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Species: Homo sapiens
Genetic Insert: dCas9-VP64-2A-BlastR
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene #75112); Vector Backbone:lentiSAMv2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31253982
Comments: Please visit https://www.biorxiv.org/content/early/2018/08/27/400291 for bioRxiv preprint.
Proper citation: RRID:Addgene_118207 Copy
Species: Homo sapiens
Genetic Insert: OTUB1
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30282802
Proper citation: RRID:Addgene_118209 Copy
Species: Homo sapiens
Genetic Insert: KRAB-dCas9-2A-BlastR
Vector Backbone Description: Backbone Marker:Jorge Ferrer; Vector Backbone:Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31253982
Comments: Please visit https://www.biorxiv.org/content/early/2018/08/27/400291 for bioRxiv preprint.
Note: Plasmid contains a N1396S mutation in dCas9. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_118173 Copy
Species: Homo sapiens
Genetic Insert: KRAB-dCas9-2A-BlastR
Vector Backbone Description: Backbone Marker:Jorge Ferrer; Vector Backbone:Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31253982
Comments: Please visit https://www.biorxiv.org/content/early/2018/08/27/400291 for bioRxiv preprint.
Note: Plasmid contains a N1396S mutation in dCas9. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_118174 Copy
Species: Homo sapiens
Genetic Insert: KRAB-dCas9-2A-BlastR
Vector Backbone Description: Backbone Marker:Jorge Ferrer; Vector Backbone:Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31253982
Comments: Please visit https://www.biorxiv.org/content/early/2018/08/27/400291 for bioRxiv preprint.
Note: Plasmid contains a N1396S mutation in dCas9. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_118171 Copy
Species: Homo sapiens
Genetic Insert: KRAB-dCas9-2A-BlastR
Vector Backbone Description: Backbone Marker:Jorge Ferrer; Vector Backbone:Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31253982
Comments: Please visit https://www.biorxiv.org/content/early/2018/08/27/400291 for bioRxiv preprint.
Note: Plasmid contains a N1396S mutation in dCas9. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_118170 Copy
Species: Homo sapiens
Genetic Insert: KRAB-dCas9-P2A-BlastR
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene #52961); Vector Backbone:lentiCRISPRv2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31253982
Comments: We have also generated a version of this backbone with the core EF1a promoter driving the expression of KRAB-dCas9. We advise users to test both backbones in their cell type of interest. In our experience this backbone yields better knockdown in liver and pancreatic beta cells. Please visit https://www.biorxiv.org/content/early/2018/08/27/400291 for bioRxiv preprint.
We have also made available plasmids to be used as negative controls, which contain non-human genome target sequences (BFP or EGFP). Please see Addgene #118161, #118162, #118163 and #118164.
There is a third BsmBI site in the promoter region, which does not have an impact on the GoldenGate cloning. We recommend using the provided cloning protocol.
Note: Plasmid contains a N1396S mutation in dCas9. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_118155 Copy
Species: Homo sapiens
Genetic Insert: KRAB-dCas9-P2A-BlastR
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene #52961); Vector Backbone:lentiCRISPRv2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Comments: We have also generated a version of this backbone with the hPGK promoter driving the expression of KRAB-dCas9. We advise users to test both backbones in their cell type of interest.
Note: Plasmid contains a N1396S mutation in dCas9. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_118154 Copy
Species: Homo sapiens
Genetic Insert: KRAB-dCas9-2A-BlastR
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene #52961); Vector Backbone:Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31253982
Comments: This plasmid is intended to be used as negative control for experiments using gRNAs cloned into Addgene Plasmid #118155
Please visit https://www.biorxiv.org/content/early/2018/08/27/400291 for bioRxiv preprint.
Note: Plasmid contains a N1396S mutation in dCas9. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_118162 Copy
Species: Homo sapiens
Genetic Insert: KRAB-dCas9-2A-BlastR
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene #52961); Vector Backbone:Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31253982
Comments: This plasmid is intended to be used as negative control for experiments using gRNAs cloned into Addgene Plasmid #118155
Please visit https://www.biorxiv.org/content/early/2018/08/27/400291 for bioRxiv preprint.
Note: Plasmid contains a N1396S mutation in dCas9. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_118164 Copy
Species: Homo sapiens
Genetic Insert: htt 46Q
Vector Backbone Description: Backbone Size:0; Vector Backbone:p426 GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10677504
Comments: N terminal region of Ht with polyQ repeat length of 46 fused to GFP.
Proper citation: RRID:Addgene_1182 Copy
Species: Homo sapiens
Genetic Insert: Homo sapiens Sphingosine Kinase 2
Vector Backbone Description: Vector Backbone:pYES2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29672528
Proper citation: RRID:Addgene_118092 Copy
Species: Homo sapiens
Genetic Insert: Homo sapiens Sphingosine Kinase 1
Vector Backbone Description: Backbone Size:5800; Vector Backbone:pYES2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29672528
Proper citation: RRID:Addgene_118091 Copy
Species: Homo sapiens
Genetic Insert: Caspase-2 Delta Card
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET-23 b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_11810 Copy
Species: Homo sapiens
Genetic Insert: dCas9-VP64-2A-BlastR
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene #75112); Vector Backbone:lentiSAMv2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31253982
Comments: Please visit https://www.biorxiv.org/content/early/2018/08/27/400291 for bioRxiv preprint.
Proper citation: RRID:Addgene_118199 Copy
Species: Homo sapiens
Genetic Insert: dCas9-VP64-2A-BlastR
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene #75112); Vector Backbone:lentiSAMv2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31253982
Comments: Please visit https://www.biorxiv.org/content/early/2018/08/27/400291 for bioRxiv preprint.
Proper citation: RRID:Addgene_118198 Copy
Species: Homo sapiens
Genetic Insert: human EB1
Vector Backbone Description: Vector Backbone:pEF1α-IRES-EGFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: Please refer to pLenti-IFT20-EGFP for cloning info.
Proper citation: RRID:Addgene_118084 Copy
Species: Homo sapiens
Genetic Insert: Caspase 7
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9325297
Proper citation: RRID:Addgene_11815 Copy
Species: Homo sapiens
Genetic Insert: Caspase 8 catalytic mutant
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9325297
Proper citation: RRID:Addgene_11818 Copy
Species: Homo sapiens
Genetic Insert: Caspase 8
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9325297
Proper citation: RRID:Addgene_11817 Copy
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