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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
CS2 Flag-Smad3 (EPSM)
 
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1+ mentions
RRID:Addgene_14963 Smad3 (EPSM) Homo sapiens Ampicillin PMID:10197981 3SP and 1TP sites in the linker are mutated to alanine or valine (T-DV). See author's map for more information. Please note that the mutations correspond to residues 179, 204, 208, and 213 of the native Smad3 protein. Backbone Size:4100; Vector Backbone:pCS2; Vector Types:xenopus/mammalian/avian/zebrafish; Bacterial Resistance:Ampicillin T178V, S203A, S207A, S212A 2026-08-15 01:07:18 6
pYPQ166-iSpyMac
 
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RRID:Addgene_149553 iSpyMacCas9 Spectinomycin PMID:33898973 Vector Backbone:pYPQ165; Vector Types:Plant Expression, CRISPR, Gateway compatible ziSpyMacCas9 entry clone; Bacterial Resistance:Spectinomycin R221K, N394K, Protospacer interacting domain form Streptococcus macacae 2026-08-15 01:07:17 1
pYPQ265H
 
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RRID:Addgene_149554 hAID-ziSpyMacCas9(D10A)-UGI Spectinomycin PMID:33898973 Vector Backbone:pNJB91; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin D10A, R221K, N394K, Protospacer interacting domain form Streptococcus macacae 2026-08-15 01:07:16 2
pYPQ266B
 
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RRID:Addgene_149555 ziSpyMacCas9(D10A)-PmCDA1-UGI Spectinomycin PMID:33898973 Vector Backbone:pNJB91; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin D10A, R221K, N394K, Protospacer interacting domain form Streptococcus macacae 2026-08-15 01:07:16 2
pCMV5 Smad1-HA
 
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1+ mentions
RRID:Addgene_14956 Smad1 Homo sapiens Ampicillin PMID:8637600 See author's map for more information. Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:07:16 1
pcDNA Flag-Smad4M
 
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1+ mentions
RRID:Addgene_14959 Smad4 Homo sapiens Ampicillin PMID:16172383 Site directed mutagenesis in the coding sequence of Tyr162 (from TAT to TAC) and Val163 (from GTG to GTT). The mutated sequence encodes the same amino acids. See author's map for more information. Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:07:16 2
pBTG (pBigT-IRES-GFP; DM#268)
 
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RRID:Addgene_15037 Ampicillin PMID:14657333 Modification of plasmid pBigT described in Srinivas et al. BMC Dev Biol (2001) vol 1, p.4. For use with pRosa26PA. See BMC Dev Biol article for more information. Modified to contain IRES-EGFP downstream of cloning site. See Author's Map for more information. Backbone Size:7562; Vector Backbone:pBigT; Vector Types:Cre/Lox, for making transgenic constructs; Bacterial Resistance:Ampicillin 2026-08-15 01:07:18 7
pSP64T (DM#111)
 
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RRID:Addgene_15030 beta-globin 5' and 3' UTR Xenopus laevis Ampicillin PMID:6207484 Vector with Xenopus 5' and 3' untranslated beta-globin sequences to enhance translation of synthetic mRNAs. Cloning site for mRNA is BglII. See author's map for more information. Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pSP64; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:07:18 2
pBABEpuro HA Brca1
 
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10+ mentions
RRID:Addgene_14999 Brca1 Homo sapiens Ampicillin PMID:10550055 pBABEpuro HABrca1 was made by digesting pCDNA3beta HABrca1 (R. Scully, et al., Cell 88, 265, 1997) with SalI and XhoI and inserting it into the SalI site of pBABEpuro. The BRCA1 insert in this plasmid contains the following common polymorphisms when compared to GenBank reference sequence NP_009225.1: P871L, E1038G, K1182R, and S1613G. These SNPs were likely present in the original cDNA isolate. Backbone Size:5200; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-15 01:07:18 14
pIRES2-EGFP Mer
 
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1+ mentions
RRID:Addgene_14998 Mer Mus musculus Kanamycin PMID:15673687 The full-length mouse Mer (994 amino acids) was cloned from a 16-day-old mouse embryo cDNA library. Addgene's quality control found the closest match to the insert sequence is mouse Mer (AA17-994) Sequence ID: NP_032613.1, with mutations I516V and Q860R. The plasmid should function as described in the associated article listed on the plasmid page above. Backbone Marker:BD Biosciences; Backbone Size:5300; Vector Backbone:pIRES2-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:07:19 2
pCMV5 Flag-Smad3 (146-425)
 
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1+ mentions
RRID:Addgene_14966 Smad3 Homo sapiens Ampicillin PMID:12150994 See author's map for more information. Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin aa 146-425 2026-08-15 01:07:18 1
pCMV5 Flag-Smad3 (220-425)
 
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1+ mentions
RRID:Addgene_14967 Smad3 Homo sapiens Ampicillin PMID:12150994 See author's map for more information. Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin aa 220-425 2026-08-15 01:07:19 1
pBV-Luc/Del-6
 
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1+ mentions
RRID:Addgene_14969 c-myc promoter (-109/+334) Homo sapiens Ampicillin PMID:11158583 XhoI to PvuII of c-myc promoter was subcloned into pBV-Luc, a low basal activity luciferase reporter plasmid. Backbone Size:4900; Vector Backbone:pBV-luciferase; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin -109/+334 (relative to the P2 transcription initiation site) of the c-myc promoter. 2026-08-15 01:07:17 1
Hsp68-CreER(TM) (DM#264)
 
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1+ mentions
RRID:Addgene_15028 Cre-ER(TM) Ampicillin The CRE-ER(TM) protein requires tamoxifen (TM) to catalyze LoxP site-mediated excision. See Author's Map for more information. Backbone Size:3000; Vector Backbone:modified pBluescript II KS; Vector Types:Mammalian Expression, Cre/Lox, for making transgenic constructs; Bacterial Resistance:Ampicillin 2026-08-15 01:07:18 1
Pdx1 Cre (DM#258)
 
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1+ mentions
RRID:Addgene_15021 Pdx1 promoter Mus musculus Ampicillin PMID:11973276 To generate the Pdx1-Cre construct, a 5.5 kb SalI-SmaI fragment (blunt-ended) containing the Pdx1 promoter from pKSpdx-1SalI was ligated to the XbaI-digested (blunt-ended) pMC-Cre. The insert was released by SalI and NotI digestion. See Author's Map for more information. Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Mammalian Expression, Cre/Lox, mouse transgenic; Bacterial Resistance:Ampicillin 2026-08-15 01:07:18 1
pcDNA3-sACE2v2.2-8his
 
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1+ mentions
RRID:Addgene_149663 Angiotensin-converting enzyme 2 Homo sapiens Ampicillin PMID:32753553 Please visit https://www.biorxiv.org/content/10.1101/2020.03.16.994236v1 for BioRxiv preprint. Backbone Marker:Invitrogen; Backbone Size:5332; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Extracellular, soluble protease domain (a.a. M1-D615) with mutations T27Y, N330Y, A386L 2026-08-15 01:07:18 1
pcDNA3-sACE2v2.2-sfGFP
 
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1+ mentions
RRID:Addgene_149665 Angiotensin-converting enzyme 2 Homo sapiens Ampicillin PMID:32753553 Please visit https://www.biorxiv.org/content/10.1101/2020.03.16.994236v1 for BioRxiv preprint. Backbone Marker:Invitrogen; Backbone Size:5332; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Extracellular, soluble protease domain (a.a. M1-D615) with mutations T27Y, N330Y, A386L 2026-08-15 01:07:17 1
pcDNA3-sACE2v2.4-IgG1
 
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1+ mentions
RRID:Addgene_149668 Angiotensin-converting enzyme 2 Homo sapiens Ampicillin PMID:32753553 Please visit https://www.biorxiv.org/content/10.1101/2020.03.16.994236v1 for BioRxiv preprint. Backbone Marker:Invitrogen; Backbone Size:5332; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Extracellular, soluble protease domain (a.a. M1-D615) with mutations T27Y, L79T, N330Y 2026-08-15 01:07:17 1
pENTR223 ACE2, open
 
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RRID:Addgene_149719 ACE2 Homo sapiens Streptomycin BC039902 Vector Backbone:pENTR223; Vector Types:Gateway Cloning; Bacterial Resistance:Streptomycin wt, without stop codon 2026-08-15 01:07:17 1
pPD95_81
 
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1+ mentions
RRID:Addgene_1497 Ampicillin See Fire Lab Vector Kit Documentation 1995. Fire Lab Miniprep Number pPD95.81, Ligation number NONE. Backbone Size:4492; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:07:19 4

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