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Species: Synthetic
Genetic Insert: Neo*
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript; Vector Types:Mammalian Expression, Bacterial Expression, Mouse Targeting, att/PhiC31; Bacterial Resistance:Ampicillin
Comments: The FRT sites in plasmid pFNF #22687 were replaced by attB and attP sites.
The Neo* coding sequence has an E to D mutation at residue 185 corresponding to the original residue 182 (see Yenofsky et al., 1990, https://doi.org/10.1073/pnas.87.9.3435). Its reduced activity could mean that the cell would have to have higher level of the mutant protein expressed in order to achieve drug resistance at the same G418 dose. This could be relevant to mouse embryonic stem cell gene targeting efficiency (also see Hanson and Sedivy, 1995, https://doi.org/10.1128/mcb.15.1.45).
Proper citation: RRID:Addgene_138412 Copy
Species: Synthetic
Genetic Insert: Himar-dCas9
Vector Backbone Description: Backbone Size:5647; Vector Backbone:pET151; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31742433
Proper citation: RRID:Addgene_138414 Copy
Species: Synthetic
Genetic Insert: Neo*
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript; Vector Types:Mammalian Expression, Bacterial Expression, Mouse Targeting, piggyBac; Bacterial Resistance:Ampicillin
Comments: The FRT sites in plasmid pFNF #22687 were replaced by 5' and 3' piggyBac ITR sequences.
The Neo* coding sequence has an E to D mutation at residue 185 corresponding to the original residue 182 (see Yenofsky et al., 1990, https://doi.org/10.1073/pnas.87.9.3435). Its reduced activity could mean that the cell would have to have higher level of the mutant protein expressed in order to achieve drug resistance at the same G418 dose. This could be relevant to mouse embryonic stem cell gene targeting efficiency (also see Hanson and Sedivy, 1995, https://doi.org/10.1128/mcb.15.1.45).
Proper citation: RRID:Addgene_138413 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Stefano Piccolo (Addgene plasmid # 84008); Backbone Size:8477; Vector Backbone:FUW-tetO-MCS; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31988126
Proper citation: RRID:Addgene_138531 Copy
Species: Synthetic
Genetic Insert: tdTomato
Vector Backbone Description: Backbone Marker:Stefano Piccolo (Addgene plasmid # 84008); Backbone Size:8477; Vector Backbone:FUW-tetO-MCS; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31988126
Proper citation: RRID:Addgene_138532 Copy
Species: Synthetic
Genetic Insert: eSpCas9(1.1)
Vector Backbone Description: Vector Backbone:pFUGW; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32514125
Proper citation: RRID:Addgene_138555 Copy
Species: Synthetic
Genetic Insert: Sniper-Cas9
Vector Backbone Description: Vector Backbone:pFUGW; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32514125
Proper citation: RRID:Addgene_138559 Copy
Species: Synthetic
Genetic Insert: VQR
Vector Backbone Description: Vector Backbone:pFUGW; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32514125
Proper citation: RRID:Addgene_138560 Copy
Species: Synthetic
Genetic Insert: QQR1
Vector Backbone Description: Vector Backbone:pFUGW; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32514125
Proper citation: RRID:Addgene_138564 Copy
Species: Synthetic
Genetic Insert: xCas9
Vector Backbone Description: Vector Backbone:pFUGW; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32514125
Proper citation: RRID:Addgene_138565 Copy
Species: Synthetic
Genetic Insert: T7-tagged streptavidin variant with native C-terminus
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET30b; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28062280
Proper citation: RRID:Addgene_138588 Copy
Species: Synthetic
Genetic Insert: PIP-FUCCI
Vector Backbone Description: Backbone Size:8000; Vector Backbone:pLenti PGK Hygro DEST (w530-1); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30421640
Proper citation: RRID:Addgene_138714 Copy
Species: Synthetic
Genetic Insert: ZF37x1
Vector Backbone Description: Vector Backbone:pPD152 Reporter Template v2; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32034124
Comments: Leonard Lab plasmid reference number: L1397
Proper citation: RRID:Addgene_138717 Copy
Species: Synthetic
Genetic Insert: ZF97x1
Vector Backbone Description: Vector Backbone:pPD152 Reporter Template v2; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32034124
Comments: Leonard Lab plasmid reference number: L1400
Proper citation: RRID:Addgene_138719 Copy
Species: Synthetic
Genetic Insert: ZF92x1
Vector Backbone Description: Vector Backbone:pPD152 Reporter Template v2; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32034124
Comments: Leonard Lab plasmid reference number: L1399
Proper citation: RRID:Addgene_138718 Copy
Species: Synthetic
Genetic Insert: ZF43x1
Vector Backbone Description: Vector Backbone:pPD630 Integration Vector; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32034124
Comments: Leonard lab plasmid reference number: L1542
Proper citation: RRID:Addgene_138740 Copy
Species: Synthetic
Genetic Insert: ZF43x3-Spaced
Vector Backbone Description: Vector Backbone:pPD630 Integration Vector; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32034124
Comments: Leonard lab plasmid reference number: L1546
Proper citation: RRID:Addgene_138744 Copy
Species: Synthetic
Genetic Insert: ZF43x12-Spaced
Vector Backbone Description: Vector Backbone:pPD630 Integration Vector; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32034124
Comments: Leonard lab plasmid reference number: L1548
Proper citation: RRID:Addgene_138746 Copy
Species: Synthetic
Genetic Insert: ZF43x6-Spaced
Vector Backbone Description: Vector Backbone:pPD630 Integration Vector; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32034124
Comments: Leonard lab plasmid reference number: L1547
Proper citation: RRID:Addgene_138745 Copy
Species: Synthetic
Genetic Insert: VP16-ZF21
Vector Backbone Description: Vector Backbone:pPD005 pcDNA Golden Gate; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32034124
Comments: Leonard Lab plasmid reference number: L1294
Proper citation: RRID:Addgene_138757 Copy
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