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Species: E. coli
Genetic Insert: Eco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v1
Vector Backbone Description: Vector Backbone:pZS165 (Addgene plasmid #114451); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184973 Copy
Species: E. coli
Genetic Insert: Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 12
Vector Backbone Description: Vector Backbone:pET-21(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184956 Copy
Species: E. coli
Genetic Insert: Eco2: RT and ncRNA(wt), a1/a2 length: 13
Vector Backbone Description: Vector Backbone:PiggyBac; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184992 Copy
Species: E. coli
Genetic Insert: Eco1: HEK4 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
Vector Backbone Description: Vector Backbone:pFUGW-H1 (Addgene plasmid #25870); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_185000 Copy
Species: E. coli
Genetic Insert: Eco1: FANCF targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
Vector Backbone Description: Vector Backbone:pFUGW-H1 (Addgene plasmid #25870); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184999 Copy
Species: E. coli
Genetic Insert: Eco2: RT and ncRNA(extended), a1/a2 length: 29
Vector Backbone Description: Vector Backbone:pZS165 (Addgene plasmid #114451); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184968 Copy
Species: E. coli
Genetic Insert: Eco1: RT and ncRNA(wt), a1/a2 length: 12
Vector Backbone Description: Vector Backbone:pZS165 (Addgene plasmid #114451); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184964 Copy
Species: E. coli
Genetic Insert: Eco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 22
Vector Backbone Description: Vector Backbone:pET-21(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184963 Copy
Species: E. coli
Genetic Insert: Eco1 editing ncRNA and gRNA, LYP1 E27X, a1/a2 length: 12
Vector Backbone Description: Vector Backbone:pZS165 (Addgene plasmid #114451); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184977 Copy
Species: E. coli
Genetic Insert: Eco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v2
Vector Backbone Description: Vector Backbone:pZS165 (Addgene plasmid #114451); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184974 Copy
Species: E. coli
Genetic Insert: Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 22
Vector Backbone Description: Vector Backbone:pET-21(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184957 Copy
Species: E. coli
Genetic Insert: Eco1: ncRNA(wt) and RT
Vector Backbone Description: Vector Backbone:pRSFDUET-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184953 Copy
Species: E. coli
Genetic Insert: Eco1 RT and recombineering ncRNA, fabH G954A, a1/a2 length: 22
Vector Backbone Description: Vector Backbone:pET-21(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184959 Copy
Species: E. coli
Genetic Insert: E. coli dihydrofolate reductase
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:5483; Vector Backbone:pcDNA DEST40; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36122928
Proper citation: RRID:Addgene_192815 Copy
Species: E. coli
Genetic Insert: 1-phosphofructokinase
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4600; Vector Backbone:pET3a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10833389
Proper citation: RRID:Addgene_186256 Copy
Species: E. coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Marker:PMID: 17878951; Backbone Size:8537; Vector Backbone:pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::B1-NLSLacZ-B2; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17878951
Comments: Gateway destination vector for electroporation experiments.
Proper citation: RRID:Addgene_186414 Copy
Species: E. coli
Genetic Insert: colicin E1 residues 1-190
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5343; Vector Backbone:pET303; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35199644
Comments: Please visit https://doi.org/10.1101/692251 for bioRxiv preprint.
Proper citation: RRID:Addgene_155180 Copy
Species: E. coli
Genetic Insert: colicin E1 residues 1-364
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5343; Vector Backbone:pET303; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35199644
Comments: Please visit https://doi.org/10.1101/692251 for bioRxiv preprint.
Proper citation: RRID:Addgene_155181 Copy
Species: E. coli
Genetic Insert: ribA
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5021; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35863619
Comments: Sequencing of pDL52 plasmids using primers binding the (repetitive) T7 promotor region is only possible after the separation of the two operons e.g. by restriction digestion with HindIII and NcoI followed by purification of the two fragments from preparative agarose gels.
Proper citation: RRID:Addgene_188578 Copy
Species: E. coli
Genetic Insert: ribA
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5021; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35863619
Comments: Sequencing of pDL52 plasmids using primers binding the (repetitive) T7 promotor region is only possible after the separation of the two operons e.g. by restriction digestion with HindIII and NcoI followed by purification of the two fragments from preparative agarose gels.
Proper citation: RRID:Addgene_188579 Copy
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