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Vector Backbone Description: Backbone Size:8846; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1995. Fire Lab Miniprep Number pPD95.16, Ligation number NONE.
Proper citation: RRID:Addgene_1483 Copy
Species: Mus musculus
Genetic Insert: Smad7
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pBabe puro 3; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10791980
Comments: See Author's map for more information.
Proper citation: RRID:Addgene_14836 Copy
Species: Mus musculus
Genetic Insert: K-ras 3'UTR
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4045; Vector Backbone:pRL-TK; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17401365
Proper citation: RRID:Addgene_14804 Copy
Species: Rattus norvegicus
Genetic Insert: DAG reporter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12782683
Comments: CFP and YFP fused to the C1A and C1B domains of PKCbetaII. DAGR was created by PCR of the C1A and C1B domains of rat PKCßII (aa 37–152) from KpnI to XhoI and substitution for the PH domain in CYPHR.
Proper citation: RRID:Addgene_14865 Copy
Genetic Insert: C kinase activity reporter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12782683
Comments: The MyrPalm tag in this plasmid uses the Lyn kinase amino terminal sequence of MGCIKSK from UniProtKB reference sequence P07948.3. This sequence encodes for myristoylation and palmitoylation, shown previously to be sufficient to target a protein to the plasma membrane.
Proper citation: RRID:Addgene_14862 Copy
Genetic Insert: C kinase activity reporter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12782683
Comments: PKC substrate sequence flanked by flexible linker sequence: GGSGG RFRRFQTLKIKAKA GGSGG. A206K mutation present in both CFP (mCFP) and citrine (mYFP) to reduce the intrinsic homoaffinity of all GFPs and preclude intermolecular FRET by CFP-YFP dimerization. CFP was amplified by PCR from a plasmid template to encode a HindIII restriction site followed by a consensus initiation site for translation (CGCCACC) before the initiating ATG of CFP, and a KpnI restriction site at the 3' end instead of a terminating codon. FHA2, a gift from Michael Yaffe (MIT, Cambridge, MA) was amplified by PCR to include KpnI and BamHI restriction sites at the 5' and 3' ends, respectively. Citrine was amplified by PCR to include a 5' BamHI followed by the PKC substrate sequence and a 3' XbaI following the terminating codon.
Proper citation: RRID:Addgene_14860 Copy
Genetic Insert: C kinase activity reporter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16901905
Comments: NucCKAR was generated by fusing sequences encoding a basic nuclear localization signal, PKKRKVEDA, to the 3'-end of CKAR .
Proper citation: RRID:Addgene_14869 Copy
Genetic Insert: B kinase activity reporter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15583002
Comments: PKB substrate sequence: RKRDRLGTLGI.
Proper citation: RRID:Addgene_14877 Copy
Species: Rattus norvegicus
Genetic Insert: DAG binding domain
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16901905
Comments: YFP-tagged DAG binding domain (YFP-DBD) was first constructed by fusion of monomeric YFP to the 5'-end of the C1b domain of PKCbeta. This construct was improved for DAG binding by introducing the Y123W point mutation via QuikChange (Stratagene).
Proper citation: RRID:Addgene_14874 Copy
Genetic Insert: CFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16901905
Comments: Sequences encoding the amino-terminal 35 residues of endothelial nitric-oxide synthase were fused in frame to the 5'-end of CFP.
Proper citation: RRID:Addgene_14873 Copy
Genetic Insert: C kinase activity reporter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16901905
Comments: CytoCKAR was generated by fusing a region encoding the nuclear export sequence, LALKLAGLDI, to the 3'-end of CKAR immediately preceding the termination codon.
Proper citation: RRID:Addgene_14870 Copy
Species: Tobacco Rattle Virus RNA 1
Genetic Insert: TRV RNA1
Vector Backbone Description: Backbone Size:11498; Vector Backbone:pBIN19; Vector Types:Plant vector with TRV RNA1; this vector used with pYL156(TRV RNA2) for virus induced gene silencing (VIGS); Bacterial Resistance:Kanamycin
Defining Citation: PMID:12028572
Proper citation: RRID:Addgene_148968 Copy
Species: Modified Tobacco Rattle Virus RNA 2
Genetic Insert: Modified TRV RNA2
Vector Backbone Description: Backbone Size:6426; Vector Backbone:pCAMBIA0390 T-DNA vector; Vector Types:T-DNA vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12028572
Comments: There are a few differences between the Addgene full sequence and the Genbank ID: AF406991. These discrepancies were confirmed to not cause functional differences:
- 1 bp insertion (T3313) in backbone.
- 1 bp mismatch (T5593C) and 1 bp insertion (G5646) in pVS1 RepA (known variant).
- IS4-like element inserted between ori and KanR (1338bp insert at positions 8295-9632bp).
Proper citation: RRID:Addgene_148969 Copy
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Naldini; Backbone Size:0; Vector Backbone:LV-pGFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11854510
Comments: CAG promoter was cloned into the ClaI and BamHI sites of LV-pGFP, replacing the PGK promoter.
Proper citation: RRID:Addgene_14857 Copy
Genetic Insert: CMV-GFP
Vector Backbone Description: Backbone Size:0; Vector Backbone:pHR'; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9294208
Comments: HIV vector with CMV promoter. Constructed by inserting the CMV-GFP fragment from pKS-CMV-GFP into the same sites of pHR'.
Proper citation: RRID:Addgene_14858 Copy
Vector Backbone Description: Backbone Size:7236; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1995. Fire Lab Miniprep Number pPD95.57, Ligation number NONE.
Proper citation: RRID:Addgene_1488 Copy
Species: Homo sapiens
Genetic Insert: STRAD alpha
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5300; Vector Backbone:pcDNA4/HisMax; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14985505
Proper citation: RRID:Addgene_14889 Copy
Species: Homo sapiens
Genetic Insert: IgK-IFN
Vector Backbone Description: Backbone Size:5800; Vector Backbone:na; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12356734
Comments: Contains two copies of the immunoglobulin kappa light chain kappa-B site (5'-GGGGACTTTCC-3') upstream of the interferon-beta minimal promoter (–55 to +19) (Fujita et al., 1987) driving luciferase expression.
Proper citation: RRID:Addgene_14886 Copy
Species: Homo sapiens
Genetic Insert: miR-1
Vector Backbone Description: Backbone Marker:Bartel Lab; Backbone Size:2600; Vector Backbone:pTRE-TIGHT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14697198
Comments: Expression of a transcript behind TRE-Tight tetracycline regulatable promoter. This vector is part of the BD-Tet-ON/Tet-Off system for inducible expression in mammalian cells.
miR-1 is predicted to target Glucose-6 phosphate 1-dehydrogenase (G6PD).
The sequence provided is approximated based on the information that miR-1 was inserted at the BamHI and ClaI sites of pTRE-TIGHT. There may be slight differences in the actual sequence near the cloning sites.
Proper citation: RRID:Addgene_14896 Copy
Species: evolved fluorescent protein
Genetic Insert: pmKalama1-Nuc
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4000; Vector Backbone:pEYFP-Nuc; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17444659
Proper citation: RRID:Addgene_14894 Copy
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