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pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-6
 
Resource Report
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1+ mentions 		RRID:Addgene_129046 dCas9-hudTET1CD, SgRNA6 (huTSDR) Synthetic Ampicillin PMID:33488615 Backbone Marker:Feng Zhang (Addgene plasmid # 48138); Vector Backbone:PX458 ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin dCas9 (D10A;H840A), catalytic domain huTET1 inactive (H1671Y; D1673A) 2026-08-15 01:04:54 1
pSpdCas9-huTET1CD-T2A-mCherry(PX458)
 
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1+ mentions 		RRID:Addgene_129027 dCas9-huTET1CD, SgRNA cloning site Synthetic Ampicillin PMID:33488615 For SgRNA cloning: Caution! BbsI cannot be used for SgRNA cloning in this plasmid, there are BbsI sites in TET1CD. Instead the guide sequence can be cloned into Addgene#48138 and in a second step transferred into this plasmid (for example using PvuI & XbaI). This plasmid was generated by Christopher Kressler. Backbone Marker:Feng Zhang (Addgene plasmid # 48138); Vector Backbone:PX458 ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin dCas9 (D10A;H840A) 2026-08-15 01:04:54 1
pSpdCas9-huTET1CD-T2A-GFP (PX458)
 
Resource Report
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1+ mentions 		RRID:Addgene_129025 dCas9-huTET1CD, SgRNA cloning site Synthetic Ampicillin PMID:33488615 For SgRNA cloning: Caution! BbsI cannot be used for SgRNA cloning in this plasmid, there are BbsI sites in TET1CD. Instead the guide sequence can be cloned into Addgene#48138 and in a second step transferred into this plasmid (for example using PvuI & XbaI). This Plasmid was generated by Christopher Kressler. Backbone Marker:Feng Zhang (Addgene plasmid # 48138); Vector Backbone:PX458 ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin dCas9 (D10A;H840A) 2026-08-15 01:04:54 1
pFA6a-mNeonGreen-KanMX6
 
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1+ mentions 		RRID:Addgene_129099 mNeonGreen Branchiostoma lanceolatum Ampicillin To design forward PCR primer for C-terminal tagging, use: 5'-(gene-specific sequence) ATGGTGAGTAAAGGCGAGGA-3'. To design reverse PCR primer for C-terminal tagging, use: 5'-(gene-specific sequence) GAATTCGAGCTCGTTTAAAC-3'. Vector Backbone:pFA6a; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:04:55 2
pET-29b-3xGluGlu-c-jun-His6
 
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1+ mentions 		RRID:Addgene_129227 cJun Homo sapiens Kanamycin PMID:31857650 Depositor uses BL21 for protein production. Backbone Marker:EMD Biosciences; Backbone Size:5370; Vector Backbone:pET 29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:04:55 1
pDONR223 LC3B Q116P G120
 
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1+ mentions 		RRID:Addgene_129291 Microtubule-associated proteins 1A/1B light chain 3B Homo sapiens Spectinomycin PMID:31315929 Backbone Size:2793; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin Changed Glutamine 116 to Proline. Deleted amino acids 121-125 (stop codon after G120). 2026-08-15 01:04:55 1
pN1-QUE37C
 
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1+ mentions 		RRID:Addgene_129318 QUEEN-37C Synthetic Kanamycin Depositor Adm #1042 . Please visit https://www.biorxiv.org/content/10.1101/2021.10.08.463131v1 for bioRxiv preprint. Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:04:56 1
pHR-MCS-MED1long-IDR-YFP-NLS
 
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1+ mentions 		RRID:Addgene_145276 MED1-IDR long Homo sapiens Ampicillin PMID:32386547 Vector Backbone:pHR; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:06:54 1
pmCellFree_KA1
 
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1+ mentions 		RRID:Addgene_145369 Chloramphenicol and Ampicillin PMID:32081002 Vector Backbone:pmCellFree_KA2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:06:55 1
pCDNA3.1 hSCN5A
 
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1+ mentions 		RRID:Addgene_145374 sodium voltage-gated channel alpha subunit 5 Homo sapiens Ampicillin PMID:15548568 Backbone Size:5015; Vector Backbone:pCDNA3.1-Zeo (+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:06:55 2
V250 pCEP-4HA B56epsilon
 
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1+ mentions 		RRID:Addgene_14537 PPP2R5C B56 epsilon regulatory subunit of PP2A Homo sapiens Ampicillin PMID:10092233 4 HA tag cloned into Not1 site as Not1 cassette so two Not1 sites at 5' end of gene. Backbone Marker:Invitrogen; Backbone Size:10186; Vector Backbone:pCEP4 (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:06:55 6
pCDNA3 Flag MKK7a1
 
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1+ mentions 		RRID:Addgene_14538 MKK7a1 Mus musculus Ampicillin PMID:9207092 Backbone Marker:invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:06:55 3
V249 pCEP-4HA B56delta
 
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1+ mentions 		RRID:Addgene_14536 PPP2R5D B56 delta regulatory subunit of PP2A Homo sapiens Ampicillin PMID:10092233 4 HA tag cloned into Not1 site as Not1 cassette so two Not1 sites at 5' end of gene. Please note that the Addgene verified sequence finds an R210H mutation in PPP2R5D. Other differences compared to the depositor sequence do not affect plasmid function. Backbone Marker:Invitrogen; Backbone Size:10186; Vector Backbone:pCEP4 (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin R210H 2026-08-15 01:06:55 8
V246 pCEP 4HA B56beta
 
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1+ mentions 		RRID:Addgene_14533 PPP2R5B B56 beta regulatory subunit of PP2A Homo sapiens Ampicillin PMID:10092233 4 HA tag cloned into Not1 site as Not1 cassette so two Not1 sites at 5' end of gene. Addgene NGS identified an I346V mutation in the B56β ORF, this is not predicted to impact plasmid function. Backbone Marker:Invitrogen; Backbone Size:10186; Vector Backbone:pCEP4 (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin I346V relative to GenBank ID L42374 2026-08-15 01:06:54 5
V245 pCEP-4HA B56alpha
 
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10+ mentions 		RRID:Addgene_14532 PPP2R5A B56 alpha regulatory subunit of PP2A Homo sapiens Ampicillin PMID:10092233 4 HA tag cloned into Not1 site as Not1 cassette so two Not1 sites at 5' end of gene. Backbone Marker:Invitrogen; Backbone Size:10186; Vector Backbone:pCEP4 (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:06:54 13
pCDNA3 Flag MKK7a1 (ala)
 
Resource Report
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1+ mentions 		RRID:Addgene_14539 MKK7a1(Ala) Mus musculus Ampicillin PMID:9207092 Backbone Marker:invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Ser-198Ala Thr-202Ala 2026-08-15 01:06:55 3
pLV.ATMi
 
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1+ mentions 		RRID:Addgene_14542 ATM shRNA Homo sapiens Ampicillin PMID:15709017 Sense oligo sequence used for cloning: ATM, 5'-GATCCCCGGA TTTGCGTATT ACTCAGTTCA AGAGACTGAG TAATACGCAA ATCCTTTTTG GAAA-3'. Backbone Size:0; Vector Backbone:pRDI292; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin 2026-08-15 01:06:56 5
pLV.ATRi
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_14543 ATR shRNA Homo sapiens Ampicillin PMID:15709017 Sense oligo sequence used for cloning: ATR, 5'-GATCCCCGGC GTCGTCTCAG CTCGTCTTCA AGAGAGACGA GCTGAGACGA CGCCTTTTTG GAAA-3'. Backbone Size:0; Vector Backbone:pRDI292; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin 2026-08-15 01:06:56 1
pCDNA3 Flag MKK7a1 (glu)
 
Resource Report
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1+ mentions 		RRID:Addgene_14540 MKK7a1(Glu) Mus musculus Ampicillin PMID:9207092 Backbone Marker:invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Ser-198Glu Thr-202Glu 2026-08-15 01:06:55 3
pMX Vav1 uGFP
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_14557 Vav1 Homo sapiens Ampicillin PMID:16709244 The Gateway cloning system (Invitrogen) was used to subclone human Vav1 into pMX-uGFP. Backbone Size:0; Vector Backbone:pMX-uGFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-15 01:06:57 4

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