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Species: Synthetic
Genetic Insert: dCas9-hudTET1CD, SgRNA6 (huTSDR)
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 48138); Vector Backbone:PX458 ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33488615
Proper citation: RRID:Addgene_129046 Copy
Species: Synthetic
Genetic Insert: dCas9-huTET1CD, SgRNA cloning site
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 48138); Vector Backbone:PX458 ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33488615
Comments: For SgRNA cloning: Caution! BbsI cannot be used for SgRNA cloning in this plasmid, there are BbsI sites in TET1CD. Instead the guide sequence can be cloned into Addgene#48138 and in a second step transferred into this plasmid (for example using PvuI & XbaI).
This plasmid was generated by Christopher Kressler.
Proper citation: RRID:Addgene_129027 Copy
Species: Synthetic
Genetic Insert: dCas9-huTET1CD, SgRNA cloning site
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 48138); Vector Backbone:PX458 ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33488615
Comments: For SgRNA cloning: Caution! BbsI cannot be used for SgRNA cloning in this plasmid, there are BbsI sites in TET1CD. Instead the guide sequence can be cloned into Addgene#48138 and in a second step transferred into this plasmid (for example using PvuI & XbaI).
This Plasmid was generated by Christopher Kressler.
Proper citation: RRID:Addgene_129025 Copy
Species: Branchiostoma lanceolatum
Genetic Insert: mNeonGreen
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: To design forward PCR primer for C-terminal tagging, use: 5'-(gene-specific sequence) ATGGTGAGTAAAGGCGAGGA-3'. To design reverse PCR primer for C-terminal tagging, use: 5'-(gene-specific sequence) GAATTCGAGCTCGTTTAAAC-3'.
Proper citation: RRID:Addgene_129099 Copy
Species: Homo sapiens
Genetic Insert: cJun
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5370; Vector Backbone:pET 29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31857650
Comments: Depositor uses BL21 for protein production.
Proper citation: RRID:Addgene_129227 Copy
Species: Homo sapiens
Genetic Insert: Microtubule-associated proteins 1A/1B light chain 3B
Vector Backbone Description: Backbone Size:2793; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31315929
Proper citation: RRID:Addgene_129291 Copy
Species: Synthetic
Genetic Insert: QUEEN-37C
Vector Backbone Description: Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Depositor Adm #1042 . Please visit https://www.biorxiv.org/content/10.1101/2021.10.08.463131v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_129318 Copy
Species: Homo sapiens
Genetic Insert: MED1-IDR long
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32386547
Proper citation: RRID:Addgene_145276 Copy
Vector Backbone Description: Vector Backbone:pmCellFree_KA2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:32081002
Proper citation: RRID:Addgene_145369 Copy
Species: Homo sapiens
Genetic Insert: sodium voltage-gated channel alpha subunit 5
Vector Backbone Description: Backbone Size:5015; Vector Backbone:pCDNA3.1-Zeo (+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15548568
Proper citation: RRID:Addgene_145374 Copy
Species: Homo sapiens
Genetic Insert: PPP2R5C B56 epsilon regulatory subunit of PP2A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10186; Vector Backbone:pCEP4 (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10092233
Comments: 4 HA tag cloned into Not1 site as Not1 cassette so two Not1 sites at 5' end of gene.
Proper citation: RRID:Addgene_14537 Copy
Species: Mus musculus
Genetic Insert: MKK7a1
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9207092
Proper citation: RRID:Addgene_14538 Copy
Species: Homo sapiens
Genetic Insert: PPP2R5D B56 delta regulatory subunit of PP2A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10186; Vector Backbone:pCEP4 (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10092233
Comments: 4 HA tag cloned into Not1 site as Not1 cassette so two Not1 sites at 5' end of gene. Please note that the Addgene verified sequence finds an R210H mutation in PPP2R5D. Other differences compared to the depositor sequence do not affect plasmid function.
Proper citation: RRID:Addgene_14536 Copy
Species: Homo sapiens
Genetic Insert: PPP2R5B B56 beta regulatory subunit of PP2A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10186; Vector Backbone:pCEP4 (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10092233
Comments: 4 HA tag cloned into Not1 site as Not1 cassette so two Not1 sites at 5' end of gene. Addgene NGS identified an I346V mutation in the B56β ORF, this is not predicted to impact plasmid function.
Proper citation: RRID:Addgene_14533 Copy
Species: Homo sapiens
Genetic Insert: PPP2R5A B56 alpha regulatory subunit of PP2A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10186; Vector Backbone:pCEP4 (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10092233
Comments: 4 HA tag cloned into Not1 site as Not1 cassette so two Not1 sites at 5' end of gene.
Proper citation: RRID:Addgene_14532 Copy
Species: Mus musculus
Genetic Insert: MKK7a1(Ala)
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9207092
Proper citation: RRID:Addgene_14539 Copy
Species: Homo sapiens
Genetic Insert: ATM shRNA
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRDI292; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15709017
Comments: Sense oligo sequence used for cloning: ATM, 5'-GATCCCCGGA TTTGCGTATT ACTCAGTTCA AGAGACTGAG TAATACGCAA ATCCTTTTTG GAAA-3'.
Proper citation: RRID:Addgene_14542 Copy
Species: Homo sapiens
Genetic Insert: ATR shRNA
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRDI292; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15709017
Comments: Sense oligo sequence used for cloning: ATR, 5'-GATCCCCGGC GTCGTCTCAG CTCGTCTTCA AGAGAGACGA GCTGAGACGA CGCCTTTTTG GAAA-3'.
Proper citation: RRID:Addgene_14543 Copy
Species: Mus musculus
Genetic Insert: MKK7a1(Glu)
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9207092
Proper citation: RRID:Addgene_14540 Copy
Species: Homo sapiens
Genetic Insert: Vav1
Vector Backbone Description: Backbone Size:0; Vector Backbone:pMX-uGFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16709244
Comments: The Gateway cloning system (Invitrogen) was used to subclone human Vav1 into pMX-uGFP.
Proper citation: RRID:Addgene_14557 Copy
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