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Species: E. coli
Genetic Insert: Gro EL/ES
Vector Backbone Description: Backbone Marker:EMD Millipore; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23220215
Proper citation: RRID:Addgene_83923 Copy
Species: E. coli
Genetic Insert: SCRIBE_kanR(ON)
Vector Backbone Description: Backbone Marker:Expressys; Vector Backbone:pZE32; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25395541
Proper citation: RRID:Addgene_61450 Copy
Species: E. coli
Genetic Insert: Beta recombinase
Vector Backbone Description: Backbone Marker:Expressys; Vector Backbone:pZA11; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25395541
Proper citation: RRID:Addgene_61446 Copy
Species: E. coli
Genetic Insert: nptII gene
Vector Backbone Description: Backbone Marker:CAMBIA; Backbone Size:6812; Vector Backbone:pCAMBIA-0380; Vector Types:Ternary vector for Agrobacterium mediated transformation of fungi; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26092796
Comments: Jason Rudd, (Rothamsted research UK) provided Agrobacterium tumfaciens vector pCGEN (pCAMBIA-0380 backbone with nptII gene under Neurospora crassa gpd promoter).
NptII (neomycin phosphotransferase II) gene from pCGEN was recombined into pC-HYG-YR (Addgene plasmid# 61765) through yeast recombineering to replace hygromycin gene and create pC-G418-YR.
Restriction sites EcoRI and HindIII can be used to linearise the vector inside left (LB) and right (RB) borders of the TDNA. Note that the trpC promoter and nptII sequences are present within the EcoRI and HindII cloning sites.
See Supplementary Fig. S1. of associated publication for schematic of single step assembly of gene deletion ternary vectors using yeast recombinational cloning.
Please note that the Addgene verified sequence identified a transposase in the backbone (7145-8353 bp).
Proper citation: RRID:Addgene_61767 Copy
Species: E. coli
Genetic Insert: lacZ alpha
Vector Backbone Description: Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_61972 Copy
Species: E. coli
Genetic Insert: lacZ alpha
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_61973 Copy
Species: E. coli
Genetic Insert: lacZ alpha
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_61974 Copy
Species: E. coli
Genetic Insert: Thr251Gly-EcPheRS
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:4541; Vector Backbone:pQE-80L-Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25691744
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_62598 Copy
Species: E. coli
Genetic Insert: Encodes the hybrid receptor of E. coli chemoreceptor Tar and histidine kinase CitA: CitA[1-193]-Tar[204-553]
Vector Backbone Description: Backbone Size:4211; Vector Backbone:pKG116; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27285081
Proper citation: RRID:Addgene_82037 Copy
Species: E. coli
Genetic Insert: Encodes the hybrid receptor of E. coli chemoreceptor Tar and histidine kinase NarQ: NarQ[1-217]-Tar[257-553]
Vector Backbone Description: Backbone Size:4211; Vector Backbone:pKG116; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27285081
Proper citation: RRID:Addgene_82040 Copy
Species: E. coli
Genetic Insert: Eco1: RT and ncRNA(extended), a1/a2 length: 27
Vector Backbone Description: Vector Backbone:pZS165 (Addgene plasmid #114451); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184965 Copy
Species: E. coli
Genetic Insert: Eco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 12
Vector Backbone Description: Vector Backbone:pZS165 (Addgene plasmid #114451); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184972 Copy
Species: E. coli
Genetic Insert: Eco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v1
Vector Backbone Description: Vector Backbone:pZS165 (Addgene plasmid #114451); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184973 Copy
Species: E. coli
Genetic Insert: Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 12
Vector Backbone Description: Vector Backbone:pET-21(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184956 Copy
Species: E. coli
Genetic Insert: Eco2: RT and ncRNA(wt), a1/a2 length: 13
Vector Backbone Description: Vector Backbone:PiggyBac; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184992 Copy
Species: E. coli
Genetic Insert: Eco1: HEK4 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
Vector Backbone Description: Vector Backbone:pFUGW-H1 (Addgene plasmid #25870); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_185000 Copy
Species: E. coli
Genetic Insert: Eco1: FANCF targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
Vector Backbone Description: Vector Backbone:pFUGW-H1 (Addgene plasmid #25870); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184999 Copy
Species: E. coli
Genetic Insert: Eco2: RT and ncRNA(extended), a1/a2 length: 29
Vector Backbone Description: Vector Backbone:pZS165 (Addgene plasmid #114451); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184968 Copy
Species: E. coli
Genetic Insert: Eco1: RT and ncRNA(wt), a1/a2 length: 12
Vector Backbone Description: Vector Backbone:pZS165 (Addgene plasmid #114451); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184964 Copy
Species: E. coli
Genetic Insert: Eco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 22
Vector Backbone Description: Vector Backbone:pET-21(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184963 Copy
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