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Species: Saccharomyces cerevisiae
Genetic Insert: ADE2
Vector Backbone Description: Vector Backbone:YDpL; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12898713
Comments: Plasmid M3939 (leu2::ADE2) was made by cutting YDp-L with EcoRV, and inserting a 3.6 kb BamHI (blunted with Klenow) fragment with ADE2 containing plasmid M1144.
Proper citation: RRID:Addgene_51672 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ADE2
Vector Backbone Description: Vector Backbone:YDpW; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12898713
Comments: Plasmid M3938 (trp1::ADE2 ) was made by cutting YDp-W with EcoRV and inserting a 3.6 kb BamHI (blunted with Klenow) fragment containing ADE2 from plasmid M1144.
Proper citation: RRID:Addgene_51673 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HIS3
Vector Backbone Description: Vector Backbone:YDp-K; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12898713
Comments: Plasmid D588 (lys2::HIS3 ) was constructed in two steps. First, plasmid YDp-K was cut with BglII and XhoI and ligated, deleting a 2477 bp fragment inside the LYS2 gene. This plasmid was then cleaved with BglII and a 1.2 kb BamHI fragment from YDp-H containing HIS3 was inserted, creating D558.
Proper citation: RRID:Addgene_51675 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LYS2
Vector Backbone Description: Vector Backbone:pIC19H–SXL2; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12898713
Comments: Plasmid D771 (leu2::LYS2) was constructed in two steps. First, plasmid YDp-K was cut with SalI and an EcoRI linker was inserted, constructing plasmid D764. Next, plasmid pIC19H–SXL2 was cut with EcoRI and a 5 kb EcoRI fragment containing LYS2 from plasmid D764 was inserted, creating D771.
Proper citation: RRID:Addgene_51677 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ADE2
Vector Backbone Description: Vector Backbone:M4757 (kanMX::TRP1), Addgene plasmid # 51687; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12898713
Comments: M4753 (kanMX::ADE2) was made by digesting M4757 with KpnI and SalI and inserting a 2.3 kb KpnI–SalI fragment with ADE2 from plasmid pFA6–ADE2.
Proper citation: RRID:Addgene_51683 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HIS3
Vector Backbone Description: Vector Backbone:M4757 (kanMX::TRP1), Addgene plasmid #51687; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12898713
Comments: M4754 (kanMX::HIS3) was made by digesting M4757 with XmaI and SalI and inserting a 1.2 kb XmaI–SalI fragment with HIS3 from plasmid YDp-H.
Proper citation: RRID:Addgene_51684 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TRP1
Vector Backbone Description: Vector Backbone:pAG25 (Goldstein and McCusker, 1999); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12898713
Comments: First, plasmid pAG25 (Goldstein and McCusker, 1999) was digested with HindIII and PacI, treated with Klenow and ligated closed. This plasmid was digested with KpnI and SphI and the 0.9 kb KpnI–SphI fragment containing TRP1 from pJJ246 was inserted, constructing M4757.
Proper citation: RRID:Addgene_51687 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: URA3
Vector Backbone Description: Vector Backbone:M4757 (kanMX::TRP1), Addgene plasmid #51687; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12898713
Comments: M4758 (kanMX::URA3) was made by digesting M4757 with BamHI and SalI and inserting a 1.2 kb BamHI–XhoI fragment with URA3 from plasmid M522.
Proper citation: RRID:Addgene_51688 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: hisG-URA3-hisG
Vector Backbone Description: Vector Backbone:M4757 (kanMX::TRP1), Addgene plasmid #51687; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12898713
Comments: M4786 (kanMX::hisG-URA3-hisG) was made by digesting M4757 with BamHI and SalI and inserting a 3.9 kb BamHI–SalI fragment with hisG–URA3–hisG from plasmid M2524.
The kanMX::hisG–URA3–hisG converter is recyclable, so that any MX-based disruption can be reverted to an unmarked disruption, allowing reuse of markers.
Proper citation: RRID:Addgene_51690 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HST2
Vector Backbone Description: Vector Backbone:p413TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19390637
Proper citation: RRID:Addgene_51745 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sir2
Vector Backbone Description: Vector Backbone:p413TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19390637
Proper citation: RRID:Addgene_51744 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sir2
Vector Backbone Description: Vector Backbone:p413TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19390637
Proper citation: RRID:Addgene_51743 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: URA3 3' fragment
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:2961; Vector Backbone:pBluescript II SK (-); Vector Types:Routine cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24604451
Comments: pJH131 was created by cloning an NheI/SacII GAL10/GAL1 promoter fragment into the same sites of pJH124. The cloned insert was confirmed by DNA sequencing.
Proper citation: RRID:Addgene_48260 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: dCas9_VP64 (codon-optimized for expression in S. cerevisiae)
Vector Backbone Description: Vector Backbone:pTPGI (pRS304); Vector Types:Yeast Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23977949
Proper citation: RRID:Addgene_49013 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pRNR2-AmtR-NES-TIR1-U1-tADH1
Vector Backbone Description: Vector Backbone:pYTK095; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38079574
Proper citation: RRID:Addgene_233857 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pTDH3-NLS-VP48-2XOaf1-DNCR2-tENO1
Vector Backbone Description: Vector Backbone:pYTK095; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38079574
Proper citation: RRID:Addgene_233854 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: lexAO6-pLEU2m-mNeonGreen-tENO2
Vector Backbone Description: Vector Backbone:pYTK095; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38079574
Proper citation: RRID:Addgene_233855 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: amtRO7-pLEU2m-mNeonGreen-tENO2
Vector Backbone Description: Vector Backbone:pYTK095; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38079574
Proper citation: RRID:Addgene_233850 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pTDH3-LexA-NLS-NS3-V1-VP48-2XOaf1-tADH1
Vector Backbone Description: Vector Backbone:pYTK095; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38079574
Proper citation: RRID:Addgene_233849 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pTDH3-rtTA3-tADH1
Vector Backbone Description: Vector Backbone:pYTK095; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38079574
Proper citation: RRID:Addgene_233842 Copy
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