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Species: Saccharomyces cerevisiae
Genetic Insert: crCAN1-4.crHIS4-4.crPDR12-3.crADE2-3.S
Vector Backbone Description: Backbone Size:4995; Vector Backbone:pUD628; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29106617
Proper citation: RRID:Addgene_103024 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: crHIS4-4.S
Vector Backbone Description: Backbone Marker:Świat M... Daran-Lapujade PAS FnCpf1, a novel and efficient genome editing tool for Saccharomyces cerevisiae. NAR; Backbone Size:4995; Vector Backbone:pUD628; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29106617
Proper citation: RRID:Addgene_103021 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: crADE2-3.crHIS4-4.S
Vector Backbone Description: Backbone Marker:Świat M... Daran-Lapujade PAS FnCpf1, a novel and efficient genome editing tool for Saccharomyces cerevisiae. NAR; Backbone Size:4995; Vector Backbone:pUD628; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29106617
Proper citation: RRID:Addgene_103020 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Csy4
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:5522; Vector Backbone:p413 TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29161506
Proper citation: RRID:Addgene_103139 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: dCas9-VPR
Vector Backbone Description: Vector Backbone:pDTU-113; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29161506
Proper citation: RRID:Addgene_103140 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNAs
Vector Backbone Description: Vector Backbone:dCas9v2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29161506
Proper citation: RRID:Addgene_103141 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: S. cerevisiae TEF2 promoter
Vector Backbone Description: Backbone Size:4894; Vector Backbone:pFA6a–link–yEGFP–Kan; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33311538
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.05.11.089300v1 for bioRxiv preprint.
Parent vector is described in "Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae", Mark A Sheff , Kurt S ThornYeast 2004 Jun;21(8):661-70. doi: 10.1002/yea.1130. The parent vector was intended for GFP tagging of yeast proteins. We placed the TEF2 promoter directly upstream of GFP for sugar-insensitive expression of GFP under galactose or dextrose conditions.
Proper citation: RRID:Addgene_160432 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CUP2
Vector Backbone Description: Backbone Marker:self-made; derived from the BioBrick assembly plasmid pSB1C3 ; Vector Backbone:pUPD2; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: Compatible with GoldenBraid; insert can be released with BsaI
Proper citation: RRID:Addgene_160599 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RNA Polymerase II CTD
Vector Backbone Description: Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9468530
Proper citation: RRID:Addgene_160688 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CIM5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16810255
Proper citation: RRID:Addgene_16074 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ull1 delta440
Vector Backbone Description: Backbone Size:5443; Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12761287
Proper citation: RRID:Addgene_16087 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SMT3
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGEX-KG; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11577116
Proper citation: RRID:Addgene_16092 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: UBC9
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGEX-KG; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12761287
Proper citation: RRID:Addgene_16090 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VRG4
Vector Backbone Description: Backbone Size:4000; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30858194
Comments: Parental strain JK93da.
Proper citation: RRID:Addgene_160904 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Medium-chain fatty acid ethyl ester synthase
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32886882
Proper citation: RRID:Addgene_160864 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MCP-mCherry-HIS tag, 3'UTR MS2 Stem Loop
Vector Backbone Description: Backbone Size:6000; Vector Backbone:plIVMD156; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33037152
Proper citation: RRID:Addgene_160935 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MCP-sfGFP-HIS tag, 3'UTR MS2 Stem Loop
Vector Backbone Description: Backbone Size:6000; Vector Backbone:plIVMD156; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33037152
Comments: Please note that the T247A mutation in sfGFP does not impact plasmid function.
Proper citation: RRID:Addgene_160936 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MCP-mTagBFP2-HA Tag
Vector Backbone Description: Vector Backbone:psh100; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33037152
Proper citation: RRID:Addgene_160939 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HHF1
Vector Backbone Description: Backbone Marker:Berkeley Macrolab; Backbone Size:4786; Vector Backbone:pET (https://www.addgene.org/29666/); Vector Types:; Bacterial Resistance:Ampicillin
Comments: This is an improved version of the plasmid reported here:
https://www.sciencedirect.com/science/article/abs/pii/S096921261930440X
https://doi.org/10.1016/j.str.2019.12.002
All histone genes code for S. cerevisiae proteins. Expression and purification procedures are as described.
Proper citation: RRID:Addgene_160930 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HHF1
Vector Backbone Description: Backbone Marker:Berkeley Macrolab; Backbone Size:4786; Vector Backbone:pET (https://www.addgene.org/29666/); Vector Types:; Bacterial Resistance:Ampicillin
Comments: This is an improved version of the plasmid reported here:
https://www.sciencedirect.com/science/article/abs/pii/S096921261930440X
https://doi.org/10.1016/j.str.2019.12.002
All histone genes code for S. cerevisiae proteins.
Proper citation: RRID:Addgene_160929 Copy
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