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Species: Gallus gallus
Genetic Insert: Fusion Glycoprotein type E
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pCAGGS vector; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24613797
Proper citation: RRID:Addgene_67509 Copy
Species: Gallus gallus
Genetic Insert: Fusion Glycoprotein type B2
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pCAGGS vector; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22114284
Proper citation: RRID:Addgene_67513 Copy
Species: Gallus gallus
Genetic Insert: Fusion Glycoprotein type B
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pCAGGS vector; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20954846
Proper citation: RRID:Addgene_67523 Copy
Species: Gallus gallus
Genetic Insert: actin prom-dsRed-scramble shRNA
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pUC; Vector Types:Mammalian Expression, AAV, RNAi; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25706476
Comments: To express shRNAs, we used the pPRIME system. This generates
micro-RNA-30 (mir30)-derived shRNAs and co-expression of a marker protein, such as dsRED, from the same transcript. The shRNA hairpin sequences were placed into the mir30 site of pPRIME-cmv-dsRed-FF3 and then cloned into the AAV vector (Zhang et al 2015 Nat Neurosci),
SbfI(or PstI) + PacI will retrieve the 2.1kb insert (dsRed-scramble shRNA-chloramphenicol) and the 5.1kb AAV-CBA backbone, which is ampicillin resistant. The SbfI site in the middle of dsRed has been silenced. The construct can be linearised with XhoI.
Proper citation: RRID:Addgene_67879 Copy
Species: Gallus gallus
Genetic Insert: dsRed and shRNA to knock down the mouse adra2 receptor
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pUC; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25706476
Comments: The shRNA hairpin sequences were placed into the mir30 site of pPRIME-cmv-dsRed-FF3 (Addgene Plasmid 11664; gift from S. Elledge, Howard Hughes Medical Institute). Inserts in pPRIME were released by SbfI and PacI digestion, and cloned into the PstI and PacI sites in the polylinker of the AAV-genome plasmid pAM-flex (Murray AJ et al.2011 Nat Neurosci 14, p297), to give ITR-cmv-Penhancer/chicken β-actin-dsRED-mir30-shRNA-woodchuck post-translational regulatory sequence (WPRE)-bovine growth hormone polyadenylation signal (pA)-ITR. The sh RNA knocks down mouse adra2a expression.
Proper citation: RRID:Addgene_67880 Copy
Species: Gallus gallus
Genetic Insert: Fzd7
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCAG; Vector Types:Chicken expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25617437
Proper citation: RRID:Addgene_66106 Copy
Species: Gallus gallus
Genetic Insert: v-Src
Vector Backbone Description: Backbone Marker:BD Biosciences; Backbone Size:6600; Vector Backbone:pLNCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_14578 Copy
Species: Gallus gallus
Genetic Insert: TVA:mCherry
Vector Backbone Description: Backbone Size:5383; Vector Backbone:pAAV-nef-CIAO; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31843925
Comments: Not used in the original publication. Full name: pAAV-nef-AO-lox66-STOP-lox71-TVA:mCherry-hGH-pA.
Proper citation: RRID:Addgene_149295 Copy
Species: Gallus gallus
Genetic Insert: TVA:mCherry
Vector Backbone Description: Backbone Size:5383; Vector Backbone:pAAV-nef-CIAO2; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31843925
Comments: The CIAO2 construct replaces the lox66/71 pair used in CIAO with the lox43/44 pair to further decrease leak expression - not used in the original publication. Full name: pAAV-nef-AO-lox43-STOP-lox44-TVA:mCherry-hGH-pA.
Proper citation: RRID:Addgene_149297 Copy
Species: Gallus gallus
Genetic Insert: CrkII
Vector Backbone Description: Backbone Size:5300; Vector Backbone:pEBB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11314030
Proper citation: RRID:Addgene_15000 Copy
Species: Gallus gallus
Genetic Insert: Cyp26
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP (B); Vector Types:Retroviral, Avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16251210
Proper citation: RRID:Addgene_15154 Copy
Species: Gallus gallus
Genetic Insert: CHX10 3'UTR
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10640715
Comments: The Chx10 cDNA fragment containing 0.95 kb of the 3' untranslated region (UTR) (StuI to 3' end). Used for generating antisense riboprobes.
Proper citation: RRID:Addgene_15150 Copy
Species: Gallus gallus
Genetic Insert: cVax
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCAS; Vector Types:Retroviral, Avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10595508
Proper citation: RRID:Addgene_15136 Copy
Species: Gallus gallus
Genetic Insert: Rax
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP (A); Vector Types:Retroviral, Avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12403708
Proper citation: RRID:Addgene_15194 Copy
Species: Gallus gallus
Genetic Insert: Pdx1
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11230152
Comments: Mutated Pdx-1 protein is unable to bind DNA; made by changing the leucine between helices 2 and 3 of the homeodomain into a proline.
Proper citation: RRID:Addgene_15072 Copy
Species: Gallus gallus
Genetic Insert: RaxL
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP (A); Vector Types:Retroviral, Avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12403708
Proper citation: RRID:Addgene_15193 Copy
Species: Gallus gallus
Genetic Insert: RaxL
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP (B); Vector Types:Retroviral, Avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12403708
Comments: A fusion construct containing the engrailed repressor domain and the homeodomain of RaxL.
Proper citation: RRID:Addgene_15197 Copy
Species: Gallus gallus
Genetic Insert: paxillin
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6100; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16254239
Proper citation: RRID:Addgene_15211 Copy
Species: Gallus gallus
Genetic Insert: paxillin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11425873
Proper citation: RRID:Addgene_15233 Copy
Species: Gallus gallus
Genetic Insert: Fz 1
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(B); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10862751
Comments: Frizzled 1 wild type ORF: 5’ NcoI-primer, 3’ EcoRI-primer,
shuttle vector: Slax13.
There is a L149V mutation in the gene. There is no data regarding how this mutation affects protein function.
Proper citation: RRID:Addgene_13932 Copy
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