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Species: Streptococcus pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pB[PUb-ZsGreen]; Vector Types:Insect Expression, piggyBac; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34661429
Proper citation: RRID:Addgene_169012 Copy
Genetic Insert: Cre Recombinase
Vector Backbone Description: Backbone Marker:VectorBuilder; Backbone Size:3586; Vector Backbone:N/A; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_169013 Copy
Genetic Insert: Cre Recombinase
Vector Backbone Description: Backbone Marker:VectorBuilder; Backbone Size:5282; Vector Backbone:N/A; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_169014 Copy
Species: Homo sapiens
Genetic Insert: NFAT enhancer (from IL-4 promoter)
Vector Backbone Description: Vector Backbone:FL1.8WPREHS4; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_169096 Copy
Species: Danio rerio
Genetic Insert: BMP2b
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Probe: cut with EcoRI, transcribe with T3 RNA polymerase.
Proper citation: RRID:Addgene_16915 Copy
Species: Rattus norvegicus
Genetic Insert: microtubule-associated protein 1 light chain 3 beta
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27818143
Proper citation: RRID:Addgene_168997 Copy
Species: Rattus norvegicus
Genetic Insert: microtubule-associated protein 1 light chain 3 beta
Vector Backbone Description: Vector Backbone:pT2AL200R150G; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27818143
Proper citation: RRID:Addgene_168998 Copy
Species: Homo sapiens
Genetic Insert: PA-Rac-1
Vector Backbone Description: Backbone Marker:Chi-Bin Chien lab; Backbone Size:7796; Vector Backbone:pDestTol2CG2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32516597
Proper citation: RRID:Addgene_169044 Copy
Species: Synthetic
Genetic Insert: ReaChR-Citrine
Vector Backbone Description: Backbone Marker:Chi-Bin Chien lab; Backbone Size:7796; Vector Backbone:pDestTol2CG2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32516597
Proper citation: RRID:Addgene_169046 Copy
Species: Homo sapiens
Genetic Insert: MAP1LC3B
Vector Backbone Description: Backbone Marker:Merck; Backbone Size:5420; Vector Backbone:pETDuet1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29343546
Comments: Internal construct reference: SMC-948
Proper citation: RRID:Addgene_169168 Copy
Species: Escherichia coli and Aequorea victoria
Genetic Insert: H6-spTorA-GFP
Vector Backbone Description: Backbone Marker:Beckwith Lab; Backbone Size:4542; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34499687
Comments: The spTorA-GFP coding sequence was recovered from p-spTorA-GFP-H6C, a plasmid originally reported as pTorA-GFP in Bageshwar & Musser 2007 J. Cell Biol., 179:87-99.
Proper citation: RRID:Addgene_169041 Copy
Species: SARS-CoV-2
Genetic Insert: 3xFlag-nsp5CS[VATLQ]-nsp14
Vector Backbone Description: Vector Backbone:pET11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34198326
Comments: We have included the predicted cleavage site for nsp5 found between nsp13 and nsp14 (VATLQ) between the 3xFlag tag and nsp14 in this construct, which should allow a purified nsp5 protease to cleave-out the 3xFlag tag similarly to what might happen in cells.
Proper citation: RRID:Addgene_169159 Copy
Species: Synthetic
Genetic Insert: 3xFlag-nsp5CS[VRLQ]-nsp10
Vector Backbone Description: Vector Backbone:pET11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34198326
Comments: We have included the predicted cleavage site for nsp5 found between nsp9 and nsp10 (VRLQ) between the 3xFlag tag and nsp10 in this construct that should allow a purified nsp5 protease to cleave-out the 3xFlag tag similarly to what might happen in cells.
Proper citation: RRID:Addgene_169157 Copy
Vector Backbone Description: Backbone Size:12746; Vector Backbone:pAC; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33444322
Comments: For more information about the Han Lab Drosophila Transgenic Vectors, please visit: https://han.wicmb.cornell.edu/han-lab-drosophila-transgenic-vectors/
Proper citation: RRID:Addgene_169030 Copy
Species: Erythrobacter litoralis
Genetic Insert: VP16-EL222
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34346207
Proper citation: RRID:Addgene_169151 Copy
Species: SARS-CoV-2
Genetic Insert: 3xFlag-His-nsp5CS-nsp9
Vector Backbone Description: Vector Backbone:pLIB (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34198327
Comments: Baculovirus generation using Tn7 transposition (Bac-to-Bac).
Proper citation: RRID:Addgene_169177 Copy
Species: Mus musculus
Genetic Insert: RiboL1-jGCaMP8s
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36739289
Proper citation: RRID:Addgene_169247 Copy
Species: Homo sapiens
Genetic Insert: CDK5
Vector Backbone Description: Backbone Size:6500; Vector Backbone:pcmv; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_1692 Copy
Genetic Insert: mU6-sgRNA
Vector Backbone Description: Vector Backbone:pMJ114; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34016988
Proper citation: RRID:Addgene_169235 Copy
Species: Danio rerio
Genetic Insert: snail
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Antisense probe: cut with EcoR1 and transcribe with T3.
Sense probe: cut with BamH1 and transcribe with T7.
Plasmid History: Constructed by Greg Kelly, 1/94.
Proper citation: RRID:Addgene_16931 Copy
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