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  • Organism:saccharomyces cerevisiae (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pML107-VHT1
 
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RRID:Addgene_232898 VHT1 gRNA Saccharomyces cerevisiae Ampicillin PMID:41775267 Backbone Marker:Laughery et al, 2015, Yeast; Backbone Size:12367; Vector Backbone:pML107; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:31:58 0
pCRISPEY-GAL-Nat-ADE2
 
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1+ mentions
RRID:Addgene_232103 ADE2 gRNA and repair template Saccharomyces cerevisiae Ampicillin PMID:40758833 Gibson cloning at NotI sites . Please visit https://doi.org/10.1101/2024.08.06.606807 for bioRxiv preprint. Vector Backbone:pCRISPEY-GAL-Nat; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin Repair template introduces C at nt 466 of ADE2 to create frameshift mutation 2026-08-15 01:31:57 1
pML107-AVO1
 
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RRID:Addgene_232889 AVO1 gRNA Saccharomyces cerevisiae Ampicillin PMID:41775267 Backbone Marker:Laughery et al, 2015, Yeast; Backbone Size:12367; Vector Backbone:pML107; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:31:58 0
pZH81 pET21b Rat1-His
 
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RRID:Addgene_231650 Rat1 Saccharomyces cerevisiae Ampicillin PMID:37683646 Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:31:58 0
pCRISPEY-Z3-Nat-ADE2
 
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1+ mentions
RRID:Addgene_232106 ADE2 gRNA and repair template Saccharomyces cerevisiae Ampicillin PMID:40758833 Gibson cloning at XhoI sites . Please visit https://doi.org/10.1101/2024.08.06.606807 for bioRxiv preprint. Vector Backbone:pCRISPEY-Z3-Nat; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin Repair template introduces C at nt 466 of ADE2 to create frameshift mutation 2026-08-15 01:31:58 1
pCRISPEY-GAL-Kan-ADE2
 
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1+ mentions
RRID:Addgene_232105 ADE2 gRNA and repair template Saccharomyces cerevisiae Ampicillin PMID:40758833 Gibson cloning at NotI sites . Please visit https://doi.org/10.1101/2024.08.06.606807 for bioRxiv preprint. Vector Backbone:pCRISPEY-GAL-Kan; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin Repair template introduces C at nt 466 of ADE2 to create frameshift mutation 2026-08-15 01:31:58 1
pCRISPEY-GAL-Hyg-ADE2
 
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1+ mentions
RRID:Addgene_232104 ADE2 gRNA and repair template Saccharomyces cerevisiae Ampicillin PMID:40758833 Gibson cloning at NotI sites . Please visit https://doi.org/10.1101/2024.08.06.606807 for bioRxiv preprint. Vector Backbone:pCRISPEY-GAL-Hyg; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin Repair template introduces C at nt 466 of ADE2 to create frameshift mutation 2026-08-15 01:31:58 1
pZH60 pLIB-spt5
 
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RRID:Addgene_231648 spt5 Saccharomyces cerevisiae Ampicillin PMID:37683646 Vector Backbone:pLIB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:31:58 0
pML104-HygMx4-URA3-2
 
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RRID:Addgene_232885 URA3 gRNA Saccharomyces cerevisiae Ampicillin PMID:41775267 Backbone Marker:Patricia Wittkopp; Backbone Size:11961; Vector Backbone:pML104-HygMX4; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:32:02 0
pML107-RER2
 
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RRID:Addgene_232884 RER2 gRNA Saccharomyces cerevisiae Ampicillin PMID:41775267 Backbone Marker:Laughery et al, 2015, Yeast; Backbone Size:12368; Vector Backbone:pML107; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:32:02 0
pML104-LEU2-3
 
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RRID:Addgene_232894 LEU2 gRNA Saccharomyces cerevisiae Ampicillin PMID:41775267 Backbone Marker:Laughery et al, 2015, Yeast; Backbone Size:11240; Vector Backbone:pML104; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:32:02 0
pDEST17-ScChd1[R126A/R130A]
 
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RRID:Addgene_221197 ScChd1 (aa118-1274) Saccharomyces cerevisiae Ampicillin PMID:35173352 Backbone Marker:Invitrogen; Backbone Size:6000; Vector Backbone:pDEST17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin R126A, R130A 2026-08-15 01:30:16 0
pDEST17-ScChd1[∆ChEx]
 
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RRID:Addgene_221199 ScChd1 (aa142-1274) Saccharomyces cerevisiae Ampicillin PMID:35173352 Backbone Marker:Invitrogen; Backbone Size:6000; Vector Backbone:pDEST17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin deleted amino acids 1-117 and 1275-1468 2026-08-15 01:30:16 0
pCfB8592
 
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RRID:Addgene_175230 YPL264C Saccharomyces cerevisiae Ampicillin PMID:38680917 Generated by USER cloning Backbone Size:3099; Vector Backbone:pNB1u/pLIFE016; Vector Types:mRNA expression, injection into xenopus oocytes; Bacterial Resistance:Ampicillin 2026-08-15 01:30:26 0
pCfB8154
 
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RRID:Addgene_175205 YBR287W Saccharomyces cerevisiae Ampicillin PMID:38680917 Generated by USER cloning. Backbone Size:3099; Vector Backbone:pNB1u/pLIFE016; Vector Types:mRNA expression, injection into xenopus oocytes; Bacterial Resistance:Ampicillin 2026-08-15 01:30:25 0
pCFB8455
 
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RRID:Addgene_175207 YCR075C Saccharomyces cerevisiae Ampicillin PMID:38680917 Generated by USER cloning Backbone Size:3099; Vector Backbone:pNB1u/pLIFE016; Vector Types:mRNA expression, injection into xenopus oocytes; Bacterial Resistance:Ampicillin 2026-08-15 01:30:25 0
pCFB8457
 
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RRID:Addgene_175209 YER039C Saccharomyces cerevisiae Ampicillin PMID:38680917 Generated by USER cloning Backbone Size:3099; Vector Backbone:pNB1u/pLIFE016; Vector Types:mRNA expression, injection into xenopus oocytes; Bacterial Resistance:Ampicillin 2026-08-15 01:30:25 0
pDual
 
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RRID:Addgene_207883 Thermosensitive fusion of I-OnuI and the L212P VMA1 intein. Saccharomyces cerevisiae Ampicillin PMID:38180814 The Edgell Lab uses EPI300 E. coli for transformation, but DH5alpha will also be fine. Backbone Marker:New England Biolabs; Backbone Size:3002; Vector Backbone:pACYC177; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin Leucine 212 changed to proline, leucine 434 in the full-length fusion. Alanine 640 changed to valine. 2026-08-15 01:30:26 0
pCfB8590
 
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RRID:Addgene_175227 YOR307C Saccharomyces cerevisiae Ampicillin PMID:38680917 Generated by USER cloning Backbone Size:3099; Vector Backbone:pNB1u/pLIFE016; Vector Types:mRNA expression, injection into xenopus oocytes; Bacterial Resistance:Ampicillin 2026-08-15 01:30:26 0
pCfB8738
 
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RRID:Addgene_175228 YPL189W Saccharomyces cerevisiae Ampicillin PMID:38680917 Generated by USER cloning Backbone Size:3099; Vector Backbone:pNB1u/pLIFE016; Vector Types:mRNA expression, injection into xenopus oocytes; Bacterial Resistance:Ampicillin 2026-08-15 01:30:26 0

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