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Species: Homo sapiens
Genetic Insert: H2Afy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6100; Vector Backbone:pcDNA3.1-CT-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11331621
Proper citation: RRID:Addgene_45169 Copy
Genetic Insert: Hec1-targeted mCherry Plk1 T210D
Vector Backbone Description: Backbone Size:5348; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22908307
Proper citation: RRID:Addgene_45205 Copy
Species: Homo sapiens
Genetic Insert: Drp1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9786947
Comments: The role of dynamin-related protein 1, a mediator of mitochondrial fission, in apoptosis.
Frank S, Gaume B, Bergmann-Leitner ES, Leitner WW, Robert EG, Catez F, Smith CL, Youle RJ
In Dev Cell. 2001 Oct;1(4):515-25.
Proper citation: RRID:Addgene_45161 Copy
Species: Homo sapiens
Genetic Insert: TNIK
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pBabe-puro-flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23764425
Comments: The TNIK open reading frame was PCR amplified with BP flanked primers from the vector acquired from Open Biosystems and ligated into a pDONR223 vector in a BP Gateway ligation reaction. The TNIK open reading frame was subsequently ligated into the pBabe_puro_DEST_flag backbone in a Gateway LR ligation reaction.
Proper citation: RRID:Addgene_45276 Copy
Species: Homo sapiens
Genetic Insert: LAMP1-mCherry
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20228815
Proper citation: RRID:Addgene_45147 Copy
Species: Homo sapiens
Genetic Insert: SLC7A14
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pBabe-puro-flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23764425
Proper citation: RRID:Addgene_45261 Copy
Species: Synthetic
Genetic Insert: mexF, PP_3426
Vector Backbone Description: Vector Backbone:pBbA5k; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21556065
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_45405 Copy
Species: Chlorocebus aethiops (African green monkey)
Genetic Insert: MAVS
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22427742
Proper citation: RRID:Addgene_45368 Copy
Species: Synthetic
Genetic Insert: AmCyan
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4729; Vector Backbone:pmR-mCherry; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27656125
Comments: the nAmCyan and the mCherry genes are separated by a P2A sequence that results in 2 separate proteins. It also includes BsmBI restriction sites flanking the nAmCyan gene which result in BsiWI and BssHII overhangs for cloning in-frame with P2A-mCherry.
Proper citation: RRID:Addgene_45350 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5488; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_45346 Copy
Vector Backbone Description: Vector Backbone:pGRB2.0; Vector Types:C. glabrata; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23934995
Proper citation: RRID:Addgene_45341 Copy
Species: Synthetic
Genetic Insert: yeGFP
Vector Backbone Description: Vector Backbone:pBM16; Vector Types:C. glabrata expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23934995
Comments: Please note that Addgene's sequencing results found a single nucleotide insertion between bp# 427 and 428 when compared to the full plasmid sequence. According to the depositing laboratory, this insertion has no effect on the function of the plasmid.
Proper citation: RRID:Addgene_45326 Copy
Vector Backbone Description: Backbone Size:6143; Vector Backbone:pGRB2.1; Vector Types:C. glabrata expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23934995
Proper citation: RRID:Addgene_45319 Copy
Species: Escherichia coli
Genetic Insert: rpoA
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3993; Vector Backbone:pGEMEX1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23389035
Proper citation: RRID:Addgene_45398 Copy
Species: Bos taurus
Genetic Insert: Rhodopsin
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22855808
Proper citation: RRID:Addgene_45399 Copy
Species: Macaca mulatta (Rhesus monkey)
Genetic Insert: MAVS
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22427742
Proper citation: RRID:Addgene_45372 Copy
Species: Mus musculus
Genetic Insert: PKA regulatory subunit RI beta tagged by mEGFP
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19447092
Proper citation: RRID:Addgene_45526 Copy
Species: Mus musculus
Genetic Insert: TRPM7
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pcDNA4/TO (modified); Vector Types:Mammalian Expression, Tetracycline Inducible; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11385574
Comments: For the purpose of expressing LTRPC7 in eukaryotic cells, the depositing lab used PCR to produce an epitope tagged expression construct from two overlapping murine LTRPC7 clones. The LTRPC7 coding sequence was modified by removing the initiating methionine and replacing it with a sequence encoding a Kozak sequence, the FLAG tag and the additional sequence GCGGCCGCAT, and by placing a SpeI site just after the stop codon. These modifications result in an expressed protein which started with the following amino acid sequence: MGDYKDDDDKRPH followed by the murine LTRPC7 coding sequence starting at the second amino acid. This construct is expressed from the pcDNA4/TO vector which provides tetracycline-controlled expression from a CMV promotor.
Proper citation: RRID:Addgene_45482 Copy
Species: Rhodopseudomonas palustris
Genetic Insert: iRFP670
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4055; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23770755
Proper citation: RRID:Addgene_45466 Copy
Species: Rhodopseudomonas palustris
Genetic Insert: iRFP713
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4038; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23770755
Comments: For more information on in vivo imaging, please see:
https://www.addgene.org/fluorescent_proteins/in_vivo/
Proper citation: RRID:Addgene_45465 Copy
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