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Species: Synthetic
Genetic Insert: WP_119951226_1
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: pET28b_NdeIXhoI vector with WP_119951226_1 insert, JGI ICE Registry: https://registry.jgi.doe.gov/entry/18131
Proper citation: RRID:Addgene_158935 Copy
Species: Synthetic
Genetic Insert: CVN04163_1
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: pET28b_NdeIXhoI vector with CVN04163_1 insert, JGI ICE Registry: https://registry.jgi.doe.gov/entry/18151
Proper citation: RRID:Addgene_158945 Copy
Species: Synthetic
Genetic Insert: OGN97459_1
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: pET28b_NdeIXhoI vector with OGN97459_1 insert, JGI ICE Registry: https://registry.jgi.doe.gov/entry/18147
Proper citation: RRID:Addgene_158943 Copy
Species: Synthetic
Genetic Insert: WP_005508112_1
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: pET28b_NdeIXhoI vector with WP_005508112_1 insert, JGI ICE Registry: https://registry.jgi.doe.gov/entry/18149
Proper citation: RRID:Addgene_158944 Copy
Species: Synthetic
Genetic Insert: WP_061782733_1
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: pET28b_NdeIXhoI vector with WP_061782733_1 insert, JGI ICE Registry: https://registry.jgi.doe.gov/entry/18143
Proper citation: RRID:Addgene_158941 Copy
Species: Synthetic
Genetic Insert: ABL00697_1
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: pET28b_NdeIXhoI vector with ABL00697_1 insert, JGI ICE Registry: https://registry.jgi.doe.gov/entry/18141
Proper citation: RRID:Addgene_158940 Copy
Species: Synthetic
Genetic Insert: BLRR
Vector Backbone Description: Backbone Size:7983; Vector Backbone:pLenti CMV Puro DEST (w118-1); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32797168
Proper citation: RRID:Addgene_158958 Copy
Species: Synthetic
Genetic Insert: dSaCas9-VP64
Vector Backbone Description: Vector Backbone:pX601 (Addgene #61591); Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31108320
Proper citation: RRID:Addgene_158972 Copy
Species: Synthetic
Genetic Insert: gRNA3
Vector Backbone Description: Vector Backbone:pX330-U6-Chimeric_BB-CBh-hSpCas9; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32797168
Proper citation: RRID:Addgene_158973 Copy
Species: Synthetic
Genetic Insert: tdTomato
Vector Backbone Description: Backbone Marker:Andras Nagy; Vector Backbone:PB-CA; Vector Types:Mammalian Expression, piggyBac; Bacterial Resistance:Ampicillin
Comments: The tdTomato expression cassette insert is the same as in plasmid PB CAG-tdTomato (https://www.addgene.org/133569/).
Four nucleotides that were previously missing in the 5' end of the WPRE were added back, and SnapGene now recognizes it as WPRE.
Proper citation: RRID:Addgene_159095 Copy
Species: Synthetic
Genetic Insert: tSapphire
Vector Backbone Description: Vector Backbone:pFA6; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_159313 Copy
Species: Synthetic
Genetic Insert: ZFN targeting the AAVS1 locus
Vector Backbone Description: Backbone Size:2967; Vector Backbone:pCMV vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_159298 Copy
Species: Synthetic
Genetic Insert: TALEN targeting the AAVS1
Vector Backbone Description: Backbone Size:4506; Vector Backbone:pcDNA; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_159299 Copy
Species: Synthetic
Genetic Insert: ZFN targeting the AAVS1 locus
Vector Backbone Description: Backbone Size:2967; Vector Backbone:pCMV vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_159297 Copy
Species: Synthetic
Genetic Insert: SaCas9
Vector Backbone Description: Vector Backbone:pX601 (Addgene #61591); Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31108320
Proper citation: RRID:Addgene_159173 Copy
Species: Synthetic
Genetic Insert: rbsR-L
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_159358 Copy
Species: Synthetic
Genetic Insert: TAX-L10-STEPtag
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5677; Vector Backbone:pET-11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33044070
Comments: TAX can be replaced with a protein of interest using the NdeI and XhoI sites. Ensure that the flexible ten amino acid linker between STEPtag and the protein of interest is present in the new fusion.
Proper citation: RRID:Addgene_159375 Copy
Species: Synthetic
Genetic Insert: gSTEP1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5301; Vector Backbone:pET-Duet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33044070
Proper citation: RRID:Addgene_159376 Copy
Species: Synthetic
Genetic Insert: STEPtag
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5677; Vector Backbone:pET-11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33044070
Proper citation: RRID:Addgene_159373 Copy
Species: Synthetic
Genetic Insert: mRuby2
Vector Backbone Description: Vector Backbone:pFA6; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_159306 Copy
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