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Vector Backbone Description: Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30185429
Comments: Clone cDNA into vector using a Gateway LR reaction. Transform verified plasmid into an expression strain (e.g. BL21(DE3)pLysS) for recombinant protein production.
Proper citation: RRID:Addgene_139787 Copy
Species: Synthetic
Genetic Insert: mRFP-T2A-BlasticidinR
Vector Backbone Description: Vector Backbone:modified PB-CA (Addgene 20960) ; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31959800
Proper citation: RRID:Addgene_137881 Copy
Genetic Insert: Cherry
Vector Backbone Description: Backbone Size:5778; Vector Backbone:na; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Comments: There is a H30R mutation in mCherry. This mutation does not affect plasmid function and the construct has been validated for expression.
Proper citation: RRID:Addgene_13075 Copy
Vector Backbone Description: Backbone Size:4723; Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31541098
Proper citation: RRID:Addgene_134979 Copy
Vector Backbone Description: Backbone Size:5288; Vector Backbone:pOO2-GW; Vector Types:bacterial expression vector for generating SP6 transcripts in vitro for Xenopus oocyte transfection; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21107422
Comments: The AtAMT1;1 coding sequence with the att sites (attB1 and attB2) from the pDRf1-AtAMT1;1 (Loque et al 2007 Nature 446 195-198) was remobilized with both restriction enzymes SpeI and XhoI then subsequently cloned between SpeI and XhoI sites in the p6013-002 (Ludewig et al., 2002 J. Biol. Chem. 277, 13548-13555). The AtAMT1;1 coding sequence was then replaced by the Gateway DNA cassette after a BP reaction with pDONR 221 (GATEWAY technology, Invitrogen) to generate the p002-GW vector.
Proper citation: RRID:Addgene_135097 Copy
Vector Backbone Description: Backbone Size:8000; Vector Backbone:pMH-EF1-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:32611815
Proper citation: RRID:Addgene_133018 Copy
Vector Backbone Description: Backbone Size:7000; Vector Backbone:pMH-CMV-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:32611815
Proper citation: RRID:Addgene_133017 Copy
Genetic Insert: HIS3 reporter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pDEST6; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:16777607
Comments: Please note that the ccdB cassette in the author's map (click on 'View map') is shown in the reverse orientation.
Y1H Destination vector that can be used in conventional Gateway LR cloning reactions. This vector contains a Gateway cassette with AttR4 and AttL1 recombination sites and a HIS3 reporter gene. For more information and protocols, see Deplancke et al. 2004 Genome Res 14(10B):2093 and Deplancke et al. 2006 CSH Protocols.
Proper citation: RRID:Addgene_13349 Copy
Vector Backbone Description: Backbone Size:9709; Vector Backbone:pAW; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30217970
Proper citation: RRID:Addgene_133893 Copy
Vector Backbone Description: Vector Backbone:pUC57-R1R4; Vector Types:Other; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23066669
Proper citation: RRID:Addgene_105110 Copy
Vector Backbone Description: Vector Backbone:pXN21-Dest; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18428659
Proper citation: RRID:Addgene_105086 Copy
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:7934; Vector Backbone:pET19b; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18064608
Comments: pET19b Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate an expression clone containing an N-terminal His tag. Note that the Addgene verified sequence detects a mutation in the enterokinase site D(V)DDK.
Proper citation: RRID:Addgene_18668 Copy
Genetic Insert: Gateway cassette A
Vector Backbone Description: Backbone Size:4750; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:16904174
Comments: The sequence of primer pMX-S1811 is GAC GGC ATC GCA GCT TGG ATA CAC.
Proper citation: RRID:Addgene_18656 Copy
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:6932; Vector Backbone:pFlag-CMV-2; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18064608
Comments: pFLAG-CMV-2 Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate an expression clone containing an N-terminal FLAG tag.
Proper citation: RRID:Addgene_18700 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7755; Vector Backbone:pcDNA3.1(-)/Myc-His B; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18064608
Comments: pcDNA3.1(-)/myc-His B Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate an expression clone containing an C-terminal myc-His tag.
Proper citation: RRID:Addgene_18701 Copy
Vector Backbone Description: Backbone Size:8718; Vector Backbone:pJG4-5; Vector Types:Yeast Expression, two-hybrid prey vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18064608
Comments: pJG4-5 Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate a prey vector expression clone for yeast two-hybrid.
Proper citation: RRID:Addgene_18704 Copy
Vector Backbone Description: Backbone Size:12414; Vector Backbone:pEG202; Vector Types:Yeast Expression, two-hybrid bait vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18064608
Comments: pEG202 Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate a bait vector expression clone for yeast two-hybrid.
Proper citation: RRID:Addgene_18705 Copy
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pRSET; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18522753
Comments: pGWF1 s a Destination Vector. It carries the lethal ccdB gene (The ccdB gene is replaced with your gene of interest during the LR reaction). The Destination Vectors will only grow on ccdB resistant cells from Invitrogen, Catalog no. A10460.
Proper citation: RRID:Addgene_18856 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R1-R2)
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19635 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R1-R2)
Defining Citation: PMID:18723890
Comments: Sequence mismatches between Addgene sequence and depositor sequence do not affect function.
Proper citation: RRID:Addgene_19637 Copy
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