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Species: Homo sapiens
Genetic Insert: ANGPTL3
Vector Backbone Description: Backbone Size:4989; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29031715
Comments: 21bp deletion in the SV40 promoter sequence. May affect blasticidin selection.
Proper citation: RRID:Addgene_109112 Copy
Species: Homo sapiens
Genetic Insert: ANGPTL8
Vector Backbone Description: Backbone Size:5058; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29031715
Proper citation: RRID:Addgene_109114 Copy
Species: Homo sapiens
Genetic Insert: ANGPTL8
Vector Backbone Description: Backbone Size:8031; Vector Backbone:pRRL-cPPT-MCS-IRES-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29031715
Proper citation: RRID:Addgene_109116 Copy
Species: Homo sapiens
Genetic Insert: CTNNB1 Homology Arms with mEGFP-linker
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the N-terminus of human CTNNB1 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: SGLRSRAQASNSAVDGTAAT. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .
Proper citation: RRID:Addgene_109119 Copy
Species: Homo sapiens
Genetic Insert: human APOBEC3A
Vector Backbone Description: Vector Backbone:pET41; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30295673
Proper citation: RRID:Addgene_109231 Copy
Species: Homo sapiens
Genetic Insert: human full-length REST/NRSF
Vector Backbone Description: Backbone Marker:Geneva Biotech; Vector Backbone:pIDS; Vector Types:Insect Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28218430
Proper citation: RRID:Addgene_109162 Copy
Species: Homo sapiens
Genetic Insert: PTEN
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_10927 Copy
Species: Homo sapiens
Genetic Insert: human C-terminal CoREST, sequence-optimized for insect cells
Vector Backbone Description: Backbone Marker:Geneva Biotech; Vector Backbone:pACEBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:28218430
Proper citation: RRID:Addgene_109159 Copy
Species: Homo sapiens
Genetic Insert: PTEN
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_10926 Copy
Species: Homo sapiens
Genetic Insert: DGCR8
Vector Backbone Description: Backbone Size:5283; Vector Backbone:N/A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15589161
Proper citation: RRID:Addgene_10921 Copy
Species: Homo sapiens
Genetic Insert: mCherry-KASH
Vector Backbone Description: Vector Backbone:Custom; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30271673
Proper citation: RRID:Addgene_109321 Copy
Species: Homo sapiens
Genetic Insert: p16 INK4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7603984
Comments: A human pl6ink4 cDNA was obtained by PCR amplification of a HeLa cell cDNA library with a 5' primer (5'-GGAATTCACCACCATGGAGCCTTCGGCTGAC-3') and a 3' primer (5'-GGAATTCTCGAGTCAATCGGGGATATCTGAGGGACC-3'). A 472-bp fragment was amplified, purified on a low-melting agarose gel, and cloned directly into pGEM-T (Promega). The resulting plasmid was then used to isolate an EcoRI/Xho I fragment containing the pl6ink4 coding region, which was cloned into pcDNA3 (Invitrogen) to construct pCMV.pl6ink4.
The insert corresponds to isoform 1 but is missing the first 8 amino acids.
Proper citation: RRID:Addgene_10916 Copy
Species: Homo sapiens
Genetic Insert: Depdc5
Vector Backbone Description: Vector Backbone:pLJC5; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29590090
Proper citation: RRID:Addgene_109341 Copy
Species: Homo sapiens
Genetic Insert: Depdc5
Vector Backbone Description: Vector Backbone:pLJC5; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29590090
Proper citation: RRID:Addgene_109337 Copy
Species: Homo sapiens
Genetic Insert: GαS
Vector Backbone Description: Backbone Size:6002; Vector Backbone:pEM705; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29338718
Proper citation: RRID:Addgene_109350 Copy
Species: Homo sapiens
Genetic Insert: Gso chimera
Vector Backbone Description: Backbone Size:6002; Vector Backbone:pEM705; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29338718
Proper citation: RRID:Addgene_109353 Copy
Species: Homo sapiens
Genetic Insert: Cyclin E1
Vector Backbone Description: Vector Backbone:pInducer20; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29148972
Proper citation: RRID:Addgene_109348 Copy
Species: Homo sapiens
Genetic Insert: PTEN
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5700; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: See author's map.
Proper citation: RRID:Addgene_10936 Copy
Species: Homo sapiens
Genetic Insert: PTEN
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pFastBac 2T; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: For baculovirus expression.
Proper citation: RRID:Addgene_10937 Copy
Species: Homo sapiens
Genetic Insert: Rod opsin
Vector Backbone Description: Backbone Size:5374; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23554393
Comments: Also see Ballister et al. BMC Biol. 2018 (doi: 10.1186/s12915-017-0475-2) for additional use.
Proper citation: RRID:Addgene_109361 Copy
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