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Vector Backbone Description: Backbone Marker:NOVAGEN; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23345122
Proper citation: RRID:Addgene_42606 Copy
Species: Rattus norvegicus
Genetic Insert: ankyrin G
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4000; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21551097
Proper citation: RRID:Addgene_42566 Copy
Genetic Insert: attL1::egfp (870 bp)::attL2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4762; Vector Backbone:pDONR 221; Vector Types:Multi-site Gateway donor plasmid; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_42930 Copy
Species: Homo sapiens
Genetic Insert: NKX3.1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET-30a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19780584
Comments: This is a full length NKX3.1 cDNA construct. Protein expressed in E.coli BL21(DE3) competent cells
Proper citation: RRID:Addgene_42896 Copy
Species: Strongyloides stercoralis
Genetic Insert: attR2::Ss-era-1 terminator (598 bp)::attL3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4773; Vector Backbone:pDONR P2R-P3; Vector Types:Multi-site Gateway donor plasmid; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_42928 Copy
Species: A. victoria
Genetic Insert: Aquamarine
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23192565
Proper citation: RRID:Addgene_42888 Copy
Genetic Insert: CRISPR-BsaI
Vector Backbone Description: Backbone Size:2254; Vector Backbone:pZE21-MCS1; Vector Types:Bacterial Expression, CRISPR, E.coli; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23360965
Comments: This plasmid is also known as pDB129.
This plasmid contains a duplicated spacer insertion site (4 BsaI sites instead of 2). Please note that these extra sites are eliminated upon treatment with BsaI, and the depositing lab has used this version extensively without issue. Addgene has found that versions of the plasmid with and without the duplicated spacer are present, both are predicted to perform similarly.
The HME63 strain is available from the from the Court Lab:
http://redrecombineering.ncifcrf.gov/strains.html
For more information on Marraffini Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/marraffini/
Proper citation: RRID:Addgene_42875 Copy
Species: Perch (Morone americana)
Genetic Insert: Connexin 35 C-terminal domain
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5930; Vector Backbone:pET42c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15857663
Comments: Stop codon at CamKII phosphorylation site near tip of the C-terminus. Truncates last 7 amino acids.
Proper citation: RRID:Addgene_42913 Copy
Species: Mus musculus
Genetic Insert: PECAM
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pcDNAI Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Compared to GenBank accession number L06039.1, the PECAM-1 sequence in this plasmid contains 2 nucleotide mismatches in the 5' untranslated region (UTR).
Proper citation: RRID:Addgene_42901 Copy
Species: Homo sapiens
Genetic Insert: PECAM
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pcDNAI Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_42902 Copy
Species: Homo sapiens
Genetic Insert: Cry2PHR-mCherry-RhoA
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3991; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23377377
Proper citation: RRID:Addgene_42958 Copy
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22245580
Comments: NOTE: there is a second, non-annotated EcoRI site present in this plasmid. The function of the plasmid will not be impaired; however,
you cannot use the EcoRI site in the MCS for cloning.
Proper citation: RRID:Addgene_43796 Copy
Vector Backbone Description: Backbone Marker:Novagen, EMBL; Backbone Size:6841; Vector Backbone:pET, pETM44; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Proper citation: RRID:Addgene_43986 Copy
Vector Backbone Description: Backbone Marker:Novagen, EMBL; Backbone Size:7237; Vector Backbone:pET, pETM14; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Proper citation: RRID:Addgene_43987 Copy
Vector Backbone Description: Backbone Marker:Novagen, EMBL; Backbone Size:6660; Vector Backbone:pET, pET28M-Sumo1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Proper citation: RRID:Addgene_43989 Copy
Vector Backbone Description: Backbone Marker:Novagen,; Backbone Size:6091; Vector Backbone:pET, pCoofy2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Proper citation: RRID:Addgene_43982 Copy
Vector Backbone Description: Backbone Marker:Novagen, EMBL; Backbone Size:6406; Vector Backbone:pET, pETM33; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Note that the 6x His GST double tag tends to increase the formation of insoluble protein aggregates.
Proper citation: RRID:Addgene_43983 Copy
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5749; Vector Backbone:pET, pCoofy1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Note that depending on the protein, the 10x His tag can reduce or abolish expression.
Proper citation: RRID:Addgene_43975 Copy
Vector Backbone Description: Backbone Marker:Novagen, EMBL; Backbone Size:5752; Vector Backbone:pET, pETM14; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Proper citation: RRID:Addgene_43976 Copy
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5809; Vector Backbone:pET, pCoofy1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Proper citation: RRID:Addgene_43977 Copy
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