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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pHS15 (hANGPTL3-Strep) Resource Report Resource Website |
RRID:Addgene_109112 | ANGPTL3 | Homo sapiens | Ampicillin | PMID:29031715 | 21bp deletion in the SV40 promoter sequence. May affect blasticidin selection. | Backbone Size:4989; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:01:26 | 0 | |
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pEB30 (hANGPTL8-V5-His) Resource Report Resource Website |
RRID:Addgene_109114 | ANGPTL8 | Homo sapiens | Ampicillin | PMID:29031715 | Backbone Size:5058; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:01:26 | 0 | ||
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pEB31 (hANGPTL8-V5-His lenti) Resource Report Resource Website |
RRID:Addgene_109116 | ANGPTL8 | Homo sapiens | Ampicillin | PMID:29031715 | Backbone Size:8031; Vector Backbone:pRRL-cPPT-MCS-IRES-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:01:26 | 0 | ||
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AICSDP-47: CTNNB1-mEGFP Resource Report Resource Website |
RRID:Addgene_109119 | CTNNB1 Homology Arms with mEGFP-linker | Homo sapiens | Ampicillin | This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the N-terminus of human CTNNB1 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: SGLRSRAQASNSAVDGTAAT. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ . | Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin | homology arms contain point mutations to disrupt crRNA binding sites used | 2026-08-15 01:01:26 | 0 | |
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pET41-MBP-t-A3A-t-His Resource Report Resource Website 1+ mentions |
RRID:Addgene_109231 | human APOBEC3A | Homo sapiens | Kanamycin | PMID:30295673 | Vector Backbone:pET41; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:01:27 | 4 | ||
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pIDS-RESTmut full-length Resource Report Resource Website |
RRID:Addgene_109162 | human full-length REST/NRSF | Homo sapiens | Spectinomycin | PMID:28218430 | Backbone Marker:Geneva Biotech; Vector Backbone:pIDS; Vector Types:Insect Expression; Bacterial Resistance:Spectinomycin | Cys402Ser | 2026-08-15 01:01:27 | 0 | |
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830 pcDNA3 GFP PTEN 1-336 Resource Report Resource Website |
RRID:Addgene_10927 | PTEN | Homo sapiens | Ampicillin | Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Amino acids 1-336 | 2026-08-15 01:01:27 | 0 | ||
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pACEBAC1-CoREST-C Resource Report Resource Website |
RRID:Addgene_109159 | human C-terminal CoREST, sequence-optimized for insect cells | Homo sapiens | Gentamicin | PMID:28218430 | Backbone Marker:Geneva Biotech; Vector Backbone:pACEBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin | 2026-08-15 01:01:26 | 0 | ||
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827 pcDNA3 GFP PTEN 1-274 Resource Report Resource Website |
RRID:Addgene_10926 | PTEN | Homo sapiens | Ampicillin | Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Amino acids 1-274 | 2026-08-15 01:01:27 | 0 | ||
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pFLAG/HA-DGCR8 Resource Report Resource Website 10+ mentions |
RRID:Addgene_10921 | DGCR8 | Homo sapiens | Ampicillin | PMID:15589161 | Backbone Size:5283; Vector Backbone:N/A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:01:27 | 10 | ||
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pU6-crRNA(SOD1) Resource Report Resource Website |
RRID:Addgene_109321 | mCherry-KASH | Homo sapiens | Ampicillin | PMID:30271673 | Vector Backbone:Custom; Vector Types:AAV; Bacterial Resistance:Ampicillin | 2026-08-15 01:01:28 | 0 | ||
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pCMV p16 INK4A Resource Report Resource Website 1+ mentions |
RRID:Addgene_10916 | p16 INK4 | Homo sapiens | Ampicillin | PMID:7603984 | A human pl6ink4 cDNA was obtained by PCR amplification of a HeLa cell cDNA library with a 5' primer (5'-GGAATTCACCACCATGGAGCCTTCGGCTGAC-3') and a 3' primer (5'-GGAATTCTCGAGTCAATCGGGGATATCTGAGGGACC-3'). A 472-bp fragment was amplified, purified on a low-melting agarose gel, and cloned directly into pGEM-T (Promega). The resulting plasmid was then used to isolate an EcoRI/Xho I fragment containing the pl6ink4 coding region, which was cloned into pcDNA3 (Invitrogen) to construct pCMV.pl6ink4. The insert corresponds to isoform 1 but is missing the first 8 amino acids. | Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:01:26 | 5 | |
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[pSK497] pLJC5 Flag-Depdc5(AB) Resource Report Resource Website |
RRID:Addgene_109341 | Depdc5 | Homo sapiens | Ampicillin | PMID:29590090 | Vector Backbone:pLJC5; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | DIYGD(185-189)GSGSG;EQPLH(371-375)GSGSG | 2026-08-15 01:01:28 | 0 | |
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[pSK468] pLJC5 Flag-Depdc5 Resource Report Resource Website |
RRID:Addgene_109337 | Depdc5 | Homo sapiens | Ampicillin | PMID:29590090 | Vector Backbone:pLJC5; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:01:28 | 0 | ||
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pEM705 Gs Resource Report Resource Website 1+ mentions |
RRID:Addgene_109350 | GαS | Homo sapiens | Ampicillin | PMID:29338718 | Backbone Size:6002; Vector Backbone:pEM705; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:01:28 | 1 | ||
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pEM705 Gso (Ser) Resource Report Resource Website |
RRID:Addgene_109353 | Gso chimera | Homo sapiens | Ampicillin | PMID:29338718 | Backbone Size:6002; Vector Backbone:pEM705; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Changed Cysteine at position 391 to Serine (Pertussis toxin insensitive) | 2026-08-15 01:01:28 | 0 | |
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pInducer20 Cyclin E1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_109348 | Cyclin E1 | Homo sapiens | Ampicillin | PMID:29148972 | Vector Backbone:pInducer20; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:01:28 | 2 | ||
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883 pET15b PTEN Resource Report Resource Website |
RRID:Addgene_10936 | PTEN | Homo sapiens | Ampicillin | See author's map. | Backbone Marker:EMD Biosciences; Backbone Size:5700; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:01:28 | 0 | ||
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891 pFastBac GST PTEN Resource Report Resource Website |
RRID:Addgene_10937 | PTEN | Homo sapiens | Ampicillin | For baculovirus expression. | Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pFastBac 2T; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:01:28 | 0 | ||
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pcDNA3 Rod Opsin Resource Report Resource Website 1+ mentions |
RRID:Addgene_109361 | Rod opsin | Homo sapiens | Ampicillin | PMID:23554393 | Also see Ballister et al. BMC Biol. 2018 (doi: 10.1186/s12915-017-0475-2) for additional use. | Backbone Size:5374; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:01:28 | 2 |
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