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Species: Homo sapiens
Genetic Insert: Smad3 (EPSM)
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:xenopus/mammalian/avian/zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10197981
Comments: 3SP and 1TP sites in the linker are mutated to alanine or valine (T-DV). See author's map for more information. Please note that the mutations correspond to residues 179, 204, 208, and 213 of the native Smad3 protein.
Proper citation: RRID:Addgene_14963 Copy
Genetic Insert: iSpyMacCas9
Vector Backbone Description: Vector Backbone:pYPQ165; Vector Types:Plant Expression, CRISPR, Gateway compatible ziSpyMacCas9 entry clone; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33898973
Proper citation: RRID:Addgene_149553 Copy
Genetic Insert: hAID-ziSpyMacCas9(D10A)-UGI
Vector Backbone Description: Vector Backbone:pNJB91; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33898973
Proper citation: RRID:Addgene_149554 Copy
Genetic Insert: ziSpyMacCas9(D10A)-PmCDA1-UGI
Vector Backbone Description: Vector Backbone:pNJB91; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33898973
Proper citation: RRID:Addgene_149555 Copy
Species: Homo sapiens
Genetic Insert: Smad1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8637600
Comments: See author's map for more information.
Proper citation: RRID:Addgene_14956 Copy
Species: Homo sapiens
Genetic Insert: Smad4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16172383
Comments: Site directed mutagenesis in the coding sequence of Tyr162 (from TAT to TAC) and Val163 (from GTG to GTT). The mutated sequence encodes the same amino acids. See author's map for more information.
Proper citation: RRID:Addgene_14959 Copy
Vector Backbone Description: Backbone Size:7562; Vector Backbone:pBigT; Vector Types:Cre/Lox, for making transgenic constructs; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14657333
Comments: Modification of plasmid pBigT described in Srinivas et al. BMC Dev Biol (2001) vol 1, p.4. For use with pRosa26PA. See BMC Dev Biol article for more information.
Modified to contain IRES-EGFP downstream of cloning site. See Author's Map for more information.
Proper citation: RRID:Addgene_15037 Copy
Species: Xenopus laevis
Genetic Insert: beta-globin 5' and 3' UTR
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pSP64; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:6207484
Comments: Vector with Xenopus 5' and 3' untranslated beta-globin sequences to enhance translation of synthetic mRNAs. Cloning site for mRNA is BglII. See author's map for more information.
Proper citation: RRID:Addgene_15030 Copy
Species: Homo sapiens
Genetic Insert: Brca1
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10550055
Comments: pBABEpuro HABrca1 was made by digesting pCDNA3beta HABrca1 (R. Scully, et al., Cell 88, 265, 1997) with SalI and XhoI and inserting it into the SalI site of pBABEpuro.
The BRCA1 insert in this plasmid contains the following common polymorphisms when compared to GenBank reference sequence NP_009225.1: P871L, E1038G, K1182R, and S1613G. These SNPs were likely present in the original cDNA isolate.
Proper citation: RRID:Addgene_14999 Copy
Species: Mus musculus
Genetic Insert: Mer
Vector Backbone Description: Backbone Marker:BD Biosciences; Backbone Size:5300; Vector Backbone:pIRES2-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15673687
Comments: The full-length mouse Mer (994 amino acids) was cloned from a 16-day-old mouse embryo cDNA library. Addgene's quality control found the closest match to the insert sequence is mouse Mer (AA17-994) Sequence ID: NP_032613.1, with mutations I516V and Q860R. The plasmid should function as described in the associated article listed on the plasmid page above.
Proper citation: RRID:Addgene_14998 Copy
Species: Homo sapiens
Genetic Insert: Smad3
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12150994
Comments: See author's map for more information.
Proper citation: RRID:Addgene_14966 Copy
Species: Homo sapiens
Genetic Insert: Smad3
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12150994
Comments: See author's map for more information.
Proper citation: RRID:Addgene_14967 Copy
Species: Homo sapiens
Genetic Insert: c-myc promoter (-109/+334)
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pBV-luciferase; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11158583
Comments: XhoI to PvuII of c-myc promoter was subcloned into pBV-Luc, a low basal activity luciferase reporter plasmid.
Proper citation: RRID:Addgene_14969 Copy
Genetic Insert: Cre-ER(TM)
Vector Backbone Description: Backbone Size:3000; Vector Backbone:modified pBluescript II KS; Vector Types:Mammalian Expression, Cre/Lox, for making transgenic constructs; Bacterial Resistance:Ampicillin
Comments: The CRE-ER(TM) protein requires tamoxifen (TM) to catalyze LoxP site-mediated excision.
See Author's Map for more information.
Proper citation: RRID:Addgene_15028 Copy
Species: Mus musculus
Genetic Insert: Pdx1 promoter
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Mammalian Expression, Cre/Lox, mouse transgenic; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11973276
Comments: To generate the Pdx1-Cre construct, a 5.5 kb SalI-SmaI fragment (blunt-ended) containing the Pdx1 promoter from pKSpdx-1SalI was ligated to the XbaI-digested (blunt-ended) pMC-Cre. The insert was released by SalI and NotI digestion.
See Author's Map for more information.
Proper citation: RRID:Addgene_15021 Copy
Species: Homo sapiens
Genetic Insert: Angiotensin-converting enzyme 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5332; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32753553
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.16.994236v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_149663 Copy
Species: Homo sapiens
Genetic Insert: Angiotensin-converting enzyme 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5332; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32753553
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.16.994236v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_149665 Copy
Species: Homo sapiens
Genetic Insert: Angiotensin-converting enzyme 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5332; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32753553
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.16.994236v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_149668 Copy
Species: Homo sapiens
Genetic Insert: ACE2
Vector Backbone Description: Vector Backbone:pENTR223; Vector Types:Gateway Cloning; Bacterial Resistance:Streptomycin
Comments: BC039902
Proper citation: RRID:Addgene_149719 Copy
Vector Backbone Description: Backbone Size:4492; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1995. Fire Lab Miniprep Number pPD95.81, Ligation number NONE.
Proper citation: RRID:Addgene_1497 Copy
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