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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pGEX-4T-1 3xHA-MEK1-DD
 
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1+ mentions
RRID:Addgene_47576 MEK1-DD Mus musculus Ampicillin PMID:23233447 Backbone Marker:Amersham; Backbone Size:5062; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Ser218 to Asp, Ser221 to Asp 2026-08-15 01:15:43 1
pGEX-4T-1 3xHA-MKK6-EE
 
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1+ mentions
RRID:Addgene_47579 MKK6-EE Homo sapiens Ampicillin PMID:23233447 Backbone Marker:Amersham; Backbone Size:5062; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Ser207 to Glu, Thr211 to Glu 2026-08-15 01:15:43 1
GST-N1IC
 
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RRID:Addgene_47612 Notch1 intracellular domain Mus musculus Ampicillin PMID:10433920 Construct also called GST-1111. Backbone Marker:Amersham; Backbone Size:4900; Vector Backbone:pGEX-4t-3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin contains amino acids 1753-2531 2026-08-15 01:15:42 1
pGEX-4T-1 3xFlag-JNK1a1
 
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RRID:Addgene_47574 JNK1a1 Homo sapiens Ampicillin PMID:23233447 Backbone Marker:Amersham; Backbone Size:5053; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:15:42 1
EGFPC1-hMyoX
 
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RRID:Addgene_47608 MyoX Homo sapiens Kanamycin PMID:17130134 hMyoX contains a Q680R variant compared to the NCBI reference [AAF37875.1]. The plasmid should function as described in the associated publication. Backbone Marker:Clontech; modified as described in Rogers et al JBC 2001; Backbone Size:4700; Vector Backbone:EGFPC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin See depositor comments below. 2026-08-15 01:15:42 9
EYFP-hCaM
 
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1+ mentions
RRID:Addgene_47603 CaM Homo sapiens Kanamycin PMID:11278607 Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:15:42 5
EGFP-hCaM
 
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1+ mentions
RRID:Addgene_47602 CaM Homo sapiens Kanamycin Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:15:42 5
12XCSL-DsRedExpressDL
 
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1+ mentions
RRID:Addgene_47683 TP-1 promoter Ampicillin PMID:16508309 This plasmid was constructed from the pGa981-6 plasmid by replacing the luciferase gene in the original pGA981-6 plasmid with dsRedExpress (Clontech) using XhoI/SmaI sites. The construction of pGA981-6 was described as: "Six copies of this oligonucleotide were cloned in the BamHI site: 5' strand GATCCCGACT CGTGGGAAAA TGGGCGGAAG GGCACCGTGG GAAAATAGTA............ 3' strand ............GGCTGA GCACCCTTTT ACCCGCCTTC CCGTGGCACC CTTTTATCAT CTAG" Please note that Addgene's sequencing results indicate that the first and fourth ERE-TP1 repeats differ from the above oligo sequence at the twelfth nucleotide (G->A). These differences may have occurred during construction of the original pGA981-6 plasmid. Backbone Marker:described in Kato, et al. 1997 PMID 9374409 ; Vector Backbone:pGA981-6; Vector Types:Notch reporter construct; Bacterial Resistance:Ampicillin hexamerized 50-bp EBNA2 response element of the TP-1 promoter (ERE-TP1) 2026-08-15 01:15:43 6
CFP-Parkin
 
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1+ mentions
RRID:Addgene_47560 Parkin Homo sapiens Kanamycin PMID:19029340 Backbone Marker:Clontech; Backbone Size:4698; Vector Backbone:pECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:15:42 5
pFAB3992
 
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1+ mentions
RRID:Addgene_47823 promoter 14 and BCD16 Synthetic Kanamycin PMID:23474465 http://biofab.org/services RFP fluorescence (Arbitrary Units, AU) is used to measure the efficiency of the promoter/BCD combination. Promoter/BCD combinations ranged from a low score of 6.14 to a high score of 634.54. The score for this combination was: 40.4 Backbone Size:3000; Vector Backbone:pFAB1781; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin 2026-08-15 01:15:45 1
RH2b-bio
 
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1+ mentions
RRID:Addgene_47778 Codon-optimised RH2b (partial) Synthetic Ampicillin PMID:24043421 Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Exogenous signal peptide of mouse origin, changed Serine45, Serine107, Serine125, Threonine161, Serine577, Serine581, Threonine637, Serine677, Serine726, Serine747, Serine783, Serine839 and Serine996 to Alanine residues 2026-08-15 01:15:44 1
pET-PesaRlux
 
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1+ mentions
RRID:Addgene_47802 PesaR-luxCDABE-term Ampicillin PMID:23879176 PesaR-luxCDABE-transcriptional terminator fragment from pCS-PesaRlux is between XhoI and BglII sites in pET17b. PesaR (274bp, indicated as lowercase letters in the 2nd partial sequence for PesaR between XhoI and BamHI) is located between XhoI and BamHI sites. luxCDABE is located between two NotI sites. The transcriptional terminator is located downstream of luxCDABE between the second NotI and BglII. Sequencing primers provided were used to sequence PesaR. Backbone Marker:Novagen; Backbone Size:2934; Vector Backbone:pET-17b; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:15:44 1
pMM2-hPMCA4b
 
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1+ mentions
RRID:Addgene_47759 PMCA4b Homo sapiens Ampicillin PMID:1323273 Note that PMCA4 residue 2 is Ala (instead of Thr) due to an optimized initiation sequence (ATG GCG). Backbone Marker: Adamo, H.P. et al. (1992) Biochem. J. 285, 791-797; Vector Backbone:pMM2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:15:45 1
pSL690
 
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1+ mentions
RRID:Addgene_47753 dCas9-VP64 Synthetic Ampicillin PMID:23892898 Plasmid Features: Cas9 = 409-4512 NLS = 4519-4539 3X FLAG = 4546-4611 VP64 = 4633-4794 Vector Backbone:unknown; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin D10A and H840A mutations in Cas9 2026-08-15 01:15:45 1
pMLM3705
 
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10+ mentions
RRID:Addgene_47754 codon optimized dCas9-VP64 Synthetic Ampicillin PMID:23892898 Plasmid Features: Cas9 = 409-4512 NLS = 4519-4539 3X FLAG = 4546-4611 VP64 = 4633-4794 Backbone Marker:Joung lab (Addgene plasmid # 43861); Vector Backbone:pJDS246; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin D10A and H840A mutations in Cas9 (catalytically inactive) 2026-08-15 01:15:44 18
EBA140-bio
 
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1+ mentions
RRID:Addgene_47742 Codon-optimised EBA140 Synthetic Ampicillin PMID:24043421 Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Exogenous signal peptide of mouse origin, changed Serine78, Threonine92, Serine131, Serine303, Threonine469, Serine727, Serine856, Threonine870, Serine893, Serine1028 and Serine1079 to Alanine residues 2026-08-15 01:15:43 1
AMA1-bio
 
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1+ mentions
RRID:Addgene_47741 Codon-optimised AMA1 Synthetic Ampicillin PMID:24043421 Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Exogenous signal peptide of mouse origin, changed Threonine164, Threonine288, Serine373, Serine423, Serine424 and Threonine501 to Alanine residues 2026-08-15 01:15:45 2
MSP3-bio
 
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1+ mentions
RRID:Addgene_47731 Codon-optimised MSP3 Synthetic Ampicillin PMID:24043421 Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Exogenous signal peptide of mouse origin, changed Serine45, Threonine56, Serine96, Serine226 to Alanine residues 2026-08-15 01:15:43 2
PF3D7_1136200-bio
 
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1+ mentions
RRID:Addgene_47730 Codon-optimised PF3D7_1136200 Synthetic Ampicillin PMID:24043421 Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Exogenous signal peptide of mouse origin replaced endogenous signal peptide. Created the following point mutations: S132A, S138A, S148A, T167A, T184A, S270A, S289A, T322A, T326A, S336A, S337A, S353A, T363A, S557A, T564A, T629A, T633A, T639A, T648A and S649A 2026-08-15 01:15:43 1
Pf113-bio
 
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1+ mentions
RRID:Addgene_47729 Codon-optimised Pf113 Synthetic Ampicillin PMID:24043421 Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Exogenous signal peptide of mouse origin, changed Serine209, Serine270, Threonine319, Serine362, Serine663, Threonine699, Serine781, Threonine878 and Threonine940 to Alanine residues 2026-08-15 01:15:44 2

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