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Species: Saccharomyces cerevisiae
Genetic Insert: VHT1 gRNA
Vector Backbone Description: Backbone Marker:Laughery et al, 2015, Yeast; Backbone Size:12367; Vector Backbone:pML107; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41775267
Proper citation: RRID:Addgene_232898 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ADE2 gRNA and repair template
Vector Backbone Description: Vector Backbone:pCRISPEY-GAL-Nat; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40758833
Comments: Gibson cloning at NotI sites . Please visit https://doi.org/10.1101/2024.08.06.606807 for bioRxiv preprint.
Proper citation: RRID:Addgene_232103 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: AVO1 gRNA
Vector Backbone Description: Backbone Marker:Laughery et al, 2015, Yeast; Backbone Size:12367; Vector Backbone:pML107; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41775267
Proper citation: RRID:Addgene_232889 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Rat1
Vector Backbone Description: Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37683646
Proper citation: RRID:Addgene_231650 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ADE2 gRNA and repair template
Vector Backbone Description: Vector Backbone:pCRISPEY-Z3-Nat; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40758833
Comments: Gibson cloning at XhoI sites . Please visit https://doi.org/10.1101/2024.08.06.606807 for bioRxiv preprint.
Proper citation: RRID:Addgene_232106 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ADE2 gRNA and repair template
Vector Backbone Description: Vector Backbone:pCRISPEY-GAL-Kan; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40758833
Comments: Gibson cloning at NotI sites . Please visit https://doi.org/10.1101/2024.08.06.606807 for bioRxiv preprint.
Proper citation: RRID:Addgene_232105 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ADE2 gRNA and repair template
Vector Backbone Description: Vector Backbone:pCRISPEY-GAL-Hyg; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40758833
Comments: Gibson cloning at NotI sites . Please visit https://doi.org/10.1101/2024.08.06.606807 for bioRxiv preprint.
Proper citation: RRID:Addgene_232104 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: spt5
Vector Backbone Description: Vector Backbone:pLIB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37683646
Proper citation: RRID:Addgene_231648 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: URA3 gRNA
Vector Backbone Description: Backbone Marker:Patricia Wittkopp; Backbone Size:11961; Vector Backbone:pML104-HygMX4; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41775267
Proper citation: RRID:Addgene_232885 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RER2 gRNA
Vector Backbone Description: Backbone Marker:Laughery et al, 2015, Yeast; Backbone Size:12368; Vector Backbone:pML107; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41775267
Proper citation: RRID:Addgene_232884 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LEU2 gRNA
Vector Backbone Description: Backbone Marker:Laughery et al, 2015, Yeast; Backbone Size:11240; Vector Backbone:pML104; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41775267
Proper citation: RRID:Addgene_232894 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ScChd1 (aa118-1274)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6000; Vector Backbone:pDEST17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35173352
Proper citation: RRID:Addgene_221197 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ScChd1 (aa142-1274)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6000; Vector Backbone:pDEST17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35173352
Proper citation: RRID:Addgene_221199 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YPL264C
Vector Backbone Description: Backbone Size:3099; Vector Backbone:pNB1u/pLIFE016; Vector Types:mRNA expression, injection into xenopus oocytes; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38680917
Comments: Generated by USER cloning
Proper citation: RRID:Addgene_175230 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YBR287W
Vector Backbone Description: Backbone Size:3099; Vector Backbone:pNB1u/pLIFE016; Vector Types:mRNA expression, injection into xenopus oocytes; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38680917
Comments: Generated by USER cloning.
Proper citation: RRID:Addgene_175205 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YCR075C
Vector Backbone Description: Backbone Size:3099; Vector Backbone:pNB1u/pLIFE016; Vector Types:mRNA expression, injection into xenopus oocytes; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38680917
Comments: Generated by USER cloning
Proper citation: RRID:Addgene_175207 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YER039C
Vector Backbone Description: Backbone Size:3099; Vector Backbone:pNB1u/pLIFE016; Vector Types:mRNA expression, injection into xenopus oocytes; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38680917
Comments: Generated by USER cloning
Proper citation: RRID:Addgene_175209 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Thermosensitive fusion of I-OnuI and the L212P VMA1 intein.
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:3002; Vector Backbone:pACYC177; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38180814
Comments: The Edgell Lab uses EPI300 E. coli for transformation, but DH5alpha will also be fine.
Proper citation: RRID:Addgene_207883 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YOR307C
Vector Backbone Description: Backbone Size:3099; Vector Backbone:pNB1u/pLIFE016; Vector Types:mRNA expression, injection into xenopus oocytes; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38680917
Comments: Generated by USER cloning
Proper citation: RRID:Addgene_175227 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YPL189W
Vector Backbone Description: Backbone Size:3099; Vector Backbone:pNB1u/pLIFE016; Vector Types:mRNA expression, injection into xenopus oocytes; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38680917
Comments: Generated by USER cloning
Proper citation: RRID:Addgene_175228 Copy
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