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Species: Synthetic
Genetic Insert: acrosin-GFP-GPI
Vector Backbone Description: Backbone Marker:Carmen Williams; Backbone Size:3059; Vector Backbone:pIVT2; Vector Types:For in vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26623518
Proper citation: RRID:Addgene_122141 Copy
Species: Synthetic
Genetic Insert: rsCaMPARI
Vector Backbone Description: Backbone Size:4270; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32931424
Proper citation: RRID:Addgene_122092 Copy
Species: Synthetic
Genetic Insert: Chronos-tdTomato
Vector Backbone Description: Backbone Marker:Scott Sternson lab; Backbone Size:4974; Vector Backbone:pAAV-EF1α1.1; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24509633
Comments: The plasmid is fully sequenced in the coding sequence regions (opsin-fluorophore and important flanking regions). Multiple digestions were done to verify the vector structure. The construct was tested in vitro.
Proper citation: RRID:Addgene_122099 Copy
Species: Synthetic
Genetic Insert: Chronos-GFP
Vector Backbone Description: Backbone Marker:Scott Sternson lab; Backbone Size:4974; Vector Backbone:pAAV-EF1α1.1; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24509633
Comments: The plasmid is fully sequenced in the coding sequence regions (opsin-fluorophore and important flanking regions). Multiple digestions were done to verify the vector structure. The construct was tested in vitro.
Proper citation: RRID:Addgene_122098 Copy
Species: Synthetic
Genetic Insert: NmCas9 tracrRNA
Vector Backbone Description: Backbone Size:4314; Vector Backbone:pBS; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30451839
Comments: To design oligos for cloning: 5'-ACCG-Spacer_Sequence-3' and 5'-AAAC-Rev_Comp_Spacer-3'
For sgRNA cloning: Digest with BfuAI and ligate in phosphorylated and annealed oligos
Proper citation: RRID:Addgene_122091 Copy
Species: Synthetic
Genetic Insert: SpCas9 tracr
Vector Backbone Description: Backbone Size:4280; Vector Backbone:pBS; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30451839
Comments: To design oligos for cloning: 5'-ACCG-Spacer_Sequence-3' and 5'-AAAC-Rev_Comp_Spacer-3'
For sgRNA cloning: Digest with BfuAI and ligate in phosphorylated and annealed oligos
Proper citation: RRID:Addgene_122089 Copy
Species: Synthetic
Genetic Insert: ChrimsonR-tdTomato
Vector Backbone Description: Backbone Marker:Scott Sternson lab; Backbone Size:4974; Vector Backbone:pAAV-EF1α1.1; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24509633
Comments: The plasmid is fully sequenced in the coding sequence regions (opsin-fluorophore and important flanking regions). Multiple digestions were done to verify the vector structure. The construct was tested in vitro.
Proper citation: RRID:Addgene_122064 Copy
Species: Synthetic
Genetic Insert: tdTomato
Vector Backbone Description: Backbone Marker:Scott Sternson lab; Backbone Size:4980; Vector Backbone:pAAV-EF1α1.1; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24509633
Comments: The plasmid is fully sequenced in the coding sequence regions (fluorophore region). Multiple digestions were done to verify the vector structure. The construct was tested in vitro.
Proper citation: RRID:Addgene_122101 Copy
Species: Synthetic
Genetic Insert: Chronos-GFP
Vector Backbone Description: Backbone Marker:Epoch; Backbone Size:5156; Vector Backbone:AAV- EF1α1.1-FLPX; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24509633
Comments: The plasmid is fully sequenced in the coding sequence regions (opsin-fluorophore and important flanking regions). Multiple digestions were done to verify the vector structure. The construct and the virus were both tested in vitro.
Proper citation: RRID:Addgene_122102 Copy
Species: Synthetic
Genetic Insert: sgRNA for mouse Hes1
Vector Backbone Description: Backbone Size:8427; Vector Backbone:px335; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32596448
Proper citation: RRID:Addgene_122310 Copy
Species: Synthetic
Genetic Insert: Firefly luciferase
Vector Backbone Description: Backbone Size:9199; Vector Backbone:pLenti-TK-Firefly-Control; Vector Types:Mammalian Expression, Lentiviral, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31538617
Proper citation: RRID:Addgene_122278 Copy
Species: Synthetic
Genetic Insert: dCas9-KRAB-MeCP2
Vector Backbone Description: Vector Backbone:PB-TRE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.06.28.449297v5 for bioRxiv preprint.
Proper citation: RRID:Addgene_122267 Copy
Species: Synthetic
Genetic Insert: sgRNA for mouse Nanog
Vector Backbone Description: Backbone Size:8427; Vector Backbone:px335; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32596448
Proper citation: RRID:Addgene_122302 Copy
Species: Synthetic
Genetic Insert: sgRNA for mouse Slc2a3
Vector Backbone Description: Backbone Size:8427; Vector Backbone:px335; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32596448
Proper citation: RRID:Addgene_122305 Copy
Species: Synthetic
Genetic Insert: sgRNA for mouse Slc2a3
Vector Backbone Description: Backbone Size:8427; Vector Backbone:px335; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32596448
Proper citation: RRID:Addgene_122304 Copy
Species: Synthetic
Genetic Insert: gRNAwcaF159
Vector Backbone Description: Vector Backbone:pBbE2k; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30177768
Proper citation: RRID:Addgene_122259 Copy
Species: Synthetic
Genetic Insert: sgRNA with cs2 at the 3' end of the constant region
Vector Backbone Description: Vector Backbone:pU6-sgRNA EF1Alpha-puro-T2A-BFP (Addgene, #60955); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32231336
Proper citation: RRID:Addgene_122237 Copy
Species: Synthetic
Genetic Insert: sgRNA with cs1 in stem loop 2 of the constant region
Vector Backbone Description: Vector Backbone:pU6-sgRNA EF1Alpha-puro-T2A-BFP (Addgene, #60955); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32231336
Proper citation: RRID:Addgene_122238 Copy
Species: Synthetic
Genetic Insert: Firefly luciferase
Vector Backbone Description: Backbone Size:9199; Vector Backbone:pLenti-TK-Firefly-Control; Vector Types:Mammalian Expression, Lentiviral, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31538617
Proper citation: RRID:Addgene_122282 Copy
Species: Synthetic
Genetic Insert: Firefly luciferase
Vector Backbone Description: Backbone Size:9199; Vector Backbone:pLenti-TK-Firefly-Control; Vector Types:Mammalian Expression, Lentiviral, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31538617
Proper citation: RRID:Addgene_122284 Copy
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