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Species: Streptococcus pyogenes
Genetic Insert: PcUB4-2::spCas9
Vector Backbone Description: Vector Backbone:pDe-CAS9; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:34717688
Comments: in addition to Cas9 cassette, attR1-attR2 Gateway recombination sites together with ccdB cassette are present.
Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167677 Copy
Vector Backbone Description: Backbone Marker:Invitrogen modified by doi:10.1271/bbb.70678; Backbone Size:10835; Vector Backbone:R4pGWB401; Vector Types:Plant Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:31422517
Comments: For use with Three-way Multi-site Gateway Cloning (Invitrogen).
Proper citation: RRID:Addgene_128432 Copy
Vector Backbone Description: Backbone Size:9189; Vector Backbone:pTF101.1; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Comments: Contains the 564bp bialaphos resistance (bar) gene from Streptomyces hygroscopicus
Proper citation: RRID:Addgene_134768 Copy
Vector Backbone Description: Vector Backbone:pP7m34GW; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:32601420
Proper citation: RRID:Addgene_133747 Copy
Vector Backbone Description: Vector Backbone:pBiFCt-2in1-CC; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:23066669
Proper citation: RRID:Addgene_105114 Copy
Vector Backbone Description: Vector Backbone:pFRETgc-2in1-CC; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:25971551
Proper citation: RRID:Addgene_105115 Copy
Vector Backbone Description: Vector Backbone:pBiFCt-2in1-NC; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:23066669
Proper citation: RRID:Addgene_105112 Copy
Vector Backbone Description: Vector Backbone:pFRETtv-2in1-CC; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:25971551
Proper citation: RRID:Addgene_105116 Copy
Vector Backbone Description: Vector Backbone:pFRETgc-2in1-NC; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:25971551
Proper citation: RRID:Addgene_105121 Copy
Vector Backbone Description: Vector Backbone:pFRETvr-2in1-CN; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:25971551
Proper citation: RRID:Addgene_105120 Copy
Vector Backbone Description: Vector Backbone:pUBC-paGFP-Dest; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:24057383
Proper citation: RRID:Addgene_105107 Copy
Vector Backbone Description: Vector Backbone:pAGM1287; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:29847550
Proper citation: RRID:Addgene_105339 Copy
Species: Synthetic
Genetic Insert: pIM13 ORI plus NGS barcode 28, spectinomycin resistance cassette, mScarlet-I cassette, oriT
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Spectinomycin
Comments: Please note that this plasmid may run as a dimer or higher multimer.
Proper citation: RRID:Addgene_251929 Copy
Species: Synthetic
Genetic Insert: pBP1 ORI plus NGS barcode 11, spectinomycin resistance cassette, mScarlet-I cassette, oriT
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Spectinomycin
Comments: Please note that this plasmid may run as a dimer or higher multimer.
Proper citation: RRID:Addgene_251925 Copy
Species: Synthetic
Genetic Insert: pBC1 ORI plus NGS barcode 25, spectinomycin resistance cassette, mScarlet-I cassette, oriT
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Spectinomycin
Comments: Please note that this plasmid may run as a dimer or higher multimer.
Proper citation: RRID:Addgene_251924 Copy
Species: Synthetic
Genetic Insert: pBBR1-UP ORI plus NGS barcode 21, spectinomycin resistance cassette, mScarlet-I cassette, oriT
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Spectinomycin
Comments: Please note that this plasmid may run as a dimer or higher multimer.
Proper citation: RRID:Addgene_251923 Copy
Species: Synthetic
Genetic Insert: pBBR1 ORI plus NGS barcode 13, spectinomycin resistance cassette, mScarlet-I cassette, oriT
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Spectinomycin
Comments: Please note that this plasmid may run as a dimer or higher multimer.
Proper citation: RRID:Addgene_251922 Copy
Species: Synthetic
Genetic Insert: pAL5000 ORI plus NGS barcode 1, spectinomycin resistance cassette, mScarlet-I cassette, oriT
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Spectinomycin
Comments: Please note that this plasmid may run as a dimer or higher multimer.
Proper citation: RRID:Addgene_251920 Copy
Species: Synthetic
Genetic Insert: pUB110 ORI plus NGS barcode 2, spectinomycin resistance cassette, mScarlet-I cassette, oriT
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Spectinomycin
Comments: Please note that this plasmid may run as a dimer or higher multimer.
Proper citation: RRID:Addgene_251937 Copy
Species: Synthetic
Genetic Insert: pTHT15 ORI plus NGS barcode 30, spectinomycin resistance cassette, mScarlet-I cassette, oriT
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Spectinomycin
Comments: Please note that this plasmid may run as a dimer or higher multimer.
Proper citation: RRID:Addgene_251936 Copy
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