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Species: Homo sapiens
Genetic Insert: Vav1
Vector Backbone Description: Backbone Size:0; Vector Backbone:pMX-uGFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16709244
Comments: The Gateway cloning system (Invitrogen) was used to subclone human Vav1 into pMX-uGFP.
Proper citation: RRID:Addgene_14558 Copy
Species: Homo sapiens
Genetic Insert: Vav2
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pC.HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10938113
Proper citation: RRID:Addgene_14554 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 nsp4
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725300.1Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145495 Copy
Species: Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp8
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009725304.1
Proper citation: RRID:Addgene_145584 Copy
Species: Homo sapiens
Genetic Insert: cdc42 Q61L
Vector Backbone Description: Backbone Size:0; Vector Backbone:pMX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16546099
Proper citation: RRID:Addgene_14568 Copy
Species: Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 S (surface glycoprotein)
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009724390.1
Proper citation: RRID:Addgene_145730 Copy
Species: Bos taurus
Genetic Insert: PIK3CA E545K
Vector Backbone Description: Backbone Marker:Jeff Engelman; Backbone Size:0; Vector Backbone:JP1520; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16322248
Comments: Hemagglutinin (HA)–tagged bovine PIK3CA cDNA was subcloned into the pDNR-Dual vector (Clontech, Mountain View, CA), and site-directed mutagenesis (Quick Change, Stratagene, La Jolla, CA) was used to generate the E545K mutation. The cDNA was shuttled into the JP1520 retroviral vector using the BD Creator System (Clontech).
Proper citation: RRID:Addgene_14571 Copy
Genetic Insert: PIK3CA
Vector Backbone Description: Backbone Marker:Jeff Engelman; Backbone Size:0; Vector Backbone:JP1520; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16322248
Comments: Hemagglutinin (HA)–tagged bovine PIK3CA cDNA was subcloned into the pDNR-Dual vector (Clontech, Mountain View, CA). The cDNA was shuttled into the JP1520 retroviral vector using the BD Creator System (Clontech).
Proper citation: RRID:Addgene_14570 Copy
Species: Homo sapiens
Genetic Insert: kappa-myc-dK-2xG4S-TMst
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5358; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32786268
Comments: Please visit the Molecular Biosensor and Imaging Center website for current protocols and information about dyes to be used in combination with the proteins expressed from this plasmid.
http://pathways.mbic.cmu.edu/
For more information about this plasmid, please see the associated publication (PMID: 32786268).
Proper citation: RRID:Addgene_145766 Copy
Species: HIV-1
Genetic Insert: Capsid
Vector Backbone Description: Backbone Marker:Bio-Rad; Backbone Size:2863; Vector Backbone:pTZ19U; Vector Types:Mammalian Expression, Lentiviral, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33033190
Proper citation: RRID:Addgene_145784 Copy
Species: Oryza Sativa
Genetic Insert: Pong TPase L418A, L420A
Vector Backbone Description: Vector Backbone:pAG415 GAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20226077
Proper citation: RRID:Addgene_145795 Copy
Species: Homo sapiens
Genetic Insert: ATR shRNA
Vector Backbone Description: Backbone Size:3176; Vector Backbone:pSUPER; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15709017
Comments: Sense oligo sequence used for cloning: ATR, 5'-GATCCCCGGC GTCGTCTCAG CTCGTCTTCA AGAGAGACGA GCTGAGACGA CGCCTTTTTG GAAA-3'.
Proper citation: RRID:Addgene_14582 Copy
Species: Homo sapiens
Genetic Insert: ATM shRNA
Vector Backbone Description: Backbone Size:3176; Vector Backbone:pSUPER; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15709017
Comments: Sense oligo sequence used for cloning: ATM, 5'-GATCCCCGGA TTTGCGTATT ACTCAGTTCA AGAGACTGAG TAATACGCAA ATCCTTTTTG GAAA-3'.
Proper citation: RRID:Addgene_14581 Copy
Species: Homo sapiens
Genetic Insert: human TMPRSS2 variant 1
Vector Backbone Description: Vector Backbone:RRL.sin.cPPT.SFFV/IRES-neo.WPRE. ; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33514628
Comments: NM_001135099 with 1 mutation G8V
Proper citation: RRID:Addgene_145843 Copy
Vector Backbone Description: Vector Backbone:pT2AL200R175-CAGGS-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32456235
Proper citation: RRID:Addgene_145813 Copy
Species: E. coli
Genetic Insert: LexA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5600; Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15203006
Proper citation: RRID:Addgene_14594 Copy
Species: Homo sapiens
Genetic Insert: cyclin T1
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX 2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9990016
Comments: There is a H519R mutation in the ORF.
Proper citation: RRID:Addgene_14607 Copy
Species: Mus musculus
Genetic Insert: MKK7b1
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9891090
Proper citation: RRID:Addgene_14622 Copy
Species: Homo sapiens
Genetic Insert: CD4
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pMX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3260352
Proper citation: RRID:Addgene_14614 Copy
Species: Homo sapiens
Genetic Insert: MKK4
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7839144
Comments: ORF starts at aa15. Depositing lab says this does not affect plasmid function.
Proper citation: RRID:Addgene_14615 Copy
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