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Species: Pseudomonas aeruginosa UCBPP-PA14
Genetic Insert: Pseudomonas aeruginosa PA14 CRISPR2 locus
Vector Backbone Description: Backbone Size:5220; Vector Backbone:pHERD30T; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:34174210
Proper citation: RRID:Addgene_149396 Copy
Species: Pseudomonas aeruginosa UCBPP-PA14
Genetic Insert: Pseudomonas aeruginosa PA14 CRISPR2 locus
Vector Backbone Description: Backbone Size:5220; Vector Backbone:pHERD30T; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:34174210
Proper citation: RRID:Addgene_149397 Copy
Species: C. crescentus
Genetic Insert: mScarletI
Vector Backbone Description: Vector Backbone:pMT335; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:36076039
Proper citation: RRID:Addgene_174506 Copy
Species: Homo sapiens
Genetic Insert: KIF5A (∆exon27)
Vector Backbone Description: Backbone Marker:GENEVA Biotech; Backbone Size:3641; Vector Backbone:pACEBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:35430760
Proper citation: RRID:Addgene_184827 Copy
Vector Backbone Description: Backbone Size:10827; Vector Backbone:pJC005.gent; Vector Types:CRISPR; Bacterial Resistance:Gentamicin
Defining Citation: PMID:35107356
Proper citation: RRID:Addgene_182739 Copy
Genetic Insert: MKK7-EE
Vector Backbone Description: Backbone Marker:Addgene #83274; Backbone Size:4496; Vector Backbone:pEN_TTmiRc2 3xFLAG; Vector Types:; Bacterial Resistance:Gentamicin
Defining Citation: PMID:28174228
Proper citation: RRID:Addgene_87777 Copy
Species: Homo sapiens
Genetic Insert: MKK4
Vector Backbone Description: Backbone Marker:Addgene #83274; Backbone Size:4496; Vector Backbone:pEN_TTmiRc2 3xFLAG; Vector Types:; Bacterial Resistance:Gentamicin
Defining Citation: PMID:28174228
Proper citation: RRID:Addgene_87776 Copy
Genetic Insert: xylE
Vector Backbone Description: Backbone Marker:PMID: 9380741; Backbone Size:8550; Vector Backbone:pPR23; Vector Types:Allelic change mutagenesis; Bacterial Resistance:Gentamicin
Defining Citation: PMID:17074913
Comments: SacB is used for counter-selecton in Mycobacteria to force excision and loss of this plasmid to occur.
This plasmid has been used as an allelic exchange vector. We have used SpeI site for cloning of an insert (flanking regions of a target gene to be deleted), so other groups can also use SpeI (NotI and XbaI can be used as well).
Proper citation: RRID:Addgene_89632 Copy
Species: Synthetic
Genetic Insert: sfGFP pOGG037, T-pharma pOGG003 assembled in pOGG024
Vector Backbone Description: Vector Backbone:pOGG024; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:30692983
Comments: Please visit https://www.biorxiv.org/content/early/2018/08/16/392423 for bioRxiv preprint.
Proper citation: RRID:Addgene_115508 Copy
Vector Backbone Description: Backbone Size:57000; Vector Backbone:pTA-Mob 2.0; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37850145
Proper citation: RRID:Addgene_188616 Copy
Vector Backbone Description: Backbone Marker:Strand et al., 2014; Backbone Size:56570; Vector Backbone:pTA-Mob; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology, Conjugation helper plasmid; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37850145
Proper citation: RRID:Addgene_188619 Copy
Species: Other
Genetic Insert: PE2 (synthetic), hU6 HEK3 PegRNA, hU6 HEK3 sgRNA, aeBlue (E. quadricolor), VSV-G, TagBFP
Vector Backbone Description: Vector Backbone:pMMACE DEST CMV PE2; Vector Types:Mammalian Expression, CRISPR, Recombinant baculovirus production, CRE-acceptor; Bacterial Resistance:Gentamicin
Defining Citation: PMID:35801912
Proper citation: RRID:Addgene_206274 Copy
Genetic Insert: dddI
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:32641830
Comments: Genotype: F– φ80lacZΔM15 Δ(lacZYAargF)U169 recA1 endA1 hsdR17(rK–, mK+) phoA supE44 λ– thi-1 gyrA96 relA1, with chromosomally integrated dddI
PCR verification primers (expected product: ~450 bp): dddI-F: 5'-ATTCGAGCTCACGGGAGGAA-3' and dddI-R: 5'-CCTGCAGGTCGACCTCATTT-3'
Proper citation: RRID:Addgene_179631 Copy
Species: Synthetic
Genetic Insert: gRNA scaffold - Gm resistance cassette - incP oriT
Vector Backbone Description: Backbone Size:8398; Vector Backbone:pS448∙CsR; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37798358
Proper citation: RRID:Addgene_197860 Copy
Species: Synthetic
Genetic Insert: GAL4BD-VP16
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDON207; Vector Types:Plant Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37323061
Comments: Encodes a chimeric transcription factor ORF consisting of the yeast GAL4 DNA binding domain and HSV VP16 activation domain. No promoter.
Proper citation: RRID:Addgene_201230 Copy
Species: Synthetic
Genetic Insert: mScarlet counter-selection cassette
Vector Backbone Description: Backbone Size:1892; Vector Backbone:BASIC_SEVA_66.11; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
Defining Citation: PMID:36381610
Proper citation: RRID:Addgene_194213 Copy
Species: Synthetic
Genetic Insert: CVB3 2A protease with HisTag
Vector Backbone Description: Backbone Marker:Dept of Molec Med and Biotech, A.I. Virtanen Institute, University of Kuopio, PO Box 1627 FIN-70211, Kuopio, Finland; Backbone Size:9972; Vector Backbone:pBVboostFGII WPRE; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37227147
Comments: Insert cloned to plasmid with attL1 and attL2 sites
Proper citation: RRID:Addgene_202076 Copy
Species: Synthetic
Genetic Insert: CVB3 3C protease with HisTag
Vector Backbone Description: Backbone Marker:Dept of Molec Med and Biotech, A.I. Virtanen Institute, University of Kuopio, PO Box 1627 FIN-70211, Kuopio, Finland; Backbone Size:9972; Vector Backbone:pBVboostFGII WPRE; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37227147
Comments: Insert cloned to plasmid with attL1 and attL2 sites
Proper citation: RRID:Addgene_202077 Copy
Genetic Insert: Pseudomonas aeruginosa PA14 CRISPR2 locus
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHERD30T; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:34174210
Proper citation: RRID:Addgene_162318 Copy
Species: Pseudomonas aeruginosa UCBPP-PA14
Genetic Insert: Pseudomonas aeruginosa PA14 CRISPR2 locus
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHERD30T; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:34174210
Proper citation: RRID:Addgene_162319 Copy
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