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Species: Other
Genetic Insert: promotor Ubi4-2 (1000bp)
Vector Backbone Description: Vector Backbone:pAGM1251 (Engler et al., 2014); Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29847550
Proper citation: RRID:Addgene_105383 Copy
Species: Other
Genetic Insert: promotor Zea mays Ubiquitin (1500bp)
Vector Backbone Description: Vector Backbone:pICH41295 (Engler et al., 2014); Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29847550
Proper citation: RRID:Addgene_105384 Copy
Species: Other
Genetic Insert: mYFP (GFP variant) fused to SV40 nuclear localization signal
Vector Backbone Description: Vector Backbone:pAGM1301 (Engler et al., 2014); Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29847550
Proper citation: RRID:Addgene_105398 Copy
Species: Other
Genetic Insert: PKI nuclear export signal
Vector Backbone Description: Vector Backbone:pAGM1276 (Engler et al., 2014); Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29847550
Proper citation: RRID:Addgene_105399 Copy
Species: Other
Genetic Insert: mYFP (GFP variant) fused to PKI nuclear export signal
Vector Backbone Description: Vector Backbone:pAGM1301 (Engler et al., 2014); Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29847550
Proper citation: RRID:Addgene_105397 Copy
Species: Other
Genetic Insert: mTRQ (GFP variant)
Vector Backbone Description: Vector Backbone:pAGM1301 (Engler et al., 2014); Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29847550
Proper citation: RRID:Addgene_105394 Copy
Species: Other
Genetic Insert: mVenus (GFP variant)
Vector Backbone Description: Vector Backbone:pAGM1276 (Engler et al., 2014); Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29847550
Proper citation: RRID:Addgene_105395 Copy
Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:modified pEGFP-C1; Vector Backbone:pIL2RNB; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20656375
Comments: pIL2R was constructed by replacing EGFP with transmembrane domain of IL2R using Nhe1 and BglII sites. The mCherry sequence was inserted into HindIII and Xba1 sites of pIL2R NBC1. mCherry was from Dr. Roger Tsien (UCSD).
Proper citation: RRID:Addgene_105317 Copy
Species: Other
Genetic Insert: Gln-tRNA synthetase
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29131146
Comments: Gene comes from E.coli.
Proper citation: RRID:Addgene_111490 Copy
Species: Other
Genetic Insert: Tyr-tRNA synthetase
Vector Backbone Description: Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29131146
Comments: Gene comes from E.coli.
Proper citation: RRID:Addgene_111491 Copy
Species: Other
Genetic Insert: translational initiation factor 1
Vector Backbone Description: Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29131146
Comments: Gene comes from E.coli.
Proper citation: RRID:Addgene_111493 Copy
Species: Other
Genetic Insert: yhjH
Vector Backbone Description: Vector Backbone:pJN105 (pBBR1-based); Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:23690403
Comments: A52V mutation in yhjH does not alter enzyme function. The expressed YhjH enzyme is a functional phosphodiesterase that degrades cyclic di-GMP.
Proper citation: RRID:Addgene_111613 Copy
Species: Other
Genetic Insert: APEX2
Vector Backbone Description: Vector Backbone:pMV306; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29708735
Comments: This plasmid is for theophylline-inducible expression of APEX2 in the periplasm of mycobacteria. It is a single-copy integrating vector in mycobacteria. The APEX gene was subcloned from pcDNA3-mito-APEX and subsequently mutated by site-directed mutagenesis to generate the optimized variant APEX2.
See also references: Rhee HW, Zou P, Udeshi ND, Martell JD, Mootha VK, Carr SA, Ting AY. Proteomic mapping of mitochondria in living cells via spatially restricted enzymatic tagging. Science. 2013;339(6125):1328-31. PMCID: 3916822.
Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, Ting AY. Directed evolution of APEX2 for electron microscopy and proximity labeling. Nat Methods. 2015;12(1):51-4. PMCID: 4296904
Proper citation: RRID:Addgene_111701 Copy
Species: Other
Genetic Insert: LacI_I12X86
Vector Backbone Description: Vector Backbone:pAA.3; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29724956
Proper citation: RRID:Addgene_112025 Copy
Species: Other
Genetic Insert: TaU6-LacZ-sgRNA
Vector Backbone Description: Backbone Marker:Unknown; Backbone Size:4000; Vector Backbone:Unknown; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30460568
Proper citation: RRID:Addgene_112028 Copy
Species: Other
Genetic Insert: AtCAS9 nickase D10A
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Comments: BsaI and BbsI sites have been removed from the sequence by site-directed mutagenesis, however the amino acid sequence is as in the original AtCas9 pDe-CAS9-D10A vector from H. Puchta lab.
Proper citation: RRID:Addgene_112071 Copy
Species: Other
Genetic Insert: AtDeadCAS9 D10A H840A
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Comments: BsaI and BbsI sites have been removed from the sequence by site-directed mutagenesis.
Proper citation: RRID:Addgene_112072 Copy
Species: Other
Genetic Insert: TagRFP
Vector Backbone Description: Vector Backbone:pICH41258; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Comments: BsaI and BbsI sites have been removed from the sequence by site-directed mutagenesis.
Proper citation: RRID:Addgene_112077 Copy
Species: Other
Genetic Insert: pcUBI promoter
Vector Backbone Description: Vector Backbone:pICH41295; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Comments: BsaI and BbsI sites have been removed from the sequence by site-directed mutagenesis.
Proper citation: RRID:Addgene_112075 Copy
Species: Other
Genetic Insert: AtCAS9 D10A without stop codon
Vector Backbone Description: Vector Backbone:pAGM1287; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Comments: BsaI and BbsI sites have been removed from the sequence by site-directed mutagenesis.
Proper citation: RRID:Addgene_112076 Copy
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