nucleotides 2533-3829 of GenBank ID JQ898291.
Proper citation: RRID:Addgene_38784 Copy
Species: MW polyomavirus, strain WD976
Genetic Insert: 1346-2623 of MWPyV
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:pCR4.0; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22740408
Comments: Plasmid contains nucleotides 1346-2623 of GenBank ID JQ898292. The three nucleotide mismatches are at bp# 1858, 2617 & 2618 when compared to this reference sequence.
Proper citation: RRID:Addgene_38785 Copy
Species: Rattus norvegicus
Genetic Insert: β-arrestin2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18534983
Proper citation: RRID:Addgene_38261 Copy
Species: Homo sapiens
Genetic Insert: autophagy related genes 14
Vector Backbone Description: Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:5800; Vector Backbone:pMXs-IP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18843052
Proper citation: RRID:Addgene_38264 Copy
Species: Synthetic
Genetic Insert: E2-Crimson
Vector Backbone Description: Backbone Marker:Glick Lab; Backbone Size:4313; Vector Backbone:YIPlac204TKC-DsRed-HDEL (Addgene Plasmid #21770); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19658435
Comments: Vector for targeting E2-Crimson to the ER in yeast cells.
Linearize with EcoRV to integrate at TRP
Substitutions in E2-Crimson relative to DsRed-Express2 are: E32V, Q66F, V71A, V73I, K83L, L85Q, F118L, L150N, I161N, K163M, V175C, Y193H, S197Y
Proper citation: RRID:Addgene_38771 Copy
Genetic Insert: eGFP
Vector Backbone Description: Backbone Marker:Matthew Bogyo laboratory; Backbone Size:6126; Vector Backbone:Addgene Plasmid #38251 (pET22b-CPDSalI); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19956581
Comments: Encodes untagged eGFP fused to Vibrio cholerae MARTX toxin cysteine protease domain (CPD) to be used as a positive control for protein purification
Proper citation: RRID:Addgene_38257 Copy
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:6153; Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19956581
Comments: For protein purification, target protein of interest should be cloned into NdeI and SalI sites to create HA-tagged fusion with Vibrio cholerae MARTX toxin cysteine protease domain (CPD). HA epitope tag will remain on target protein after cleavage.
Proper citation: RRID:Addgene_38253 Copy
Species: Synthetic
Genetic Insert: E2-Crimson
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5603; Vector Backbone:pEF/myc/ER; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19658435
Comments: Vector for targeting E2-Crimson to the ER in mammalian cells.
Substitutions in E2-Crimson relative to DsRed-Express2 are: E32V, Q66F, V71A, V73I, K83L, L85Q, F118L, L150N, I161N, K163M, V175C, Y193H, S197Y
IMPORTANT NOTE: There is an 82-bp intron after the ER signal sequence. The signal sequence will be in frame with the final product.
Proper citation: RRID:Addgene_38770 Copy
Species: Homo sapiens
Genetic Insert: β₂AR
Vector Backbone Description: Vector Backbone:pcDNA3.1-mCFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16687412
Comments: The Lefkowitz Lab has confirmed this plasmid contains the human gene, and not the rat version described in the publication.
Proper citation: RRID:Addgene_38260 Copy
Species: Homo sapiens
Genetic Insert: Rif
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15668168
Comments: human Rif GTPase (RHOF) with N-terminal myc-tag in pcDNA3. Constitutively active point mutation of Q77L
Proper citation: RRID:Addgene_38768 Copy
Species: Drosophila melanogaster
Genetic Insert: zw4
Vector Backbone Description: Backbone Size:8904; Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9012507
Comments: Perrimon Lab plasmid #77
Proper citation: RRID:Addgene_38764 Copy
Species: Mus musculus
Genetic Insert: Tiar
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pLJM60; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22552098
Proper citation: RRID:Addgene_38244 Copy
Species: Drosophila melanogaster
Genetic Insert: dishevelled
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBlueScript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8288125
Comments: Perrimon Lab plasmid #17
Proper citation: RRID:Addgene_38342 Copy
Species: Drosophila melanogaster
Genetic Insert: spitz
Vector Backbone Description: Backbone Size:7855; Vector Backbone:pCaSpeR-4; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1644292
Comments: Perrimon Lab plasmid #10
Proper citation: RRID:Addgene_38340 Copy
Species: Drosophila melanogaster
Genetic Insert: djun
Vector Backbone Description: Backbone Size:8900; Vector Backbone:pCaSpeR-hs; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1696724
Comments: Perrimon Lab plasmid #7
Proper citation: RRID:Addgene_38337 Copy
Species: Mus musculus
Genetic Insert: Septin 7
Vector Backbone Description: Backbone Marker:Ambion; Backbone Size:3130; Vector Backbone:pSilencer 2.0-U6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19043408
Proper citation: RRID:Addgene_38299 Copy
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