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Species: Homo sapiens
Genetic Insert: Epac1 CAAX
Vector Backbone Description: Backbone Marker:invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16507992
Proper citation: RRID:Addgene_118312 Copy
Species: Homo sapiens
Genetic Insert: RalGDS
Vector Backbone Description: Backbone Marker:invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20547757
Proper citation: RRID:Addgene_118315 Copy
Species: Homo sapiens
Genetic Insert: PKI nls (aa 2-36)
Vector Backbone Description: Backbone Marker:clontech; Vector Backbone:peGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11390399
Comments: PKI constructs were subcloned into pEGFP containing Flag epitope with EcoRI and XbaI (see map).
Proper citation: RRID:Addgene_118301 Copy
Species: Homo sapiens
Genetic Insert: Caspase 9 catalytic mutant
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET-23 b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10085063
Proper citation: RRID:Addgene_11830 Copy
Species: Homo sapiens
Genetic Insert: CENP-A
Vector Backbone Description: Backbone Marker:MBL, Nagoya, Aichi, Japan; Backbone Size:4656; Vector Backbone:pmKO1-MC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18778791
Comments: Bgl II-KpnI fragment of pEGFP-AF8 (Addgene plasmid # 117803) was recloned into BamHI and KpnI site of pmKO1-MC1.
Note that the cloning strategy adds additional amino acids onto the C-terminus of the insert (STVPELQQRIRETREQSLNKRPRL), but this does not interfere with plasmid function. The extra sequence includes an MCS for adding tags to the C-terminus of the insert.
Proper citation: RRID:Addgene_118224 Copy
Species: Homo sapiens
Genetic Insert: Caspase 3
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET-23 b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9065443
Proper citation: RRID:Addgene_11821 Copy
Species: Homo sapiens
Genetic Insert: Caspase 9
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET-23 b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10085063
Proper citation: RRID:Addgene_11829 Copy
Species: Homo sapiens
Genetic Insert: Caspase 8 minus Death Effecter Domain catalytic mutant
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET-15 b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9065443
Proper citation: RRID:Addgene_11828 Copy
Species: Homo sapiens
Genetic Insert: Caspase 6
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET-23 b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9065443
Proper citation: RRID:Addgene_11823 Copy
Species: Homo sapiens
Genetic Insert: Caspase 7
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET-23 b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9065443
Proper citation: RRID:Addgene_11825 Copy
Species: Homo sapiens
Genetic Insert: Cavin3 (altered codon usage)
Vector Backbone Description: Backbone Marker:RZPD; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29621251
Proper citation: RRID:Addgene_108890 Copy
Species: Homo sapiens
Genetic Insert: Cavin1 (altered codon usage)
Vector Backbone Description: Backbone Marker:RZPD; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29621251
Proper citation: RRID:Addgene_108888 Copy
Species: Homo sapiens
Genetic Insert: H2B (human amino acid sequence but different codon usage)
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR 221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29621251
Proper citation: RRID:Addgene_108883 Copy
Species: Homo sapiens
Genetic Insert: Cavin3 (altered codon usage)
Vector Backbone Description: Backbone Marker:RZPD; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29621251
Proper citation: RRID:Addgene_108889 Copy
Species: Homo sapiens
Genetic Insert: PP2A Aalpha
Vector Backbone Description: Backbone Marker:Hahn lab, Addgene plasmid 9044; Backbone Size:6500; Vector Backbone:pMIG; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16166293
Proper citation: RRID:Addgene_10884 Copy
Species: Homo sapiens
Genetic Insert: Tau
Vector Backbone Description: Backbone Marker:EMB Biosciences; Backbone Size:6742; Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_108867 Copy
Species: Homo sapiens
Genetic Insert: EPHA2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26363067
Proper citation: RRID:Addgene_108852 Copy
Species: Homo sapiens
Genetic Insert: MYC
Vector Backbone Description: Backbone Size:6470; Vector Backbone:pMIR-REPORT Luciferase; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29249359
Proper citation: RRID:Addgene_108924 Copy
Species: Homo sapiens
Genetic Insert: FTO
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-basic; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29249359
Proper citation: RRID:Addgene_108923 Copy
Species: Homo sapiens
Genetic Insert: ATL2-deltaTM
Vector Backbone Description: Backbone Marker:Michael Rape; Backbone Size:9005; Vector Backbone:pLenti-X1-Neo; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30143524
Comments: This ATL2 construct has suspected genomic instability and/or leaky expression that results in bacteria toxicity if grown at 37'C. We recommend bacteria culture at 30'C for 24-36hrs and in large batch for significant yield.
Proper citation: RRID:Addgene_109021 Copy
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