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  • Organism:saccharomyces cerevisiae (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pcDNA5/FRT/TO SSA1
 
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RRID:Addgene_19462 SSA1 Saccharomyces cerevisiae Ampicillin PMID:18686016 Family: HSP70 Disclaimer: For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene. Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:11:45 0
pcDNA5/FRT/TO HIS SSA1
 
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RRID:Addgene_19543 SSA1 Saccharomyces cerevisiae Ampicillin PMID:18686016 Part of the HSP70 gene family Disclaimer: For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene. Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:11:46 0
pRBUBR1
 
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RRID:Addgene_20198 UBR1 Saccharomyces cerevisiae Ampicillin PMID:18566452 Plasmid for overexpressing Ubr1ha in yeast. The ha tag is at the C-terminus of the protein and the protein is active in degrading N-end rule substrates. Backbone Size:5741; Vector Backbone:Yeplac 181; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:11:52 0
P2r-P3 Ste3pmCherry
 
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RRID:Addgene_58561 Ste3promoter-mCherry Saccharomyces cerevisiae Kanamycin Backbone Marker:Invitrogen; Vector Backbone:pDONR P2r-P3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin 2026-08-15 01:17:17 0
P4-P1r Ste2pCitrine
 
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RRID:Addgene_58560 Ste2 promoter - Citrine Saccharomyces cerevisiae Kanamycin Backbone Marker:Invitrogen; Vector Backbone:pDONR P4-P1r; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin 2026-08-15 01:17:17 0
pRS406-PHO5-GFP-2xOsh2 PH domain [short]
 
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1+ mentions
RRID:Addgene_58829 2xOsh2 PH domain [short] Saccharomyces cerevisiae Ampicillin PMID:15271978 This plasmid contains two copies of the short form of Osh2 PH domain (short form: Serine286-Lysine39) Backbone Marker:Stratagene; Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:17:19 1
pRS416-PHO5-GFP-Osh1
 
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RRID:Addgene_58835 Osh1 Saccharomyces cerevisiae Ampicillin PMID:12727870 Backbone Marker:Stratagene; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:17:19 0
pRS416-PHO5-GFP-Opi1 + terminator
 
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RRID:Addgene_58836 Opi1p Saccharomyces cerevisiae Ampicillin The plasmid includes the Opi1p gene's own terminator. Backbone Marker:Stratagene; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:17:19 0
pRS416-PHO5-GFP-Osh3
 
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1+ mentions
RRID:Addgene_58839 Osh3p Saccharomyces cerevisiae Ampicillin PMID:11408574 Backbone Marker:Stratagene; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:17:19 1
pEVA1
 
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RRID:Addgene_58953 VAR1u, VAR1 recoded for expression from the nucleus Saccharomyces cerevisiae Ampicillin PMID:9755179 Vector Backbone:Yep13; Vector Types:Yeast Expression, Yeast Ecoli shuttle vector; Bacterial Resistance:Ampicillin Synthetic gene in standard genetic code 2026-08-15 01:17:20 0
pJC30X-BS(X)
 
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RRID:Addgene_58958 Enhanced fluorescence variant of GFP, in S. cerevisiae mitochondrial genetic code Saccharomyces cerevisiae Ampicillin PMID:16120277 Backbone Size:2900; Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Ampicillin GFP modified as described: CORMACK, B. P., R. H. VALDIVIA and S. FALKOW, 1996 FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173: 33-38. 2026-08-15 01:17:20 0
HC078 (3xHA-Atg13)
 
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1+ mentions
RRID:Addgene_59544 ATG13 Saccharomyces cerevisiae Ampicillin PMID:18077553 Vector Backbone:pRS315; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin AvrII site created after start codon 2026-08-15 01:17:23 2
pNB813
 
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RRID:Addgene_60161 yEVenus-PEST Saccharomyces cerevisiae Ampicillin PMID:25232010 Backbone Size:6682; Vector Backbone:p405; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:17:29 0
pNB812
 
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RRID:Addgene_60160 yEVenus-PEST Saccharomyces cerevisiae Ampicillin PMID:25232010 Backbone Size:6682; Vector Backbone:p405; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:17:29 0
pNB776
 
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RRID:Addgene_60117 RdLuc Saccharomyces cerevisiae Ampicillin PMID:25232010 Backbone Marker:DNA 2.0; Backbone Size:2707; Vector Backbone:pJ204; Vector Types:Unspecified; Bacterial Resistance:Ampicillin 2026-08-15 01:17:29 0
Gal4pBS12-Pleum
 
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RRID:Addgene_60326 promoter Saccharomyces cerevisiae Ampicillin PMID:22565375 Backbone Size:6000; Vector Backbone:p416; Vector Types:Yeast Expression, Synthetic Biology, Yeast synthetic hybrid promoter construct; Bacterial Resistance:Ampicillin 2026-08-15 01:17:31 0
pRS424-TUB2 - human TUBB1 Cterminal tail
 
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RRID:Addgene_60400 TUB2 Saccharomyces cerevisiae Ampicillin PMID:24633327 C-terminal tail is from TUBB1 (tubulin, beta 1 class VI) according to HUGO nomenclature: http://www.genenames.org/genefamilies/TUB Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin TUB2 amino acids 429 - end, replaced with human TUBB1 sequence 2026-08-15 01:17:32 0
pRS424-TUB2 - human TUBB4a Cterminal tail C127S, E435C
 
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RRID:Addgene_60405 TUB2 Saccharomyces cerevisiae Ampicillin PMID:24633327 Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin TUB2 amino acids 429 - end, replaced with human TUBB4a sequence, C127S, E435C 2026-08-15 01:17:32 0
pRS424-TUB2 - human TUBB3 Cterminal tail delta K
 
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RRID:Addgene_60404 TUB2 Saccharomyces cerevisiae Ampicillin PMID:24633327 Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin TUB2 amino acids 429 - end, replaced with human TUBB3 sequence without last 4 amino acids (QGPK) 2026-08-15 01:17:32 0
pRS424-TUB2 - human TUBB4a Cterminal tail
 
Resource Report
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RRID:Addgene_60402 TUB2 Saccharomyces cerevisiae Ampicillin PMID:24633327 Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin TUB2 amino acids 429 - end, replaced with human TUBB4a sequence 2026-08-15 01:17:32 0

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