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Species: Mus musculus
Genetic Insert: MEK1-DD
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5062; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23233447
Proper citation: RRID:Addgene_47576 Copy
Species: Homo sapiens
Genetic Insert: MKK6-EE
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5062; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23233447
Proper citation: RRID:Addgene_47579 Copy
Species: Mus musculus
Genetic Insert: Notch1 intracellular domain
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4900; Vector Backbone:pGEX-4t-3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10433920
Comments: Construct also called GST-1111.
Proper citation: RRID:Addgene_47612 Copy
Species: Homo sapiens
Genetic Insert: JNK1a1
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5053; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23233447
Proper citation: RRID:Addgene_47574 Copy
Species: Homo sapiens
Genetic Insert: MyoX
Vector Backbone Description: Backbone Marker:Clontech; modified as described in Rogers et al JBC 2001; Backbone Size:4700; Vector Backbone:EGFPC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17130134
Comments: hMyoX contains a Q680R variant compared to the NCBI reference [AAF37875.1]. The plasmid should function as described in the associated publication.
Proper citation: RRID:Addgene_47608 Copy
Species: Homo sapiens
Genetic Insert: CaM
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11278607
Proper citation: RRID:Addgene_47603 Copy
Species: Homo sapiens
Genetic Insert: CaM
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_47602 Copy
Genetic Insert: TP-1 promoter
Vector Backbone Description: Backbone Marker:described in Kato, et al. 1997 PMID 9374409 ; Vector Backbone:pGA981-6; Vector Types:Notch reporter construct; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16508309
Comments: This plasmid was constructed from the pGa981-6 plasmid by replacing the luciferase gene in the original pGA981-6 plasmid with dsRedExpress (Clontech) using XhoI/SmaI sites.
The construction of pGA981-6 was described as: "Six copies of this oligonucleotide were cloned in the BamHI site:
5' strand GATCCCGACT CGTGGGAAAA TGGGCGGAAG GGCACCGTGG GAAAATAGTA............
3' strand ............GGCTGA GCACCCTTTT ACCCGCCTTC CCGTGGCACC CTTTTATCAT CTAG"
Please note that Addgene's sequencing results indicate that the first and fourth ERE-TP1 repeats differ from the above oligo sequence at the twelfth nucleotide (G->A). These differences may have occurred during construction of the original pGA981-6 plasmid.
Proper citation: RRID:Addgene_47683 Copy
Species: Homo sapiens
Genetic Insert: Parkin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4698; Vector Backbone:pECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19029340
Proper citation: RRID:Addgene_47560 Copy
Species: Synthetic
Genetic Insert: promoter 14 and BCD16
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pFAB1781; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23474465
Comments: http://biofab.org/services
RFP fluorescence (Arbitrary Units, AU) is used to measure the efficiency of the promoter/BCD combination. Promoter/BCD combinations ranged from a low score of 6.14 to a high score of 634.54. The score for this combination was: 40.4
Proper citation: RRID:Addgene_47823 Copy
Species: Synthetic
Genetic Insert: Codon-optimised RH2b (partial)
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24043421
Proper citation: RRID:Addgene_47778 Copy
Genetic Insert: PesaR-luxCDABE-term
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:2934; Vector Backbone:pET-17b; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23879176
Comments: PesaR-luxCDABE-transcriptional terminator fragment from pCS-PesaRlux is between XhoI and BglII sites in pET17b.
PesaR (274bp, indicated as lowercase letters in the 2nd partial sequence for PesaR between XhoI and BamHI) is located between XhoI and BamHI sites. luxCDABE is located between two NotI sites. The transcriptional terminator is located downstream of luxCDABE between the second NotI and BglII.
Sequencing primers provided were used to sequence PesaR.
Proper citation: RRID:Addgene_47802 Copy
Species: Homo sapiens
Genetic Insert: PMCA4b
Vector Backbone Description: Backbone Marker: Adamo, H.P. et al. (1992) Biochem. J. 285, 791-797; Vector Backbone:pMM2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1323273
Comments: Note that PMCA4 residue 2 is Ala (instead of Thr) due to an optimized initiation sequence (ATG GCG).
Proper citation: RRID:Addgene_47759 Copy
Species: Synthetic
Genetic Insert: dCas9-VP64
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23892898
Comments: Plasmid Features:
Cas9 = 409-4512
NLS = 4519-4539
3X FLAG = 4546-4611
VP64 = 4633-4794
Proper citation: RRID:Addgene_47753 Copy
Species: Synthetic
Genetic Insert: codon optimized dCas9-VP64
Vector Backbone Description: Backbone Marker:Joung lab (Addgene plasmid # 43861); Vector Backbone:pJDS246; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23892898
Comments: Plasmid Features:
Cas9 = 409-4512
NLS = 4519-4539
3X FLAG = 4546-4611
VP64 = 4633-4794
Proper citation: RRID:Addgene_47754 Copy
Species: Synthetic
Genetic Insert: Codon-optimised EBA140
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24043421
Proper citation: RRID:Addgene_47742 Copy
Species: Synthetic
Genetic Insert: Codon-optimised AMA1
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24043421
Proper citation: RRID:Addgene_47741 Copy
Species: Synthetic
Genetic Insert: Codon-optimised MSP3
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24043421
Proper citation: RRID:Addgene_47731 Copy
Species: Synthetic
Genetic Insert: Codon-optimised PF3D7_1136200
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24043421
Proper citation: RRID:Addgene_47730 Copy
Species: Synthetic
Genetic Insert: Codon-optimised Pf113
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24043421
Proper citation: RRID:Addgene_47729 Copy
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