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Vector Backbone Description: Vector Backbone:p3E; Vector Types:RNAi; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_163380 Copy
Species: Synthetic
Genetic Insert: codon-optimized iCre sequence
Vector Backbone Description: Vector Backbone:pME; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37601640
Proper citation: RRID:Addgene_171792 Copy
Species: Homo sapiens
Genetic Insert: HsDCP1a_1-132
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_147089 Copy
Species: Homo sapiens
Genetic Insert: HsDCP1a_132-582
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_147090 Copy
Vector Backbone Description: Backbone Size:7072; Vector Backbone:R4-R2 Gateway Destination clone for Tol2kit reaction (GFP under Crystallin promoter marker); Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:37601640
Proper citation: RRID:Addgene_171795 Copy
Species: Homo sapiens
Genetic Insert: VHL
Vector Backbone Description: Backbone Marker:Gift from V. Koduri; Vector Backbone:pLX304-gate-IRES-Tdtomato; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31575731
Proper citation: RRID:Addgene_187643 Copy
Species: Synthetic
Genetic Insert: Luciferase
Vector Backbone Description: Backbone Size:6368; Vector Backbone:pSBtet; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36292564
Proper citation: RRID:Addgene_194328 Copy
Species: Streptococcus thermophilus
Genetic Insert: FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; Puro
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36690762
Proper citation: RRID:Addgene_195240 Copy
Species: Homo sapiens
Genetic Insert: RON receptor tyrosine kinase
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1-V5/His; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28199182
Proper citation: RRID:Addgene_201983 Copy
Species: Homo sapiens
Genetic Insert: PDGFRA receptor tyrosine kinase
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1-V5/His; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28199182
Proper citation: RRID:Addgene_201987 Copy
Species: Synthetic
Genetic Insert: Synthetic construct isolate AAV-PHP.eC VP1 gene
Vector Backbone Description: Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37075114
Comments: Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. This work supported by NIH BRAIN Initiative grant UF1MH128336. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction.
Proper citation: RRID:Addgene_201903 Copy
Species: Homo sapiens
Genetic Insert: BRAF catalytic (CAT) domain (AA 435-766)
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36347258
Proper citation: RRID:Addgene_194772 Copy
Species: Mus musculus
Genetic Insert: Mus musculus EZR(1-586):EGFP
Vector Backbone Description: Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36416724
Proper citation: RRID:Addgene_202684 Copy
Species: Discosoma sp.
Genetic Insert: tdTomato
Vector Backbone Description: Backbone Size:5611; Vector Backbone:pAAV-EF1a-DIO; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37828037
Comments: Please visit https://doi.org/10.1101/2022.08.29.505018 for bioRxiv preprint.
Proper citation: RRID:Addgene_202610 Copy
Species: Synthetic
Genetic Insert: monomeric nuclear Tomato
Vector Backbone Description: Backbone Size:8000; Vector Backbone:pLV; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Our lab uses DH5alpha cells for growth, and recombination is generally not a problem with this lenti backbone. Use NEBstable or similar strains if recombination observed. Please visit https://www.biorxiv.org/content/10.1101/2023.11.21.568191v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_172335 Copy
Species: Synthetic
Genetic Insert: monomeric nuclear Tomato
Vector Backbone Description: Backbone Size:8000; Vector Backbone:pLV; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Our lab uses DH5alpha cells for growth, and recombination is generally not a problem with this lenti backbone. Use NEBstable or similar strains if recombination observed. Please visit https://www.biorxiv.org/content/10.1101/2023.11.21.568191v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_172328 Copy
Species: Homo sapiens
Genetic Insert: eEF1A1
Vector Backbone Description: Vector Backbone:pLX304; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36638793
Proper citation: RRID:Addgene_198383 Copy
Species: Homo sapiens
Genetic Insert: EGFP:PIP5K1A
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37534432
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.06.30.498262v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_202720 Copy
Species: Synthetic
Genetic Insert: Flag-GFP-TurboID-NES
Vector Backbone Description: Vector Backbone:pLX304; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37385249
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.02.07.527548v1 for bioRxiv preprint
Proper citation: RRID:Addgene_202019 Copy
Species: Synthetic
Genetic Insert: APEX2-V5-G3BP1
Vector Backbone Description: Vector Backbone:pTRE; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37385249
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.02.07.527548v1 for bioRxiv preprint
Proper citation: RRID:Addgene_202013 Copy
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