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Species: Synthetic
Genetic Insert: 6x His epitope tag + polyA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2555; Vector Backbone:pDONR221 P3-P2; Vector Types:Gateway Cloning, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30590691
Proper citation: RRID:Addgene_121754 Copy
Species: Synthetic
Genetic Insert: Gateway selection cassette (ccdb gene + Chlor resistance gene)
Vector Backbone Description: Backbone Marker:Newgard Lab; Backbone Size:4657; Vector Backbone:JN607; Vector Types:Mammalian Expression, Sleeping Beauty Transposon, pMVP Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30590691
Comments: Sleeping Beauty ITR sequences derived from Addgene #26563
Proper citation: RRID:Addgene_121866 Copy
Species: Synthetic
Genetic Insert: BirA
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30858523
Proper citation: RRID:Addgene_121907 Copy
Species: Synthetic
Genetic Insert: Gateway selection cassette (ccdb gene + Chlor resistance gene)
Vector Backbone Description: Backbone Marker:Newgard Lab; Backbone Size:4575; Vector Backbone:JN607; Vector Types:Mammalian Expression, Sleeping Beauty Transposon, pMVP Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30590691
Comments: Sleeping Beauty ITR sequences derived from Addgene #26559
Proper citation: RRID:Addgene_121862 Copy
Species: Synthetic
Genetic Insert: Gateway selection cassette (ccdb gene + Chlor resistance gene)
Vector Backbone Description: Backbone Marker:Newgard Lab; Backbone Size:4261; Vector Backbone:JN607; Vector Types:Mammalian Expression, Sleeping Beauty Transposon, pMVP Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30590691
Comments: Sleeping Beauty ITR sequences derived from Addgene #26561
Proper citation: RRID:Addgene_121864 Copy
Species: Synthetic
Genetic Insert: Gateway selection cassette (ccdb gene + Chlor resistance gene)
Vector Backbone Description: Backbone Marker:Newgard Lab; Backbone Size:4462; Vector Backbone:JN607; Vector Types:Mammalian Expression, Sleeping Beauty Transposon, pMVP Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30590691
Comments: Sleeping Beauty ITR sequences derived from Addgene #26562
Proper citation: RRID:Addgene_121865 Copy
Species: Synthetic
Genetic Insert: Gateway selection cassette (ccdb gene + Chlor resistance gene)
Vector Backbone Description: Backbone Marker:Newgard Lab; Backbone Size:3806; Vector Backbone:JK104; Vector Types:Mammalian Expression, pMVP Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30590691
Proper citation: RRID:Addgene_121855 Copy
Species: Synthetic
Genetic Insert: Gateway selection cassette (ccdb gene + Chlor resistance gene)
Vector Backbone Description: Backbone Marker:Newgard Lab; Backbone Size:4007; Vector Backbone:JK104; Vector Types:Mammalian Expression, pMVP Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30590691
Proper citation: RRID:Addgene_121856 Copy
Species: Synthetic
Genetic Insert: Gateway selection cassette (ccdb gene + Chlor resistance gene)
Vector Backbone Description: Backbone Marker:Newgard Lab; Backbone Size:4445; Vector Backbone:JK104; Vector Types:Mammalian Expression, pMVP Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30590691
Proper citation: RRID:Addgene_121858 Copy
Species: Synthetic
Genetic Insert: Gateway selection cassette (ccdb gene + Chlor resistance gene)
Vector Backbone Description: Backbone Marker:Newgard Lab; Backbone Size:7007; Vector Backbone:IY601; Vector Types:Lentiviral, pMVP Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30590691
Comments: 3rd generation lentivirus vector
Proper citation: RRID:Addgene_121850 Copy
Species: Synthetic
Genetic Insert: Gateway selection cassette (ccdb gene + Chlor resistance gene)
Vector Backbone Description: Backbone Marker:Newgard Lab; Backbone Size:2677; Vector Backbone:IR301; Vector Types:Mammalian Expression, pMVP Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30590691
Proper citation: RRID:Addgene_121851 Copy
Species: Synthetic
Genetic Insert: Gateway selection cassette (ccdb gene + Chlor resistance gene)
Vector Backbone Description: Backbone Marker:Newgard Lab; Backbone Size:4127; Vector Backbone:JK104; Vector Types:Mammalian Expression, pMVP Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30590691
Proper citation: RRID:Addgene_121852 Copy
Species: Synthetic
Genetic Insert: Gateway selection cassette (ccdb gene + Chlor resistance gene)
Vector Backbone Description: Backbone Marker:Newgard Lab; Backbone Size:4120; Vector Backbone:JK104; Vector Types:Mammalian Expression, pMVP Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30590691
Proper citation: RRID:Addgene_121853 Copy
Species: Synthetic
Genetic Insert: Gateway selection cassette (ccdb gene + Chlor resistance gene)
Vector Backbone Description: Backbone Marker:Newgard Lab; Backbone Size:4127; Vector Backbone:JK104; Vector Types:Mammalian Expression, pMVP Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30590691
Proper citation: RRID:Addgene_121854 Copy
Species: Synthetic
Genetic Insert: Gateway selection cassette (ccdb gene + Chlor resistance gene)
Vector Backbone Description: Backbone Marker:Newgard Lab; Backbone Size:32643; Vector Backbone:IQ013; Vector Types:Adenoviral, pMVP Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30590691
Proper citation: RRID:Addgene_121844 Copy
Species: Synthetic
Genetic Insert: Gateway selection cassette (ccdb gene + Chlor resistance gene)
Vector Backbone Description: Backbone Marker:Newgard Lab; Backbone Size:32694; Vector Backbone:IQ013; Vector Types:Adenoviral, pMVP Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30590691
Proper citation: RRID:Addgene_121846 Copy
Species: Synthetic
Genetic Insert: Gateway selection cassette (ccdb gene + Chlor resistance gene)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6676; Vector Backbone:pLenti6.3/PL/V5-DEST; Vector Types:Lentiviral, pMVP Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30590691
Comments: 3rd generation lentivirus vector
Proper citation: RRID:Addgene_121849 Copy
Species: Synthetic
Genetic Insert: 3x HA epitope tag-PolyA + hU6-xCas9(3.7) gRNA scaffold
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2555; Vector Backbone:pDONR221 P3-P2; Vector Types:Gateway Cloning, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30590691
Comments: Annealed oligonucleotides containing the protospacer motif can be ligated into BsaI digested plasmid. 3x HA tag + bGH polyA; hU6 promoter PCR'd from Addgene 61591. x/SpCas9 gRNA scaffold derived from PMID: 26671237
Proper citation: RRID:Addgene_121842 Copy
Species: Synthetic
Genetic Insert: AkCas12b
Vector Backbone Description: Vector Backbone:BPK2013-ccdB; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30510770
Proper citation: RRID:Addgene_121950 Copy
Species: Synthetic
Genetic Insert: BsCas12b
Vector Backbone Description: Vector Backbone:BPK2013-ccdB; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30510770
Proper citation: RRID:Addgene_121952 Copy
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