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Species: Gallus gallus
Genetic Insert: Fz 7
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(B); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10862751
Comments: Frizzled 7 wild type ORF: 5’ BsaI-primer, 3’ EcoRI-primer,
shuttle vector: Slax13.
Proper citation: RRID:Addgene_13930 Copy
Species: Gallus gallus
Genetic Insert: Fz 7
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10862751
Comments: Frizzled 7 wild type ORF: 5’ BsaI-primer, 3’ EcoRI-primer,
shuttle vector: Slax13.
Proper citation: RRID:Addgene_13929 Copy
Species: Gallus gallus
Genetic Insert: pitx2
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9708733
Comments: full length original library isolate from Mackem dT, stage18-24 limb bud library. Used as an anti-sense riboprobe template and for subcloning.
Proper citation: RRID:Addgene_13927 Copy
Species: Gallus gallus
Genetic Insert: pitx2
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9708733
Comments: The entire open reading frame of cPitx2 was generated by high fidelity PCR and cloned into a version of pSlax13 containing a triple HA (Flu) tag (Laufer et al., 1997). The sequence of the PCR-generated insert was confirmed by sequencing. A ClaI fragment containing the cPitx2-Flu sequence was cloned into the RCAS retroviral vector.
Proper citation: RRID:Addgene_13928 Copy
Species: Gallus gallus
Genetic Insert: src K295R
Vector Backbone Description: Backbone Marker:BD Biosciences; Backbone Size:6600; Vector Backbone:pLNCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_13659 Copy
Species: Gallus gallus
Genetic Insert: Src
Vector Backbone Description: Backbone Marker:BD Biosciences; Backbone Size:6600; Vector Backbone:pLNCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_13665 Copy
Species: Gallus gallus
Genetic Insert: Occludin
Vector Backbone Description: Vector Backbone:pCB6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8769425
Proper citation: RRID:Addgene_136637 Copy
Species: Gallus gallus
Genetic Insert: OccludinCT3
Vector Backbone Description: Vector Backbone:pCB6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8769425
Proper citation: RRID:Addgene_136639 Copy
Species: Gallus gallus
Genetic Insert: HAOccludinCT3
Vector Backbone Description: Vector Backbone:pCB6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8769425
Comments: There exist S119N, Y120A, and M214N in HAOccludinCT3 that are not known to impact the function of the plasmid as published.
Proper citation: RRID:Addgene_136640 Copy
Species: Gallus gallus
Genetic Insert: enhNC1.1_M3-TF_MCS-2A-eGFP-SV40pA
Vector Backbone Description: Backbone Size:4979; Vector Backbone:pTK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Contains EcoRV restriction enzyme site for cloning protein of interest.
Proper citation: RRID:Addgene_110203 Copy
Species: Gallus gallus
Genetic Insert: vinculin
Vector Backbone Description: Backbone Marker:modified pEGFP-C1; Vector Backbone:pmKikGRNB; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22328520
Comments: pmKikGR was constructed by inserting sequence corresponding to mKikGR with Kozak sequence to replace EGFP in Nhe1 /BglII sites of pEGFP NBC1. Vinculin cDNA was from Dr. Benjamin Geiger (Weizmann Institute) and mKikGR was from Dr. Atsushi Miyawaki (Riken BSI).
Proper citation: RRID:Addgene_105316 Copy
Species: Gallus gallus
Genetic Insert: Clover-mRuby2-based vinculin tension sensor with (GGSGGS)5 extensible domain
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5399; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30024378
Comments: Partially based on Addgene Plasmids #26019, #49089, and #21769
Proper citation: RRID:Addgene_111763 Copy
Species: Gallus gallus
Genetic Insert: Clover-mRuby2-based vinculin tension sensor with (GGSGGS)9 extensible domain
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5399; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30024378
Comments: Partially based on Addgene Plasmids #26019, #49089, and #21769
Proper citation: RRID:Addgene_111765 Copy
Species: Gallus gallus
Genetic Insert: VinculinTS (A50I)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5402; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29642037
Proper citation: RRID:Addgene_111827 Copy
Species: Gallus gallus
Genetic Insert: VinculinTS (I997A)
Vector Backbone Description: Backbone Size:6235; Vector Backbone:pRRL; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29642037
Proper citation: RRID:Addgene_111832 Copy
Species: Gallus gallus
Genetic Insert: VinculinTS (A50I)
Vector Backbone Description: Backbone Size:6235; Vector Backbone:pRRL; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29642037
Proper citation: RRID:Addgene_111831 Copy
Species: Gallus gallus
Genetic Insert: ednrbE2
Vector Backbone Description: Vector Backbone:pTK; Vector Types:Enhancer reporter construct; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31792380
Proper citation: RRID:Addgene_127771 Copy
Species: Gallus gallus
Genetic Insert: ednrbE2
Vector Backbone Description: Vector Backbone:pTK; Vector Types:Enhancer reporter construct; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31792380
Proper citation: RRID:Addgene_127770 Copy
Species: Gallus gallus
Genetic Insert: sox10
Vector Backbone Description: Vector Backbone:pTK; Vector Types:Enhancer reporter construct; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31792380
Proper citation: RRID:Addgene_127775 Copy
Species: Gallus gallus
Genetic Insert: ednrbE1
Vector Backbone Description: Vector Backbone:pTK; Vector Types:Enhancer reporter construct; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31792380
Proper citation: RRID:Addgene_127773 Copy
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