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Species: Other
Genetic Insert: 6His-TEV-TBP6.7(T50A)
Vector Backbone Description: Backbone Size:5277; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29961805
Proper citation: RRID:Addgene_112727 Copy
Species: Other
Genetic Insert: 6His-TEV-TBP6.7(Q48T)
Vector Backbone Description: Backbone Size:5277; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29961805
Proper citation: RRID:Addgene_112725 Copy
Species: Other
Genetic Insert: 6His-TEV-TBP6.7(R52A)
Vector Backbone Description: Backbone Size:5277; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29961805
Proper citation: RRID:Addgene_112729 Copy
Species: Other
Genetic Insert: stRBS-lacZ
Vector Backbone Description: Backbone Marker:Joanna Goldberg (Addgene plasmid #110559); Backbone Size:6700; Vector Backbone:pJM220 (Addgene plasmid #110559); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27613678
Proper citation: RRID:Addgene_110560 Copy
Species: Other
Genetic Insert: stRBS-aacC1
Vector Backbone Description: Backbone Marker:Joanna Goldberg (Addgene plasmid #110559); Backbone Size:6700; Vector Backbone:pJM220 (Addgene plasmid #110559); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27613678
Proper citation: RRID:Addgene_110561 Copy
Species: Other
Genetic Insert: firefly Luciferase
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_111216 Copy
Species: Other
Genetic Insert: ECFP-Xpress tag-GFP10C fusion
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11071889
Proper citation: RRID:Addgene_111204 Copy
Species: Other
Genetic Insert: Leu-tRNA synthetase
Vector Backbone Description: Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29131146
Comments: Gene comes from E.coli.
Proper citation: RRID:Addgene_111480 Copy
Species: Other
Genetic Insert: Trp-tRNA synthetase
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29131146
Comments: Gene comes from E.coli.
Proper citation: RRID:Addgene_111483 Copy
Species: Other
Genetic Insert: Glu-tRNA synthetase
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29131146
Comments: Gene comes from E.coli.
Proper citation: RRID:Addgene_111473 Copy
Species: Other
Genetic Insert: translational initiation factor 2
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29131146
Comments: Gene comes from E.coli.
Proper citation: RRID:Addgene_111471 Copy
Species: Other
Genetic Insert: Asn-tRNA synthetase
Vector Backbone Description: Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29131146
Comments: Gene comes from E.coli.
Proper citation: RRID:Addgene_111477 Copy
Species: Other
Genetic Insert: translational release factor 1
Vector Backbone Description: Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29131146
Comments: Gene comes from E.coli.
Proper citation: RRID:Addgene_111478 Copy
Species: Other
Genetic Insert: translational elongation factor Ts
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29131146
Comments: Gene comes from E.coli.
Proper citation: RRID:Addgene_111467 Copy
Species: Other
Genetic Insert: Ile-tRNA synthetase
Vector Backbone Description: Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29131146
Comments: Gene comes from E.coli.
Proper citation: RRID:Addgene_111468 Copy
Species: Other
Genetic Insert: PuroR
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: Puromycin resistance gene ecnoding a puromycin N-acetyl-transferase.
Proper citation: RRID:Addgene_100682 Copy
Species: Other
Genetic Insert: SV40-NLS
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: N-terminal nuclear localization signal of SV40 large T antigen. Peptide tag for targeting the protein to the nucleus.
Proper citation: RRID:Addgene_100684 Copy
Species: Other
Genetic Insert: IRES2
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: IRES - internal ribosome entry site. ATG in included
Proper citation: RRID:Addgene_100702 Copy
Species: Other
Genetic Insert: Lentiviral 3' LTR
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: 3'LTR for lentivial vectors. The 3'LTR included the�woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) which stabilised the RNA, and the�self-inactivating 3' long terminal repeat (LTR) from HIV-1 (3' LTR (?U3))
IMPORT NOTES: Special character found in field(Comments)
Proper citation: RRID:Addgene_100703 Copy
Species: Other
Genetic Insert: SV40-ORI
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: SV40 origin of replication.
Proper citation: RRID:Addgene_100679 Copy
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