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Species: Saccharomyces cerevisiae
Genetic Insert: RFC2
Vector Backbone Description: Vector Backbone:pET(11a) / pET(16b); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Mix & Match (co-transform) with other vectors to over-express Sc RFC complex or related complexes (with Sc RFC1 substituted by Sc rad24, Sc ctf18, or Sc Elg1) containing the desired mutation(s). Expression in CodonPlus(RIL) or other strain containing rare Sc tRNA genes recommended.
Proper citation: RRID:Addgene_258203 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RFC2
Vector Backbone Description: Vector Backbone:pET(11a) / pET(16b); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Mix & Match (co-transform) with other vectors to over-express Sc RFC complex or related complexes (with Sc RFC1 substituted by Sc rad24, Sc ctf18, or Sc Elg1) containing the desired mutation(s). Expression in CodonPlus(RIL) or other strain containing rare Sc tRNA genes recommended.
Proper citation: RRID:Addgene_258204 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RFC2
Vector Backbone Description: Vector Backbone:pET(11a) / pET(16b); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Mix & Match (co-transform) with other vectors to over-express Sc RFC complex or related complexes (with Sc RFC1 substituted by Sc rad24, Sc ctf18, or Sc Elg1) containing the desired mutation(s). Expression in CodonPlus(RIL) or other strain containing rare Sc tRNA genes recommended.
Proper citation: RRID:Addgene_258207 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RFC2
Vector Backbone Description: Vector Backbone:pET(11a) / pET(16b); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Mix & Match (co-transform) with other vectors to over-express Sc RFC complex or related complexes (with Sc RFC1 substituted by Sc rad24, Sc ctf18, or Sc Elg1) containing the desired mutation(s). Expression in CodonPlus(RIL) or other strain containing rare Sc tRNA genes recommended.
Proper citation: RRID:Addgene_258208 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ABEmax
Vector Backbone Description: Vector Backbone:pAG415; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41543903
Comments: Please visit https://doi.org/10.64898/2025.12.09.693232 for bioRxiv preprint.
Please note that the depositor's annotated reference file has minor differences compared to the Addgene verified sequence. The depositor confirms that G5A in EGFP in the Addgene sequence is expected and does not alter the plasmid's function. Please refer to the the Addgene sequence for the most accurate result.
Proper citation: RRID:Addgene_251965 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Myc-tagged Sed1
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_252977 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Rad51
Vector Backbone Description: Backbone Size:5857; Vector Backbone:pYES2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20864034
Proper citation: RRID:Addgene_33231 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PYK2
Vector Backbone Description: Backbone Marker:ATCC#87362 (PMID:7737504); Backbone Size:5557; Vector Backbone:P413TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21907146
Proper citation: RRID:Addgene_34606 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Mid2(SS/TM)-GFP-LOVpep
Vector Backbone Description: Backbone Size:4263; Vector Backbone:YIPlac128; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388287
Comments: The LOV2 domain is amino acids 404-540 and differs from the wild-type A. sativa LOV2 sequence by the point mutations G528A and N534E. See Strickland et al. for details.
Proper citation: RRID:Addgene_34967 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Mid2(SS/TM)-GFP-LOVpep
Vector Backbone Description: Backbone Size:4263; Vector Backbone:YIPlac128; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388287
Comments: The LOV2 domain is amino acids 404-540 and differs from the wild-type A. sativa LOV2 sequence by the point mutations G528A and N534E. See Strickland et al. for details.
Proper citation: RRID:Addgene_34966 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: cpPDZ–mCherry
Vector Backbone Description: Vector Backbone:YIPlac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388287
Proper citation: RRID:Addgene_34983 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ste11
Vector Backbone Description: Vector Backbone:pDS190; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388287
Proper citation: RRID:Addgene_34982 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: cpPDZ
Vector Backbone Description: Vector Backbone:YIPlac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388287
Proper citation: RRID:Addgene_34986 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ePDZb1
Vector Backbone Description: Vector Backbone:YIPlac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388287
Proper citation: RRID:Addgene_34985 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Mid2(SS/TM)-GFP-LOVpep
Vector Backbone Description: Backbone Size:4263; Vector Backbone:YIPlac128; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388287
Comments: The LOV2 domain is amino acids 404-540 and differs from the wild-type A. sativa LOV2 sequence by the point mutations G528A and N534E. See Strickland et al. for details.
Proper citation: RRID:Addgene_34972 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Mid2-GFP-LOVpep
Vector Backbone Description: Backbone Marker:EUROSCARF; Backbone Size:7747; Vector Backbone:pGREG505; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388287
Comments: The LOV2 domain is amino acids 404-540 and differs from the wild-type A. sativa LOV2 sequence by the point mutations G528A and N534E. See Strickland et al. for details.
Proper citation: RRID:Addgene_34975 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Mid2-GFP-LOVpep
Vector Backbone Description: Backbone Marker:EUROSCARF; Backbone Size:7747; Vector Backbone:pGREG505; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388287
Comments: The LOV2 domain is amino acids 404-540 and differs from the wild-type A. sativa LOV2 sequence by the point mutations G528A and N534E. See Strickland et al. for details.
Proper citation: RRID:Addgene_34974 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Mid2(SS/TM)-GFP-LOVpep
Vector Backbone Description: Backbone Size:4263; Vector Backbone:YIPlac128; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388287
Comments: The LOV2 domain is amino acids 404-540 and differs from the wild-type A. sativa LOV2 sequence by the point mutations G528A and N534E. See Strickland et al. for details.
Proper citation: RRID:Addgene_34973 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ePDZb
Vector Backbone Description: Backbone Marker:EUROSCARF; Backbone Size:6624; Vector Backbone:pGREG503; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388287
Proper citation: RRID:Addgene_34977 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Mid2(SS/TM)-GFP-LOVpep
Vector Backbone Description: Backbone Size:4263; Vector Backbone:YIPlac128; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388287
Comments: The LOV2 domain is amino acids 404-540 and differs from the wild-type A. sativa LOV2 sequence by the point mutations G528A and N534E. See Strickland et al. for details.
Proper citation: RRID:Addgene_34969 Copy
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