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Species: M. smegmatis
Genetic Insert: mceG
Vector Backbone Description: Vector Backbone:pMV261-Zeo; Vector Types:Bacterial Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:36711512
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.12.08.519548v2.full for bioRxiv preprint.
Proper citation: RRID:Addgene_201996 Copy
Species: M. smegmatis
Genetic Insert: mceG
Vector Backbone Description: Vector Backbone:pMV261-Zeo; Vector Types:Bacterial Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:36711512
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.12.08.519548v2.full for bioRxiv preprint.
Proper citation: RRID:Addgene_201990 Copy
Species: M. smegmatis
Genetic Insert: mceG
Vector Backbone Description: Vector Backbone:pMV261-Zeo; Vector Types:Bacterial Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:36711512
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.12.08.519548v2.full for bioRxiv preprint.
Proper citation: RRID:Addgene_201972 Copy
Species: M. smegmatis
Genetic Insert: mceG
Vector Backbone Description: Vector Backbone:pMV261-Zeo; Vector Types:Bacterial Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:36711512
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.12.08.519548v2.full for bioRxiv preprint.
Proper citation: RRID:Addgene_201978 Copy
Species: Mus musculus
Genetic Insert: PLD4
Vector Backbone Description: Backbone Size:2000; Vector Backbone:SuExp; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:34620855
Proper citation: RRID:Addgene_201185 Copy
Genetic Insert: zeocin resistance gene
Vector Backbone Description: Backbone Marker:K. Murphy; Backbone Size:816; Vector Backbone:pKM461; Vector Types:Bacterial Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:30538179
Comments: This plasmid can be used to removed ORBIT integrated plasmids leaving behind an Bxb1 attP site. After deletion of a target gene using ORBIT, the integrated plasmid (e.g., pKM464 - hygR) is excised by expressing phage Bxb1 Integrase and gp47 (directionality factor) from pKM512. A strain containing an ORBIT-generated deletion is transformed with pKM512 selecting for zeocin resistance. (This step cures the cell of pKM461 (kanR) used to make the deletion.) Select a zeoR colony containing pKM512 and grow to saturation in 7H10-zeocin. Dilute the culture 100-fold and regrow the strain in 7H10 without zeocin, but include 500 ng/ml anhydrotetracycline, (which induces expression of Integrase and gp47). Grow to saturation again, plate for single colonies, stab colonies on 7H10 plates containing 10% sucrose (Smeg) or 3% sucrose (Mtb) looking for hygromycin-sensitive colonies. Colonies should also be sensitive to kanamycin and zeocin. Once performed, the strain cannot be used for deletion of a second gene using ORBIT, as an attP site is now present at the site of the initial deletion.
Proper citation: RRID:Addgene_140192 Copy
Species: Homo sapiens
Genetic Insert: DJ1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pcDNA3.1/GS; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Proper citation: RRID:Addgene_29411 Copy
Species: Homo sapiens
Genetic Insert: DJ1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pcDNA3.1/GS; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Proper citation: RRID:Addgene_29410 Copy
Species: Homo sapiens
Genetic Insert: DJ1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pcDNA3.1/GS; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:12851414
Proper citation: RRID:Addgene_29346 Copy
Species: Homo sapiens
Genetic Insert: DJ1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pcDNA3.1/GS; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:12851414
Proper citation: RRID:Addgene_29344 Copy
Species: Homo sapiens
Genetic Insert: DJ1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pcDNA3.1/GS; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:12851414
Proper citation: RRID:Addgene_29343 Copy
Species: Homo sapiens
Genetic Insert: DJ1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pcDNA3.1/GS; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:12851414
Proper citation: RRID:Addgene_29342 Copy
Species: Homo sapiens
Genetic Insert: DJ1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pcDNA3.1/GS; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:12851414
Proper citation: RRID:Addgene_29341 Copy
Species: Homo sapiens
Genetic Insert: p21
Vector Backbone Description: Backbone Marker:Invivogen; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:23299246
Proper citation: RRID:Addgene_40206 Copy
Species: Homo sapiens
Genetic Insert: p21
Vector Backbone Description: Backbone Marker:Invivogen; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:23299246
Proper citation: RRID:Addgene_40204 Copy
Species: Homo sapiens
Genetic Insert: p21
Vector Backbone Description: Backbone Marker:Invivogen; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:23299246
Proper citation: RRID:Addgene_40203 Copy
Species: Homo sapiens
Genetic Insert: p21
Vector Backbone Description: Backbone Marker:Invivogen; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:23299246
Proper citation: RRID:Addgene_40202 Copy
Species: virus
Genetic Insert: MCV Large T antigen
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:3429; Vector Backbone:pMONO-zeo; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:21829355
Comments: Please consult laboratory website, http://home.ccr.cancer.gov/Lco/plasmids.asp, for more information.
Proper citation: RRID:Addgene_32097 Copy
Species: virus
Genetic Insert: BKV VP1
Vector Backbone Description: Backbone Size:5338; Vector Backbone:pGwf; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:21829355
Comments: Please consult laboratory website, http://home.ccr.cancer.gov/Lco/plasmids.asp, for more information.
Proper citation: RRID:Addgene_32094 Copy
Species: virus
Genetic Insert: BKV VP2
Vector Backbone Description: Backbone Size:4508; Vector Backbone:phGf; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:21829355
Comments: Please consult laboratory website, http://home.ccr.cancer.gov/Lco/plasmids.asp, for more information.
Proper citation: RRID:Addgene_32109 Copy
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