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Species: Escherichia coli
Genetic Insert: E. coli polyphosphate kinase
Vector Backbone Description: Backbone Marker:K. Melcher; Vector Backbone:pKM263; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_38333 Copy
Species: Escherichia coli
Genetic Insert: E. coli polyphosphatase
Vector Backbone Description: Backbone Marker:K. Melcher; Vector Backbone:pKM263; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17320430
Comments: See "Bolesch, D.G., Keasling, J.D., 2000. Polyphosphate binding and chain length recognition of Escherichia coli exopolyphosphatase. J. Biol. Chem. 275, 33814–33819" for more information.
Proper citation: RRID:Addgene_38328 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PPX1
Vector Backbone Description: Backbone Marker:K. Melcher; Vector Backbone:pKM263; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16184370
Proper citation: RRID:Addgene_38327 Copy
Species: Homo sapiens
Genetic Insert: ARRDC1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22315426
Proper citation: RRID:Addgene_38321 Copy
Species: Homo sapiens
Genetic Insert: Next to BRCA1 gene 1 protein
Vector Backbone Description: Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:5800; Vector Backbone:pMXs-IP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21220506
Comments: DNA res. 1, 223-229, (1994)
Proper citation: RRID:Addgene_38283 Copy
Species: Homo sapiens
Genetic Insert: YWHAG
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pTvHR21-SGC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: http://www.thesgc.org/structures/2B05/
Proper citation: RRID:Addgene_39129 Copy
Genetic Insert: none
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pFB-Sec-NH; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_39189 Copy
Species: Rattus norvegicus
Genetic Insert: Erk2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11352917
Proper citation: RRID:Addgene_39222 Copy
Species: Homo sapiens
Genetic Insert: INCENP
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pGTvL1-SGC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: http://www.thesgc.org/structures/3U23/
Proper citation: RRID:Addgene_39185 Copy
Species: Homo sapiens
Genetic Insert: p34
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7125; Vector Backbone:p2Bac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_39221 Copy
Genetic Insert: p44
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7125; Vector Backbone:p2Bac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_39220 Copy
Species: Homo sapiens
Genetic Insert: ERK2-MEK1 Fusion
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9799732
Comments: rat ERK2 fused to human MEK1 with a linker in between
Proper citation: RRID:Addgene_39194 Copy
Genetic Insert: none
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pFB-CT10HF-LIC; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin
Comments: Primers for LIC cloning:
Add the following 5’ extensions to the PCR primers:
Upstream: TTAAGAAGGAGATATACTATG (ATG-initiation codon)
Downstream: GATTGGAAGTAGAGGTTCTCTGC
The purified PCR fragments are treated with T4 DNA polymerase and dGTP, then annealed to the treated vector.
Proper citation: RRID:Addgene_39191 Copy
Species: Homo sapiens
Genetic Insert: p52
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7125; Vector Backbone:p2Bac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_39219 Copy
Species: Homo sapiens
Genetic Insert: XPG
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:6646; Vector Backbone:pMAL-c2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7657672
Proper citation: RRID:Addgene_39215 Copy
Species: Homo sapiens
Genetic Insert: ABL2
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pFB-LIC-Bse; Vector Types:Baculovirus Expression; Bacterial Resistance:Ampicillin
Comments: http://www.thesgc.org/structures/3GVU/
Proper citation: RRID:Addgene_39178 Copy
Species: Homo sapiens
Genetic Insert: BBOX1
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pFB-LIC-Bse; Vector Types:Baculovirus Expression; Bacterial Resistance:Ampicillin
Comments: http://www.thesgc.org/structures/3MS5/
Proper citation: RRID:Addgene_39176 Copy
Species: Homo sapiens
Genetic Insert: ROR2
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pFBOH-MHL; Vector Types:Baculovirus Expression; Bacterial Resistance:Ampicillin
Comments: http://www.thesgc.org/structures/3ZZW/
Proper citation: RRID:Addgene_39183 Copy
Species: Homo sapiens
Genetic Insert: ACSM2B
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pFB-LIC-Bse; Vector Types:Baculovirus Expression; Bacterial Resistance:Ampicillin
Comments: http://www.thesgc.org/structures/3C5E/
Proper citation: RRID:Addgene_39167 Copy
Species: Homo sapiens
Genetic Insert: NEK2
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: http://www.thesgc.org/structures/2JAV/
Proper citation: RRID:Addgene_39163 Copy
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