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Species: Homo sapiens
Genetic Insert: Ehmt1
Vector Backbone Description: Backbone Marker:Steve Elledge; Backbone Size:6699; Vector Backbone:pSMP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388813
Proper citation: RRID:Addgene_36336 Copy
Species: Homo sapiens
Genetic Insert: Ehmt1
Vector Backbone Description: Backbone Marker:Steve Elledge; Backbone Size:6699; Vector Backbone:pSMP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388813
Proper citation: RRID:Addgene_36335 Copy
Species: Homo sapiens
Genetic Insert: Dot1L
Vector Backbone Description: Backbone Marker:Steve Elledge; Backbone Size:6699; Vector Backbone:pSMP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388813
Proper citation: RRID:Addgene_36339 Copy
Species: Homo sapiens
Genetic Insert: Ehmt1
Vector Backbone Description: Backbone Marker:Steve Elledge; Backbone Size:6699; Vector Backbone:pSMP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388813
Proper citation: RRID:Addgene_36338 Copy
Species: Homo sapiens
Genetic Insert: Ehmt1
Vector Backbone Description: Backbone Marker:Steve Elledge; Backbone Size:6699; Vector Backbone:pSMP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388813
Comments: Addgene's quality control sequence varies slightly from the depositor's, but this plasmid should be functional.
Proper citation: RRID:Addgene_36337 Copy
Species: Homo sapiens
Genetic Insert: shRNA of beta-catenin-2
Vector Backbone Description: Backbone Marker:Biosettia; Backbone Size:10275; Vector Backbone:pLV-H1TetO-RFP-Puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22645348
Proper citation: RRID:Addgene_36299 Copy
Species: Homo sapiens
Genetic Insert: shRNA of beta-catenin-1
Vector Backbone Description: Backbone Marker:Biosettia; Backbone Size:10275; Vector Backbone:pLV-H1TetO-RFP-Puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22645348
Proper citation: RRID:Addgene_36297 Copy
Species: Homo sapiens
Genetic Insert: EZH2
Vector Backbone Description: Backbone Marker:Steve Elledge; Backbone Size:6699; Vector Backbone:pSMP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388813
Proper citation: RRID:Addgene_36389 Copy
Species: Homo sapiens
Genetic Insert: ubiquitin specific peptidase 19
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21849505
Proper citation: RRID:Addgene_36307 Copy
Species: Homo sapiens
Genetic Insert: DNMT1
Vector Backbone Description: Backbone Marker:Steve Elledge; Backbone Size:6699; Vector Backbone:pSMP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388813
Comments: Addgene's quality control sequence varies slightly from the depositor's, but this plasmid should be functional.
Proper citation: RRID:Addgene_36383 Copy
Species: Homo sapiens
Genetic Insert: EED
Vector Backbone Description: Backbone Marker:Steve Elledge; Backbone Size:6699; Vector Backbone:pSMP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388813
Proper citation: RRID:Addgene_36386 Copy
Species: Homo sapiens
Genetic Insert: DNMT1
Vector Backbone Description: Backbone Marker:Steve Elledge; Backbone Size:6699; Vector Backbone:pSMP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388813
Proper citation: RRID:Addgene_36384 Copy
Species: Homo sapiens
Genetic Insert: active BCR-related
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17116687
Comments: This is the ABR isoform A. The R683A N795A double mutant lacks GTPase activity towards RacGTP. Construct: Young-jin Cho. Also see PMID 20962234.
Proper citation: RRID:Addgene_36417 Copy
Species: Homo sapiens
Genetic Insert: breakpoint cluster region protein isoform 1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20962234
Comments: Also see PMID 17116687.
Proper citation: RRID:Addgene_36416 Copy
Species: Homo sapiens
Genetic Insert: active BCR-related
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17116687
Comments: Made by direct mutagenesis of EYFP-Abr using a Stratagene Quick Change kit. In Dbl, mutation of residues N673 and D674 completely abolished its activity as a GEF towards Rac. These residues are highly conserved among DH domains and are also conserved as residues N245/E246 in Abr. We therefore mutated these residues in Abr and introduced the mutant into the RAW 264.7 macrophage cell line. However, the construct’s expression did not cause morphological changes in these cells. constructed by Young-Jin Cho.
Proper citation: RRID:Addgene_36419 Copy
Species: Homo sapiens
Genetic Insert: breakpoint cluster region
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17116687
Comments: Made by mutagenesis directly on plasmid Bcr-ECFP using a Stratagene Quick Change kit. In Dbl, mutation of residues N673 and D674 completely abolished its activity as a GEF towards Rac. These residues are highly conserved among DH domains and are also conserved as residues N689/ E690 in Bcr. We therefore mutated these residues in Bcr and introduced the mutants into the RAW 264.7 macrophage cell line. However, the construct did not caused morphological changes in these cells. Constructed by Young-Jin Cho.
Proper citation: RRID:Addgene_36418 Copy
Species: Homo sapiens
Genetic Insert: EHD1 EH domain
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4984; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_36459 Copy
Species: Homo sapiens
Genetic Insert: FANCC-TALEN_Left
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32290); Backbone Size:6447; Vector Backbone:JDS78; Vector Types:Mammalian, T7 for in vitro mRNA synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TGAAGGGACATCACCTTT
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb.
Proper citation: RRID:Addgene_36697 Copy
Species: Homo sapiens
Genetic Insert: autophagy-related protein 2 homolog A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22219374
Proper citation: RRID:Addgene_36456 Copy
Species: Homo sapiens
Genetic Insert: BMPR1A-TALEN_Left
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32290); Backbone Size:6447; Vector Backbone:JDS78; Vector Types:Mammalian, T7 for in vitro mRNA synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TACAATTGAACAATGCCT
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb.
Proper citation: RRID:Addgene_36699 Copy
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