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Species: Homo sapiens
Genetic Insert: STK16
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:p11; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: http://www.thesgc.org/structures/2BUJ/
Proper citation: RRID:Addgene_39133 Copy
Species: Homo sapiens
Genetic Insert: RGS16
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pLIC-SGC1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: http://www.thesgc.org/structures/2BT2/
Proper citation: RRID:Addgene_39140 Copy
Species: Homo sapiens
Genetic Insert: eGFP-TALEN-04-Left
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32285); Backbone Size:6447; Vector Backbone:JDS70; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TGGAGTACAA
To reduce the chance of recombination in this plasmid, do not grow in liquid culture for more than 12-14 hours.
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 1.5kb and 5.8kb
Proper citation: RRID:Addgene_39364 Copy
Species: Homo sapiens
Genetic Insert: eGFP-TALEN-01-Left
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32290); Backbone Size:6447; Vector Backbone:JDS78; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TCGCCACCAT
To reduce the chance of recombination in this plasmid, do not grow in liquid culture for more than 12-14 hours.
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 1.5kb and 5.8kb
Proper citation: RRID:Addgene_39358 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: urg1 promoter
Vector Backbone Description: Backbone Marker:Addgene plasmid # 39280; Backbone Size:2300; Vector Backbone:pFA6a-kanMX6-P3nmt1; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18197241
Proper citation: RRID:Addgene_39351 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: urg1 promoter
Vector Backbone Description: Backbone Marker:Addgene plasmid # 39280; Backbone Size:2300; Vector Backbone:pFA6a-kanMX6-P3nmt1; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18197241
Proper citation: RRID:Addgene_39353 Copy
Species: Homo sapiens
Genetic Insert: eGFP-TALEN-02-Left
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32290); Backbone Size:6447; Vector Backbone:JDS78; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TGGTGCCCAT
To reduce the chance of recombination in this plasmid, do not grow in liquid culture for more than 12-14 hours.
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 1.5kb and 5.8kb
Proper citation: RRID:Addgene_39360 Copy
Species: Homo sapiens
Genetic Insert: scFv-Fc-fusion specific for Notch2 (clone B9)
Vector Backbone Description: Backbone Marker:McCafferty lab unpublished; Vector Backbone:pBIOCAM5-3F; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22842086
Comments: Binds to both mouse mNRR2 and human hNRR2. but neither NRR1.
Proper citation: RRID:Addgene_39348 Copy
Species: Homo sapiens
Genetic Insert: ERK1
Vector Backbone Description: Vector Backbone:NpT7-5; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8444886
Proper citation: RRID:Addgene_39229 Copy
Species: Homo sapiens
Genetic Insert: HERC2 HECT Domain
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21193487
Comments: HECT domain active site point mutation (C4762A substitution)
Proper citation: RRID:Addgene_39228 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: urg1 promoter
Vector Backbone Description: Backbone Marker:Addgene plasmid # 39280; Backbone Size:2300; Vector Backbone:pFA6a-kanMX6-P3nmt1; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18197241
Proper citation: RRID:Addgene_39349 Copy
Species: Homo sapiens
Genetic Insert: scFv-Fc-fusion specific for Notch1 (clone B9)
Vector Backbone Description: Backbone Marker:McCafferty lab unpublished; Vector Backbone:pBIOCAM5-3F; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22842086
Comments: Binds to both mouse mNRR1 and human hNRR1 but not NRR2.
Proper citation: RRID:Addgene_39344 Copy
Species: Homo sapiens
Genetic Insert: scFv-Fc-fusion specific for Desmin (clone D7)
Vector Backbone Description: Backbone Marker:McCafferty lab unpublished; Vector Backbone:pBIOCAM5-3F; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18047641
Proper citation: RRID:Addgene_39346 Copy
Species: Homo sapiens
Genetic Insert: scFv specific for Jagged (clone D5)
Vector Backbone Description: Vector Backbone:pBIOCAM5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18047641
Proper citation: RRID:Addgene_39345 Copy
Genetic Insert: KanR
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717240
Proper citation: RRID:Addgene_39296 Copy
Genetic Insert: KanR
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717240
Comments: 1nt mismatch at bp#102 which may affect NheI site. Should not effect plasmid function.
Proper citation: RRID:Addgene_39295 Copy
Species: Homo sapiens
Genetic Insert: EB1
Vector Backbone Description: Backbone Size:2961; Vector Backbone:pBluescript II; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Do not cut out EB1 using EcoR1 as there is an internal EcoR1 site in the EB1 sequence.
Proper citation: RRID:Addgene_39324 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: nmt1 promoter
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717240
Proper citation: RRID:Addgene_39285 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: nmt1 promoter
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717240
Proper citation: RRID:Addgene_39284 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: nmt1 promoter
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717240
Comments: Use GST reverse primer to verify mutation in nmt1 promoter
Proper citation: RRID:Addgene_39286 Copy
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