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Species: Homo sapiens
Genetic Insert: ADGRG7
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pFuse-hIgG1-Fc2; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:38608683
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.07.14.500097v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_217738 Copy
Species: Homo sapiens
Genetic Insert: ADGRL1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pFuse-hIgG1-Fc2; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:38608683
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.07.14.500097v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_217739 Copy
Species: Homo sapiens
Genetic Insert: ADGRF3
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pFuse-hIgG1-Fc2; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:38608683
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.07.14.500097v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_217696 Copy
Species: Homo sapiens
Genetic Insert: ADGRE5
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pFuse-hIgG1-Fc2; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:38608683
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.07.14.500097v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_217693 Copy
Species: Homo sapiens
Genetic Insert: ADGRF1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pFuse-hIgG1-Fc2; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:38608683
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.07.14.500097v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_217694 Copy
Species: Homo sapiens
Genetic Insert: ADGRG1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pFuse-hIgG1-Fc2; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:38608683
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.07.14.500097v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_217699 Copy
Species: Homo sapiens
Genetic Insert: ADGRG2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pFuse-hIgG1-Fc2; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:38608683
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.07.14.500097v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_217733 Copy
Species: Homo sapiens
Genetic Insert: ADGRF4
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pFuse-hIgG1-Fc2; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:38608683
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.07.14.500097v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_217730 Copy
Species: Homo sapiens
Genetic Insert: ADGRF4
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pFuse-hIgG1-Fc2; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:38608683
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.07.14.500097v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_217697 Copy
Species: Homo sapiens
Genetic Insert: ADGRF5
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pFuse-hIgG1-Fc2; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:38608683
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.07.14.500097v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_217731 Copy
Species: Homo sapiens
Genetic Insert: ADGRL3
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pFuse-hIgG1-Fc2; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:38608683
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.07.14.500097v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_217741 Copy
Species: Homo sapiens
Genetic Insert: ADGRL4
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pFuse-hIgG1-Fc2; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:38608683
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.07.14.500097v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_217742 Copy
Species: Homo sapiens
Genetic Insert: IL2-ss
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:3380; Vector Backbone:pFUSE; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Comments: The VHH section (termed B12) was designed through phage display by the company Hybrigenics Services (Évry-Courcouronnes, France). The nanobody was seen to be specific towards FGFR3 (mouse) and not FGFR1 or FGFR2.
Proper citation: RRID:Addgene_209837 Copy
Species: Homo sapiens
Genetic Insert: IL2-ss
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:3380; Vector Backbone:pFUSE; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Comments: The VHH (termed G08) was designed by Hybrigenics Services (Évry-Courcouronnes, France) by phage display. The VHH insert was then placed into this plasmid. In testing, this nanobody was specific for FGFR3, not FGFR2 and FGFR1.
Proper citation: RRID:Addgene_209857 Copy
Species: Hydrangea macrophylla
Genetic Insert: HmS
Vector Backbone Description: Backbone Size:3382; Vector Backbone:MoClo adapted pGAPZ-vector; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:38457503
Proper citation: RRID:Addgene_219766 Copy
Genetic Insert: zeocin resistance gene
Vector Backbone Description: Backbone Marker:K. Murphy; Backbone Size:816; Vector Backbone:pKM461; Vector Types:Bacterial Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:30538179
Comments: This plasmid can be used to removed ORBIT integrated plasmids leaving behind an Bxb1 attP site. After deletion of a target gene using ORBIT, the integrated plasmid (e.g., pKM464 - hygR) is excised by expressing phage Bxb1 Integrase and gp47 (directionality factor) from pKM512. A strain containing an ORBIT-generated deletion is transformed with pKM512 selecting for zeocin resistance. (This step cures the cell of pKM461 (kanR) used to make the deletion.) Select a zeoR colony containing pKM512 and grow to saturation in 7H10-zeocin. Dilute the culture 100-fold and regrow the strain in 7H10 without zeocin, but include 500 ng/ml anhydrotetracycline, (which induces expression of Integrase and gp47). Grow to saturation again, plate for single colonies, stab colonies on 7H10 plates containing 10% sucrose (Smeg) or 3% sucrose (Mtb) looking for hygromycin-sensitive colonies. Colonies should also be sensitive to kanamycin and zeocin. Once performed, the strain cannot be used for deletion of a second gene using ORBIT, as an attP site is now present at the site of the initial deletion.
Proper citation: RRID:Addgene_140192 Copy
Species: Mus musculus
Genetic Insert: ORF1-2 derived from mouse LINE1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pZeoSV2(+); Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:33938150
Proper citation: RRID:Addgene_206024 Copy
Genetic Insert: hSpCas9
Vector Backbone Description: Backbone Marker:adapted from addgene #75161; Backbone Size:6800; Vector Backbone:lentiCRISPRv2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Bleocin (Zeocin)
Proper citation: RRID:Addgene_199342 Copy
Genetic Insert: hSpCas9
Vector Backbone Description: Backbone Marker:adapted from addgene #99154; Backbone Size:6800; Vector Backbone:lentiCRISPRv2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Bleocin (Zeocin)
Proper citation: RRID:Addgene_199343 Copy
Genetic Insert: hSpCas9
Vector Backbone Description: Backbone Marker:adapted from addgene #99154; Backbone Size:6800; Vector Backbone:lentiCRISPRv2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Bleocin (Zeocin)
Proper citation: RRID:Addgene_199700 Copy
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