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Species: Saccharomyces cerevisiae
Genetic Insert: SSA1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18686016
Comments: Family: HSP70
Disclaimer:
For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene.
Proper citation: RRID:Addgene_19462 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SSA1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18686016
Comments: Part of the HSP70 gene family
Disclaimer:
For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene.
Proper citation: RRID:Addgene_19543 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: UBR1
Vector Backbone Description: Backbone Size:5741; Vector Backbone:Yeplac 181; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18566452
Comments: Plasmid for overexpressing Ubr1ha in yeast. The ha tag is at the C-terminus of the protein and the protein is active in degrading N-end rule substrates.
Proper citation: RRID:Addgene_20198 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ste3promoter-mCherry
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR P2r-P3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_58561 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ste2 promoter - Citrine
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR P4-P1r; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_58560 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 2xOsh2 PH domain [short]
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15271978
Comments: This plasmid contains two copies of the short form of Osh2 PH domain (short form: Serine286-Lysine39)
Proper citation: RRID:Addgene_58829 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Osh1
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12727870
Proper citation: RRID:Addgene_58835 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Opi1p
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: The plasmid includes the Opi1p gene's own terminator.
Proper citation: RRID:Addgene_58836 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Osh3p
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11408574
Proper citation: RRID:Addgene_58839 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VAR1u, VAR1 recoded for expression from the nucleus
Vector Backbone Description: Vector Backbone:Yep13; Vector Types:Yeast Expression, Yeast Ecoli shuttle vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9755179
Proper citation: RRID:Addgene_58953 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Enhanced fluorescence variant of GFP, in S. cerevisiae mitochondrial genetic code
Vector Backbone Description: Backbone Size:2900; Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16120277
Proper citation: RRID:Addgene_58958 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ATG13
Vector Backbone Description: Vector Backbone:pRS315; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18077553
Proper citation: RRID:Addgene_59544 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: yEVenus-PEST
Vector Backbone Description: Backbone Size:6682; Vector Backbone:p405; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25232010
Proper citation: RRID:Addgene_60161 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: yEVenus-PEST
Vector Backbone Description: Backbone Size:6682; Vector Backbone:p405; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25232010
Proper citation: RRID:Addgene_60160 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RdLuc
Vector Backbone Description: Backbone Marker:DNA 2.0; Backbone Size:2707; Vector Backbone:pJ204; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25232010
Proper citation: RRID:Addgene_60117 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: promoter
Vector Backbone Description: Backbone Size:6000; Vector Backbone:p416; Vector Types:Yeast Expression, Synthetic Biology, Yeast synthetic hybrid promoter construct; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22565375
Proper citation: RRID:Addgene_60326 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TUB2
Vector Backbone Description: Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24633327
Comments: C-terminal tail is from TUBB1 (tubulin, beta 1 class VI) according to HUGO nomenclature: http://www.genenames.org/genefamilies/TUB
Proper citation: RRID:Addgene_60400 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TUB2
Vector Backbone Description: Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24633327
Proper citation: RRID:Addgene_60405 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TUB2
Vector Backbone Description: Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24633327
Proper citation: RRID:Addgene_60404 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TUB2
Vector Backbone Description: Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24633327
Proper citation: RRID:Addgene_60402 Copy
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