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Species: Mus musculus
Genetic Insert: PECAM promoter/enhancer
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4500; Vector Backbone:EGFP-1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17068148
Proper citation: RRID:Addgene_14689 Copy
Species: Rattus norvegicus
Genetic Insert: beta arrestin 1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9924018
Proper citation: RRID:Addgene_14687 Copy
Species: Rattus norvegicus
Genetic Insert: beta-arrestin 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10097102
Proper citation: RRID:Addgene_14685 Copy
Species: Mus musculus
Genetic Insert: PECAM promoter/enhancer
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5800; Vector Backbone:EGFP-1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:14982871
Proper citation: RRID:Addgene_14686 Copy
Species: Homo sapiens
Genetic Insert: B1AR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10535961
Comments: Addgene Sanger sequencing found G389R in the B1AR translation
Proper citation: RRID:Addgene_14698 Copy
Species: Bos taurus
Genetic Insert: BARK1 minigene
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8119963
Comments: The cDNA encoding bovine BARK1 residues Gly495 to Leu689 was amplified using PCR to construct a minigene casette with newly engineered 5' and 3' ends (see Author's map). The 5' end contained an EcoRI site for subcloning, followed by the kozak sequence 5'-GCCGCCACCATGG-3'. A bclI site was inserted at the 3' end immediately following the stop codon. The final BARK1 minigene construct contains the 3'UTR of the human beta-globin gene and a polyadenylation signal.
Proper citation: RRID:Addgene_14695 Copy
Species: Rattus norvegicus
Genetic Insert: beta arrestin 1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9924018
Proper citation: RRID:Addgene_14693 Copy
Species: Homo sapiens
Genetic Insert: MKK3(ala)
Vector Backbone Description: Backbone Size:5224; Vector Backbone:pRc/RSV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8622669
Proper citation: RRID:Addgene_14669 Copy
Species: Mus musculus
Genetic Insert: p97
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:3300; Vector Backbone:pQE9; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11740563
Proper citation: RRID:Addgene_14667 Copy
Species: Homo sapiens
Genetic Insert: V1H
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7500; Vector Backbone:pACT2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12058068
Comments: For two-hybrid assay.
Proper citation: RRID:Addgene_14665 Copy
Species: Mus musculus
Genetic Insert: p97
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:3300; Vector Backbone:pQE9; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10811609
Comments: Addgene NGS results identified a V206 variant in p97 that matches NCBI reference CAA78412.1.
Proper citation: RRID:Addgene_14666 Copy
Species: Mus musculus
Genetic Insert: Myf5
Vector Backbone Description: Backbone Size:5300; Vector Backbone:EMSV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10733231
Proper citation: RRID:Addgene_14711 Copy
Species: Mus musculus
Genetic Insert: MyoD
Vector Backbone Description: Backbone Size:5300; Vector Backbone:EMSV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10733231
Proper citation: RRID:Addgene_14710 Copy
Species: Homo sapiens
Genetic Insert: MKK3
Vector Backbone Description: Backbone Size:5224; Vector Backbone:pRc/RSV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7839144
Proper citation: RRID:Addgene_14671 Copy
Species: Mus musculus
Genetic Insert: ZP1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2961; Vector Backbone:pBluescript KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7635043
Proper citation: RRID:Addgene_14644 Copy
Species: HIV
Genetic Insert: Tat
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pcDNAI/Amp; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9121429
Comments: Mutant Tat gene: C30G is in the activation domain of Tat and renders Tat inactive without affecting its RNA-binding ability or protein expression levels.
Proper citation: RRID:Addgene_14656 Copy
Vector Backbone Description: Backbone Size:8174; Vector Backbone:pLKO.3G; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Comments: A variant of pLKO.1 containing an eGFP marker rather than puromycin. Please note that the AgeI site is NOT unique for this vector, so shRNAs should be designed for cloning with EcoRI and PacI instead. Forward oligo: 5'-AATT—21bp sense—CTCGAG—21bp antisense—TTTTTTTAT-3'. Reverse oligo: 5'-AAAAAAA—21bp sense—CTCGAG—21bp antisense-3'.
pLKO.3G was cloned by excising PAC-GFP from pLKO.3pgw and replacing it with eGFP from another vector using the enzymes BamHI and BsRGI.
Proper citation: RRID:Addgene_14748 Copy
Genetic Insert: membrane bound form of EGFP
Vector Backbone Description: Backbone Size:6122; Vector Backbone:pCAGEN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17209010
Comments: mGFP is a membrane-bound form of GFP containing a palmitoylation sequence of GAP43 at its N-terminus.
The mGFP insert contains V163A and S175G compared to EGFP. These mutations are not known to affect plasmid function. The Cepko lab and several other Addgene users have used this plasmid successfully in experiments.
Addgene's NGS finds an IS4-like element (ISVsa5 family transposase) in the backbone that does not affect the overall plasmid function.
Proper citation: RRID:Addgene_14757 Copy
Species: Homo sapiens
Genetic Insert: GSK3 beta K85A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8382613
Comments: Nru I site is added to the K->A mutation
Proper citation: RRID:Addgene_14755 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Flpe-ERT2 fusion protein
Vector Backbone Description: Backbone Size:4779; Vector Backbone:pCAGEN; Vector Types:Mammalian Expression, Flp/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17209010
Comments: Kozak consensus sequence was added before the start ATG. Internal EcoRI site was destroyed by introducing a silent mutation.
Proper citation: RRID:Addgene_14756 Copy
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