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Species: Saccharomyces cerevisiae
Genetic Insert: ConL2-pTDH3-mRUBY2-tADH1-ConRE AmpR-ColE1
Vector Backbone Description: Vector Backbone:NA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31788779
Proper citation: RRID:Addgene_129002 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: KanR-ColE1 URA 5'-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1--pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
Vector Backbone Description: Vector Backbone:NA; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31788779
Proper citation: RRID:Addgene_129008 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 5' URA3 homology-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-Spacer-URA3 URA3 3'-homology KanR-ColE1
Vector Backbone Description: Vector Backbone:NA; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31788779
Proper citation: RRID:Addgene_129009 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Rnq1
Vector Backbone Description: Backbone Marker:Szilvia Kiriakov; Vector Backbone:pSK247; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29056345
Comments: This is the Rnq1 dual yTRAP sensor plasmid to be used in combination with pSK260. pSK226 should be linearized with NotI-HF enzyme prior to integration into the yeast genome. The sensor integrates into the LEU2 locus, and can be selected for using 100 ug/ml G418.
Proper citation: RRID:Addgene_129268 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 N (nucleocapsid)
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009724397.2. Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145389 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 2'-O-ribose methyltransferase
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725311.1. Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145387 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 endoRNAse
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725310.1. Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145386 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 ORF3a protein
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009724391.1. Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145384 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 ORF7b
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725318.1. Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145382 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 ORF7b
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725318.1Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145437 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 ORF6 protein
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009724394.1Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145436 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 3C-like proteinase
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725301.1Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145434 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 nsp10
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725306.1Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145431 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 2'-O-ribose methyltransferase
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725311.1Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145449 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 nsp9
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725305.1Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145446 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 nsp10
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725306.1Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145447 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 nsp8
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725304.1Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145443 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 ORF7a protein
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009724395.1Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145440 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 leader protein
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725297.1Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145441 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 nsp11
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725312.1Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145457 Copy
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