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Species: Saccharomyces cerevisiae
Genetic Insert: Mitochondrial FAD-linked sulfhydryl oxidase
Vector Backbone Description: Backbone Size:5068; Vector Backbone:pACYC184-TU2A-RFP; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.08.31.458447v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_176405 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ste2Sc
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: The gene was codon-optimized for expression in S. cervisiae and cloned into the vector using Goden Gate. The plasmid is Golden Gate Cloning compatible. Please visit https://doi.org/10.1101/2021.09.27.462023 for bioRxiv preprint.
Proper citation: RRID:Addgene_176731 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: P-PRM1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: The part was cloned into the vector using Goden Gate. The plasmid is Golden Gate Cloning compatible. Please visit https://doi.org/10.1101/2021.09.27.462023 for bioRxiv preprint.
Proper citation: RRID:Addgene_176709 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: P-MSG5
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: The part was cloned into the vector using Goden Gate. The plasmid is Golden Gate Cloning compatible. Please visit https://doi.org/10.1101/2021.09.27.462023 for bioRxiv preprint.
Proper citation: RRID:Addgene_176708 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VAC8
Vector Backbone Description: Vector Backbone:pRS405; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32508216
Comments: Linearize with AflII
Proper citation: RRID:Addgene_166838 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ATG5 ATG12 ATG16
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26812546
Proper citation: RRID:Addgene_166851 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Atg13[571–700]
Vector Backbone Description: Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31352862
Proper citation: RRID:Addgene_166855 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Atg13[571–700]
Vector Backbone Description: Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31352862
Proper citation: RRID:Addgene_166854 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Atg13[571–700]
Vector Backbone Description: Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31352862
Proper citation: RRID:Addgene_166852 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VAC8
Vector Backbone Description: Vector Backbone:pRS405; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32508216
Comments: Linearize with AflII
Proper citation: RRID:Addgene_166840 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VAC8
Vector Backbone Description: Vector Backbone:pRS405; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32508216
Comments: Linearize with AflII
Proper citation: RRID:Addgene_166844 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VAC8
Vector Backbone Description: Vector Backbone:pRS405; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32508216
Comments: Linearize with AflII
Proper citation: RRID:Addgene_166841 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ATG1
Vector Backbone Description: Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30527663
Comments: Linearize with StuI
Proper citation: RRID:Addgene_166847 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FCY-UPP fusion gene
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Synthetic Biology, MoClo Level 0 Vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33577114
Proper citation: RRID:Addgene_167301 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPA1
Vector Backbone Description: Vector Backbone:pET11d; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9730822
Comments: RPA1 5317 RPA2 7292 RPA3 8284 (start of coding region listed)
Proper citation: RRID:Addgene_102614 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: tryptophanyl tRNA synthetase
Vector Backbone Description: Vector Backbone:pRST11B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29120463
Proper citation: RRID:Addgene_102789 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cdc14/mCherry-AID(71-114)-NatMX
Vector Backbone Description: Backbone Marker:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC55758/; Backbone Size:2531; Vector Backbone:HO-poly-KanMX4-HO; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27989441
Comments: This plasmid can be used to dynamically and conditionally deplete Cdc14 in yeast.
A characterization guide can be found here:
https://www.nature.com/articles/s41598-017-04791-6
Detailed description of plasmid construction can be found in the Supplemental Experimental Procedures of the original paper:
http://www.cell.com/molecular-cell/pdfExtended/S1097-2765(16)30726-2
Proper citation: RRID:Addgene_102884 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: APEX2-NES
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:7500; Vector Backbone:pRS425; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27274088
Proper citation: RRID:Addgene_102951 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: bPAC
Vector Backbone Description: Vector Backbone:pNH605; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28035051
Proper citation: RRID:Addgene_103017 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: crPDR12-3.S
Vector Backbone Description: Backbone Marker:Świat M... Daran-Lapujade PAS FnCpf1, a novel and efficient genome editing tool for Saccharomyces cerevisiae. NAR; Backbone Size:4995; Vector Backbone:pUD628; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29106617
Proper citation: RRID:Addgene_103023 Copy
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