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Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 Spike protein
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:2900; Vector Backbone:pRSET5A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:35123472
Proper citation: RRID:Addgene_171639 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 S protein
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33243994
Comments: Pseudotypes to the MLV vector but not efficiently to VSV or lentiviral vectors.
Please visit https://www.biorxiv.org/content/10.1101/2020.06.12.148726v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_159364 Copy
Species: SARS-CoV-2
Genetic Insert: N-terminal His tag PreScission SARS-CoV-2 nsp13, optimized for insect cell expression
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5297; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32783916
Comments: Protein sequence was optimized by GenScript for insect cell expression using the OptimumGene Codon Optimization Analysis.
Please visit https://doi.org/10.1101/2020.07.08.194084 for bioRxiv preprint.
Proper citation: RRID:Addgene_159390 Copy
Species: SARS-CoV-2
Genetic Insert: SpyTag-SARS-CoV-2 RBD (340-538)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32886840
Proper citation: RRID:Addgene_159999 Copy
Species: SARS-CoV-2
Genetic Insert: nsp8
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pET46; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34041264
Proper citation: RRID:Addgene_164578 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 soluble spike
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32991842
Proper citation: RRID:Addgene_164651 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 soluble spike
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32991842
Proper citation: RRID:Addgene_164652 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 FSE
Vector Backbone Description: Vector Backbone:pUbC; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34185680
Proper citation: RRID:Addgene_177620 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 FSE
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pmirGLO; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34185680
Proper citation: RRID:Addgene_177615 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 FSE
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pmirGLO; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34185680
Proper citation: RRID:Addgene_177617 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 FSE
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pmirGLO; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34185680
Proper citation: RRID:Addgene_177616 Copy
Species: SARS-CoV-2
Genetic Insert: Spike (Lambda variant)
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.3; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34519517
Proper citation: RRID:Addgene_177579 Copy
Species: SARS-CoV-2
Genetic Insert: 43740568
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35857439
Comments: Read the preprint on bioRxiv: https://www.biorxiv.org/content/10.1101/2022.04.11.487879v1.
Proper citation: RRID:Addgene_186810 Copy
Species: SARS-CoV-2
Genetic Insert: Spike (XBB.1.5)
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_196585 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 N protein
Vector Backbone Description: Backbone Size:5427; Vector Backbone:pcDNA 3.1 BS (-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35296517
Proper citation: RRID:Addgene_188448 Copy
Species: SARS-CoV-2
Genetic Insert: express SARS2-NSP1-trunc
Vector Backbone Description: Vector Backbone:pVPSB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33188728
Proper citation: RRID:Addgene_192172 Copy
Species: SARS-CoV-2
Genetic Insert: Spike (BA.2.75.2)
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_194498 Copy
Species: SARS-CoV-2
Genetic Insert: Spike (XBB)
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36889306
Comments: Preprint: https://www.biorxiv.org/content/10.1101/2022.03.04.479488v1
Proper citation: RRID:Addgene_194494 Copy
Species: SARS-CoV-2
Genetic Insert: RBD
Vector Backbone Description: Vector Backbone:pExp-His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37328472
Comments: LIC cloning used 5' site BsaI (destroyed in cloning) and 3' site HindIII (not destroyed).
Proper citation: RRID:Addgene_194999 Copy
Species: SARS-CoV-2
Genetic Insert: RBD
Vector Backbone Description: Vector Backbone:pOP2H; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39143416
Comments: LIC cloning used 5' site NcoI (destroyed in cloning) and 3' site XhoI (not destroyed). Please visit https://doi.org/10.1101/2023.01.10.523494 for bioRxiv preprint.
Proper citation: RRID:Addgene_195002 Copy
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